Enzyme histochemistry of uterine tube of buffalo during follicular and luteal phase: A seasonal variation

TULUMONI  SEAL; ANURADHA  GUPTA; VARINDER  UPPAL; DEVENDRA  PATHAK; NEELAM  BANSAL

doi:10.56093/ijans.v95i1.156835

Authors

TULUMONI SEAL College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004
ANURADHA GUPTA College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004
VARINDER UPPAL College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004
DEVENDRA PATHAK College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004
NEELAM BANSAL College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004

https://doi.org/10.56093/ijans.v95i1.156835

Keywords:

Buffalo, Histoenzyme, Summer uterine tube, Winter

Abstract

Enzyme histochemistry on uterine tube of buffalo during summer and winter season was conducted in different phases of estrus cycle in relation to reproductive efficiency. Fresh tissues of buffalo uterine tubes were collected from infundibulum, ampulla and isthmus immediately after slaughtering. Cryostat sections were obtained and incubated to find variation in localization of various enzymes. AKPase showed strong to intense activity in winter and strong during summer season in the lamina epithelialis whereas negligible to weak reaction was found in lamina propria, propria submucosa, tunica muscularis and tunica serosa of uterine tube during both the seasons. Succinic dehydrogenase showed strong reaction in winter and moderate to strong during summer in the lamina epithelialis of uterine tube whereas reaction was moderate to strong in tunica muscularis, weak in the tunica serosa and negligible to weak in the lamina propria of uterine tube during both the seasons. G-6-P-D showed strong to intense reaction during winter and summer in the lamina epithelialis of uterine tube whereas reaction was negligible to weak in lamina propria, weak in propria submucosa, moderate to strong in the tunica muscularis and negligible to weak in the tunica serosa of uterine tube. NADH-diaphorase and NADPH- diaphorases showed intense reaction during both summer and winter in lamina epithelialis, moderate to strong reaction in tunica muscularis, weak to moderate reaction in lamina propria and propria submucosa, and weak reaction in tunica serosa of infundibulum, ampulla and isthmus during both the seasons. The activity of the enzymes was found to be more in winter and in luteal phase as compared to summer and follicular phase.

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References

Abdalla O. 1968. Observations on the morphology and histochemistry of the oviducts of the sheep. Journal of Anatomy 102(2): 333-44.

Barka T and Anderson P J. 1963. Histochemistry: Theory, Practice and Bibliography. Pp. 273-316. Harper and Row Publishers, Inc., New York.

Basic Animal Husbandry Statistics. 2023. Animal Husbandry Statistics Division. Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Government of India.

Björkman N and Fredricsson B. 1961. The bovine oviduct epithelium and its secretory process as studied with the electron microscope and histochemical tests. Zeitschriftfür Zellforschung und Mikroskopische Anatomie 55(4): 500-13.

Chayen J. et al. 1969. A Guide for Practical Histochemistry. Oliver and Boyol, Edinburgh, England. pp 207.

Foraker A G and Wingo W J. 1956. Senile and seborrheic keratoses: Localization of succinic dehydrogenase, protein- bound sulfhydryl, and disulfide groups. The American Journal of Pathology 32(3): 521-33.

Hollis D E, Frith P A, Vaughan J D, Chapman R E and Nancarrow C D. 1984. Ultrastructural changes in the oviductal epithelium of Merino ewes during the estrous cycle. American Journal of Anatomy 171(4): 441-56.

Khillare G S, Sastry K V H, Singh R P, Agrawal R and Mohan J. 2013. Acid and alkaline phosphatase enzyme activity in sperm storage tubules in Japanese quail. Indian Journal of Poultry Science 48: 128-30.

Madan M L. 1988. Status of reproduction in female buffalo. Buffalo Production and Health: A compendium of latest research information based on Indian studies, pp. 89–100.

Madan M L. 1990. Factors limiting superovulation responses in embryo transfer programs among buffaloes. Theriogenology 33(1): 280.

Mishra O P et al. 2003. Study on biochemical constituents of caprine ovarian follicular fluid after superovulation. Asian- Australasian Journal of Animal Sciences 16(12): 1711-15.

Mokhtar D M. 2015. Microscopic and histochemical characterization of the bovine uterine tube during the follicular and luteal phases of estrous cycle. Journal of Microscopy and Ultrastructure 3(1): 44-52.

Motta P M and E SE Hafez. 1980. Biology of the Ovary, 1st ed. Martinus Nijhoff Publishers, London, England. p. 33-150.

Pearse A G E. 1972. Histochemistry: Theoretical and Applied, 3rd Edition, pp. 475-575. Churchill Livingstone.

Rama Rao A V S S. 1994. Textbook of Biochemistry. UBS Publishers, New Delhi. Pp 168.

Sorensen V W and Singh U B. 1973. On mitochondrial inclusion in granulosa lutein cells of pregnant cows. Experientia 29: 592-98.

Sukhadeve SV and Bansal. 2017. Localization of phosphatases, oxidoreductases and esterases in different segments of oviduct in laying Punjab white quail. Journal of Experimental Biology and Agricultural Sciences 5(4): 557-60.

Uppal V and Roy K S. 2007. Histoenzymatic studies on the oviduct during prenatal life in buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 77: 376-80.

Vale W G. 2007. Effects of environment on buffalo reproduction. Italian Journal of Animal Sciences 6(2):130–42.

Zamiri M J and Blackshaw A W. 1979. Enzyme histochemistry of the sheep uterus during the oestrous cycle. Australian Journal of Biological Sciences 32(5): 409-14.