Study on prevalence of ESBL producing multi drug resistant E. coli in livestock and poultry in Patna district of Bihar state, India

ARCHANA; PURUSHOTTAM  KAUSHIK; ANJAY; BHOOMIKA; SEULI SAHA  ROY; BABLU  KUMAR; ANIL  KUMAR; MANOJ  KUMAR; SAVITA  KUMARI; SAURABH  SWAMI

doi:10.56093/ijans.v95i4.157437

Authors

ARCHANA Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
PURUSHOTTAM KAUSHIK Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
ANJAY Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
BHOOMIKA Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
SEULI SAHA ROY Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
BABLU KUMAR Indian Veterinary Research Institute, Izatnagar, India
ANIL KUMAR Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
MANOJ KUMAR Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
SAVITA KUMARI Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India
SAURABH SWAMI Bihar Veterinary College, Bihar Animal Sciences University, Patna 800014, India

https://doi.org/10.56093/ijans.v95i4.157437

Keywords:

AMR, Animal, E. coli, ESBL, MDR

Abstract

Antimicrobial resistance is considered one of the greatest public health threat undermining the effectiveness of antibiotics. Occurrence of Extended spectrum beta-lactamase (ESBL) producing multidrug resistant (MDR) E. coli in human and animal poses a clinical and epidemiological challenge. The aim of the present study was to determine the occurrence of ESBL-producing MDR E. coli among healthy livestock and chicken and to investigate the types of ESBL genes circulating among livestock in Patna district of Bihar. A total of 254 samples from apparently healthy animals and chickens were collected from sixteen villages of Patna district which comprised of raw milk, rectal swabs and chicken cloacal swab. Out of 254 samples processed a total 148 isolates were confirmed as E. coli. After isolation and confirmation of E. Coli resistance profile were generated by disc diffusion method. The antimicrobial resistance profile of isolates revealed that 85.13% isolates were resistant to amoxicillin/ clavulanate; 72.97% to cefpodoxime; 37.83% resistant to ampicillin; 33.10% to ceftazidime; 27.02% to nalidixic acid; 26.35% to cefoxitin; and 23.64% to trimethoprim. However, 97.98% isolates were found susceptible to amikacin and 100% were sensitive to imipenem. The MDR analysis showed that 54.05% of isolates have MAR index ≥0.25 and 8.1% of isolates have MAR index ≥0.5. Further, out of 148 E. coli isolates tested for ESBL production, 30 isolates were found ESBL producer. The study provides data on prevalence of MDR ESBL producing E. coli among livestock in Bihar, India, indicating the risk of transmission of pathogens to humans through horizontal transmission.

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References

Agrawal S, Singh A P, Singh R, Saikia R, Choudhury S, Shukla A, Prabhu S N and Agrawal J. 2021. Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India. Veterinary World 14: 200-09.

Athanasakopoulou Z, Reinicke M, Diezel C, Sofia M, Chatzopoulos D C, Braun S D, Reissig A, Spyrou V, Monecke S, Ehricht R and Tsilipounidaki K. 2021. Antimicrobial resistance genes in ESBL- producing Escherichia coli isolates from animals in Greece. Antibiotics 10(4): 389.

Bailar III J C and Travers K. 2002. Review of assessments of the human health risk associated with the use of antimicrobial agents in agriculture. Clinical Infectious Diseases 34(Suppl3): S135-S143.

Banerjee J, Bhattacharyya D, Habib M, Chaudhary S, Biswas S, Maji C, Nanda P K, Das A K, Dandapat P, Samanta I and Lorenzo J M. 2022. Antimicrobial resistance pattern, clustering mechanisms and correlation matrix of drug- resistant Escherichia coli in black Bengal Goats in West Bengal, India. Antibiotics 11(10): 1344.

Bennett P M. 2008. Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria. British Journal of Pharmacology 153: S347-S357.

Bhoomika, Shakya S, Patyal A and Gade N E. 2016. Occurrence and characteristics of extended-spectrum β-lactamases producing Escherichia coli in foods of animal origin and human clinical samples in Chhattisgarh, India. Veterinary World 9(9): 996-1000.

Blair J M, Webber M A, Baylay A J, Ogbolu D O and Piddock L J. 2015. Molecular mechanisms of antibiotic resistance. Nature Reviews Microbiology 13(1): 42-51.

Brower C H, Mandal S, Hayer S, Sran M, Zehra A, Patel S J, Kaur R, Chatterjee L, Mishra S, Das B R and Singh P. 2017. The prevalence of Extended-Spectrum Beta-Lactamase- producing Multidrug-Resistant Escherichia coli in poultry chickens and variation according to farming practices in Punjab, India. Environmental Health Perspectives 125(7): 077015.

Bush K and Bradford P A. 2020. Epidemiology of β-Lactamase- Producing Pathogens. Clinical Microbiology Reviews 33(2): e00047-19.

Carattoli A. 2008. Animal reservoirs for extended spectrum beta- lactamase producers. Clinical Microbiology and Infection Suppl 1: 117-23. doi: 10.1111/j.1469-0691.2007.01851.x.

CDDEP. 2020. The Center for Disease Dynamics, Economics & Policy. Resistance Map: Antibiotic resistance.

Dallenne C, Da Costa A, Decré D, Favier C and Arlet G. 2010. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. Journal of Antimicrobial Chemotherapy 65(3): 490-95.

Das A, Guha C, Biswas U, Jana P S, Chatterjee A and Samanta I. 2017. Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal. Veterinary World 10(5): 517-20.

Ewers C, Bethe A, Semmler T, Guenther S and Wieler L H. 2012. Extended-spectrum β-lactamase-producing and AmpC- producing Escherichia coli from livestock and companion animals, and their putative impact on public health: a global perspective. Clinical Microbiology and Infection 18(7): 646- 55.

Harwalkar A, Sataraddi J, Gupta S, Yoganand R, Rao A and Srinivasa H. 2013. The detection of ESBL-producing Escherichia coli in patients with symptomatic urinary tract infections using different diffusion methods in a rural setting. Journal of Infection and Public Health 6(2): 108-14.

Haulisah N A, Hassan L, Bejo S K, Jajere S M and Ahmad N I. 2021. High levels of antibiotic resistance in isolates from diseased livestock. Frontiers In Veterinary Science 8: 652351. Jacquier H, Birgy A, Le Nagard H, Mechulam Y, Schmitt E, Glodt J, Bercot B, Petit E, Poulain J, Barnaud G and Gros

P A. 2013. Capturing the mutational landscape of the beta- lactamase TEM-1. The Proceedings of the National Academy of Sciences USA 110(32): 13067-72.

Jain P, Bepari A K, Sen P K, Rafe T, Imtiaz R, Hossain M and Reza H M. 2021. High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate. Scientific Reports 11(1): 22859.

Jaiswal A, Khan A, Yogi A, Singh S, Pal A K, Soni R, Tripathi P, Lal J A and Tripathi V. 2024. Isolation and molecular characterization of multidrugresistant Escherichia coli from chicken meat. 3 Biotech 14(4): 107.

Jana A and Mondal A. 2013. Serotyping, pathogenicity and antibiogram of Escherichia coli isolated from raw poultry meat in West Bengal, India. Veterinaria Italiana 49(4): 361- 65.

Jindal P, Bedi J, Singh R, Aulakh R and Gill J. 2021. Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli and Klebsiella isolated from dairy farm milk, farm slurry and water in Punjab, India. Environmental Science and Pollution Research 28(22): 28556-70.

Kar D, Bandyopadhyay S, Bhattacharyya D, Samanta I, Mahanti A, Nanda P K, Mondal B, Dandapat P, Das A K, Dutta T K and Bandyopadhyay S. 2015. Molecular and phylogenetic characterization of multidrug resistant extended spectrum beta-lactamase producing Escherichia coli isolated from poultry and cattle in Odisha, India. Infection, Genetics and Evolution 29: 82-90.

Kaushik P, Anjay A, Kumari S, Dayal S and Kumar S. 2018. Antimicrobial resistance and molecular characterisation of E. coli from poultry in Eastern India. Veterinaria Italiana 54(3): 197-204.

Lalhruaipuii K, Dutta T K, Roychoudhury P, Chakraborty S, Subudhi P K, Samanta I, Bandyopadhayay S and Singh S B. 2021. Multidrug-resistant extended-spectrum β-lactamase- producing Escherichia coli pathotypes in north eastern region of India: Backyard small ruminants-human-water interface. Microbial Drug Resistance 27(12): 1664-71.

Larson E. 2007. Community factors in the development of antibiotic resistance. Annual Review of Public Health 28: 435- 47.

Martínez-Vázquez A V, Mandujano A, Cruz-Gonzalez E, Guerrero A, Vazquez J, Cruz-Pulido W L, Rivera G and Bocanegra-García V. 2022. Evaluation of retail meat as a source of ESBL Escherichia coli in Tamaulipas, Mexico. Antibiotics 11(12): 1795.

Mukherjee M, Basu S, Mukherjee S K and Majumder M. 2013. Multidrug-resistance and Extended Spectrum Beta-Lactamase production in Uropathogenic E. coli which were isolated from hospitalized patients in Kolkata. Journal of Clinical and Diagnostic Research 7(3): 449-53.

Murugan M S, Sinha D K, Kumar O V, Yadav A K, Pruthvishree B S, Vadhana P, Nirupama K R, Bhardwaj M and Singh B R. 2019. Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India. Epidemiology and Infection 147: e159.

Nobrega D B, Tang K L, Caffrey N P, De Buck J, Cork S C, Ronksley P E and Barkema H W. 2021. Prevalence of antimicrobial resistance genes and its association with restricted antimicrobial use in food producing animals: a systematic review and meta-analysis. Journal of Antimicrobial Chemotherapy 76(3): 561-75.

O’Neill J. 2016. Tackling drug-resistant infections globally: final report and recommendations. 1-84. 160518_Final paper_with cover.pdf (amr-review.org)

Odenthal S, Akineden Ö and Usleber E. 2016. Extended-spectrum β-lactamase producing Enterobacteriaceae in bulk tank milk from German dairy farms. International Journal of Food Microbiology 238: 72-78.

Prinarakis E E, Miriagou V, Tzelepi E, Gazouli M and Tzouvelekis L S. 1997. Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant. Antimicrobial Agents and Chemotherapy 41(4): 838-40.

Quinn P J, Carter M, Markey B and Carter G R. 1994. Clinical Veterinary Microbiology. Mosby Wolfe, Wolfe, UK.

Rahman M M, Husna A, Elshabrawy H A, Alam J, Runa N Y, Badruzzaman A T, Banu N A, Al Mamun M, Paul B, Das S and Rahman M M. 2020. Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat. Scientific Reports 10(1): 21999.

Ramadan A A, Abdelaziz N A, Amin M A and Aziz R K. 2019. Novel blaCTX-M variants and genotype-phenotype correlations among clinical isolates of extended spectrum beta lactamase-producing Escherichia coli. Scientific Reports 9(1): 4224.

Robin F, Krebs M, Delmas J, Gibold L, Mirande C and Bonnet R. 2011. In vitro efficiency of the piperacillin/tazobactam combination against inhibitor-resistant TEM- and complex mutant TEM-producing clinical strains of Escherichia coli. Journal of Antimicrobial Chemotherapy 66(5): 1052-56.

Rohit A, Deekshit V K, Balaraj M, Alandur V S, Abraham G, Karunasagar I and Karunasagar I. 2019. CTX-M type extended-spectrum β-lactamase in Escherichia coli isolated from extra-intestinal infections in a tertiary care hospital in South India. Indian Journal of Medical Research 149(2): 281-84.

Sabat G, Rose P, Hickey W J and Harkin J M. 2000. Selective and sensitive method for PCR amplification of Escherichia coli 16S rRNA genes in soil. Applied and Environmental Microbiology 66(2): 844-49.

Samanta I, Joardar S N, Das P K, Das P, Sar T K, Dutta T K, Bandyopadhyay S, Batabyal S and Isore D P. 2014. Virulence repertoire, characterization, and antibiotic resistance pattern analysis of Escherichia coli isolated from backyard layers and their environment in India. Avian Diseases 58(1): 39-45.

Suay-García B and Pérez-Gracia M T. 2019. Present and future of carbapenem-resistant Enterobacteriaceae infections. Antibiotics 8(122): 1-16.

Suay-García B, Galán F, Rodríguez-Iglesias M A and Pérez-Gracia M T. 2019. Detection and characterization of Extended-Spectrum Beta-Lactamases-producing Escherichia coli in animals. Vector-Borne and Zoonotic Diseases 19(2): 115-20.

Tan T Y, Ng L S, He J, Koh T H and Hsu L Y. 2009. Evaluation of screening methods to detect plasmid-mediated AmpC in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Antimicrobial Agents and Chemotherapy 53(1): 146-49.

Tewari R, Ganaie F, Venugopal N, Mitra S, Shome R and Shome B R. 2022. Occurrence and characterization of genetic determinants of β-lactam-resistance in Escherichia coli clinical isolates. Infection, Genetics and Evolution 100: 105257.

Tewari R, Mitra S, Ganaie F, Das S, Chakraborty A, Venugopal N, Shome R, Rahman H and Shome B R. 2019. Dissemination and characterisation of Escherichia coli producing extended- spectrum β-lactamases, AmpC β-lactamases and metallo- β-lactamases from livestock and poultry in Northeast India: A molecular surveillance approach. Journal of Global Antimicrobial Resistance 17: 209-15.

Ur Rahman S, Ali T, Ali I, Khan N A, Han B and Gao J. 2018. The growing genetic and functional diversity of Extended Spectrum Beta-Lactamases. BioMed Research International 9519718.

Van Boeckel T P, Pires J, Silvester R, Zhao C, Song J, Criscuolo N G, Gilbert M, Bonhoeffer S and Laxminarayan R. 2019. Global trends in antimicrobial resistance in animals in low- and middle-income countries. Science 365(6459): eaaw1944.

WHO. 2021. WHO integrated global surveillance on ESBL- producing E. coli using a “One Health” approach: Implementation and opportunities. ISBN 978-92-4-002140-2 (electronic version).