PCR based identification of different strongyle species in goats of western Uttar Pradesh, India

RAMAKANT; TARUN KUMAR  SARKAR; AMIT KUMAR  VERMA; PREM SAGAR  MAURYA; VIKAS  JAISWAL; ARBIND  SINGH; VIPUL  THAKUR; SHRIYA  RAWAT

doi:10.56093/ijans.v95i1.157162

Authors

RAMAKANT College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, Uttar Pradesh
TARUN KUMAR SARKAR College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
AMIT KUMAR VERMA College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
PREM SAGAR MAURYA College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
VIKAS JAISWAL College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
ARBIND SINGH College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
VIPUL THAKUR College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110
SHRIYA RAWAT College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110

https://doi.org/10.56093/ijans.v95i1.157162

Keywords:

Faecal culture, Goat, Haemonchus, Oesophagostomum, PCR, Trichostrongylus

Abstract

Strongyle infection in goats poses a significant threat to the agricultural industry leading to substantial economic losses through decreased productivity and increased mortality rates. The current study’s goal is to determine the molecular- based methods for identification of different kind of strongyle eggs from naturally infected goats from four selected districts of Western Uttar Pradesh. A total of 360 faecal samples were collected, with 32.22% testing positive for strongyle infection. The positive faecal samples were pooled separately and subjected to larval culture, followed by DNA extraction from the L3 larvae. Polymerase chain reaction (PCR) analysis of the ITS-2 region of the rDNA confirmed the presence of Haemonchus contortus, Trichostrongylus and Oesophagostomum genera. The prevalence rates of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. were determined to be 54.16%, 31.66% and 14.16% respectively. The finding of this study highlighted Haemonchus contortus as the predominant species among the identified strongyle spp. These findings provide valuable insights into the distribution of different types of strongyle infection in goats, which can help in management of parasites and their control in goats.

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References

Airs P M, Cordero J V, Mvula W, Takahashi T, Wyk J V, Nalivata P, Safalaoh A and Morgan E R. 2023. Low-cost molecular methods to characterise gastrointestinal nematode co- infections of goats in Africa. Parasites and Vectors 16: 216. DOI: https://doi.org/10.1186/s13071-023-05816-y

Bott N J, Campbell E B, Beveridge I, Chilton N B, Ress D, Hunt P W and Gasser R B. 2009. A combined microscopic- molecular method for the diagnosis of Strongylide infections in sheep. International Journal for Parasitology 39: 1277-87. DOI: https://doi.org/10.1016/j.ijpara.2009.03.002

Callaghan M J and Beh K J. 1994. Characterization of a tandemly repetitive DNA-sequence from Haemonchus contortus. International Journal for Parasitology 24: 137-41. DOI: https://doi.org/10.1016/0020-7519(94)90068-X

Das M, Laha R, Goswami A and Sen A. 2017. Gastrointestinal parasitism of goats in hilly region of Meghalaya, India. Veterinary World 10(1): 81-85. DOI: https://doi.org/10.14202/vetworld.2017.81-85

Elseadawy R, Abbas I, Al-Araby M and Abu-Elwafa S. 2021. Molecular identification of different Trichostrongylus species infecting sheep and goats from Dakahlia governorate, Egypt. Journal of parasitic Diseases 45(1): 218-27. DOI: https://doi.org/10.1007/s12639-020-01299-1

Han T, Wang M, Zhang G, Han D, Li X, Liu G, Li X and Wang Z. 2017. Gastrointestinal nematodes infections and anthelmintic resistance in grazing sheep in the Eastern Inner Mongolia in China. Acta Parasitologica 62: 815-22. DOI: https://doi.org/10.1515/ap-2017-0098

Hassan S A, Mohammed A A and Omer F A. 2017. Molecular Method to Diagnosis of some Strongylide Nematode of goats in Nyala Area South Darfur State- Sudan. IOSR Journal of Agriculture and Veterinary Science 10(4): 54-56. DOI: https://doi.org/10.9790/2380-1004025456

Heise M, Epe C and Schnieder T. 1999. Differences in the Second Internal Transcribed spacer (ITS-2) of eight species of Gastrointestinal Nematodes of Ruminants. The Journal of Parasitology 85(3): 431-35. DOI: https://doi.org/10.2307/3285774

Kalule F, Vudriko P, Nanteza A, Ekiri A B, Alafiatayo R, Betts J, Betson M, Mijten E, Varga G and Cook A. 2023. Prevalence of gastrointestinal parasites and molecular identification of beta- tubulin mutations associated with benzimidazole resistance in Haemonchus contortus in goats from selected districts of Uganda. Veterinary Parasitology Regional Studies and Reports 42:100889. DOI: https://doi.org/10.1016/j.vprsr.2023.100889

Kochewad S A, Chavan S B, Kumar N, Tayade A S, Dhumal, N, Jadhavar P and Reddy K S. 2023. Self-sustaining goat farming model for livelihood improvement of small and marginal farmers. Technical Bulletin No.40. ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, Maharashtra India, pp. 1-25.

Kumar B D, Sankar M, Kumar R, Kumar A, Kadian P, Kushwaha B, Parthasarathi B C, Khare R K and Chandra D. 2018. Molecular Identification of Oesophagostomum spp. from Himalayan Grey Langur. International Journal of Current Microbiology and Applied Sciences 7(3): 146-51. DOI: https://doi.org/10.20546/ijcmas.2018.703.017

Muchiut S, Silvina F, Paula D, Eliana R, Edgardo R, Pedro S, Gisele B and Cesar F. 2021. Influence of faecal culture media and incubation time on the yield of infective larvae of Haemonchus contortus (Rudolphi 1803). Helminthology 120: 1493-97. DOI: https://doi.org/10.1007/s00436-021-07078-y

Nath T C, Lee D, Park H, Choe S, Ndosi B A, Kang Y, Bia M M, Eamudomkarn C, Mohanta U K, Islam K M, Bhuiiyan J U, Jeon H K and Eom K S. 2021. Morphometrical and molecular characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) isolated from goat (Capra hircus) in Sylhet, Bangladesh. Journal of Parasitology Research 3: 1-9. DOI: https://doi.org/10.1155/2021/8863283

Nisbet A J, Meeusen E N, González J F and Piedrafita D M. 2016. Immunity to Haemonchus contortus and vaccine development. Advances in Parasitology 93: 353-96. DOI: https://doi.org/10.1016/bs.apar.2016.02.011

Ntonifor H, Shei S, Ndaleh N and Mbunkur G. 2013. Epidemiological studies of gastrointestinal parasitic infections in ruminants in Jakiri, Bui division, North West region of Cameroon. Journal of Veterinary Medicine and Animal Health 5(12): 344-52.

Paul B T, Biu A A, Ahmed G M, Mohammed A, Philip M H and Yusuf J. 2016. Point prevalence and intensity of gastrointestinal parasite ova/ oocyst and its association with body condition score (BCS) of sheep and goats in Maiduguri, Nigeria. Journal of Advances in Parasitology 3: 81-88. DOI: https://doi.org/10.14737/journal.jap/2016/3.3.81.88

Rajpoot J, Shukla S, Jatav G P, Garg U K and Agrawal V. 2017. Coproculture study of strongyle infection of goats from Malwa region of Madhya Pradesh. Journal of Entomology and Zoology Studies 5(5): 876-78.

Roberts F H S and Sullivan P J O. 1950. Methods for egg counts and larval cultures for strongyles infesting the gastrointestinal tract of cattle. Australian Journal of Agricultural Research 1: 99-102. DOI: https://doi.org/10.1071/AR9500099

Roeber F, Jex A R and Gasser R B. 2013. Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance-an Australian perspective. Parasites and Vectors 6(1): 1-13. DOI: https://doi.org/10.1186/1756-3305-6-153

Roeber F, Jex A R, Campbell A J D, Campbell B E, Anderson G A and Gasser R B. 2011. Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections. Infection Genetics and Evolution 11: 849-54. DOI: https://doi.org/10.1016/j.meegid.2011.01.013

Sankar M, Vaishali, Silamparasan M, Prasanth V, Siddharth G, Ajayta, Singh N, Agri H, Rai V and Daria S. 2020. Prevalence of gastrointestinal strongyles of goats in mid himalayas of Uttarakhand, India. International Journal of Current Microbiology and Applied Sciences 9(3): 501-07. DOI: https://doi.org/10.20546/ijcmas.2020.903.058

Santos L L, Salgado J A, Drummond MG, Bastianetto E, Santos C P, Bruno S A F, Brasil, Taconeli C A, Denise A A and Oliveira 2020. Molecular method for the semi-quantitative identification of gastrointestinal nematodes in domestic ruminants. Parasitology Research 119: 529-43. DOI: https://doi.org/10.1007/s00436-019-06569-3

Sebro E, Kebamo M and Abebe A. 2022. Prevalence of gastrointestinal parasites of sheep and goats in An-Lemo, Hadiya Zone Southern Ethiopia. Indian Journal of Science and Technology 15(22): 1084-90. DOI: https://doi.org/10.17485/IJST/v15i22.1384

Singh A K, Das G, Roy B, Nath S, Naresh R and Kumar S. 2014. Prevalence of Strongyle infections in goat of Maha Koushal region, Madhya Pradesh, India. Journal of Parasitic Diseases 40(2): 289–91. DOI: https://doi.org/10.1007/s12639-014-0496-5

Soulsby E J L. 1982. Helminths, Arthropods and Protozoa of Domesticated Animals, 7th edn. Baillière Tindall: London.

Tripathi B N, Kumar A, Dixit A K, Rai B and Saxena V K. 2020. Improvement in goat production for sustainable rural livelihood. Mahatma Gandhi’s Vision of Agriculture: Achievements of ICAR. Directorate of Knowledge Management in Agriculture, Indian Council of Agricultural Research, Krishi Anusandhan Bhawan I, Pusa, New Delhi. pp. 39-47.

Tsotetsi A and Mbati P. 2003. Parasitic helminths of veterinary importance in cattle, sheep and goats on communal farms in the North eastern Free State, South Africa. Journal of the South African Veterinary Association 74(2): 45-48. DOI: https://doi.org/10.4102/jsava.v74i2.503

Veena M, Puttalakshmamm G C K, Ananda J and Sengupta P P. 2020. Morphological and molecular characterization of gastrointestinal nematodes in sheep. International Journal of Current Microbiology and Applied Sciences 9(4): 2564-75. DOI: https://doi.org/10.20546/ijcmas.2020.904.308

Waghorn T S, Knight J S and Leathwick D M. 2013. The distribution and anthelmintic resistance status of Trichostrongylus colubriformis, T. vitrinus and T. axei in lambs in New Zealand. New Zealand Veterinary Journal 62(3): 152-59. DOI: https://doi.org/10.1080/00480169.2013.871193

Waller P and Chandrawathani P. 2005. Haemonchus contortus: parasite problem no.1 from tropics-Polar Circle. Problems and prospects for control based on epidemiology. Tropical Biomedicine 22 (2): 131-37.

Zvinorova P I, Halimani T E, Muchadeyi F C, Matika O, Riggio V and Dzama K. 2016. Prevalence and risk factors of gastrointestinal parasitic infections in goats in low-input low-output farming systems in Zimbabwe. Small Ruminant Research 143: 75-83. DOI: https://doi.org/10.1016/j.smallrumres.2016.09.005