Study of bacterial haemorrhagic septicaemia causing mortality in Indian major carps from culture ponds of Kolleru lake, Andhra Pradesh, India

GOWRI DEVI  K; SHARMILA  KHAN; VIJAY KUMAR P  P N; UMMEY  SHAMEEM; RUDDARAJU LAKSHMI  KALYANI

doi:10.56093/ijans.v95i5.158143

Authors

GOWRI DEVI K Swami Shraddhanand College, University of Delhi, Alipur, Delhi – 110036 India
SHARMILA KHAN Andhra University, Visakhapatnam, Andhra Pradesh 530 003 India
VIJAY KUMAR P P N Andhra University, Visakhapatnam, Andhra Pradesh 530 003 India
UMMEY SHAMEEM Andhra University, Visakhapatnam, Andhra Pradesh 530 003 India
RUDDARAJU LAKSHMI KALYANI Omega College of Pharmacy, Edulabad, Ghatkesar, Telangana 501301

https://doi.org/10.56093/ijans.v95i5.158143

Keywords:

Aeromonas hydrophila, C. catla, Haemorrhagic septicaemia, Kolleru Lake, L. rohita

Abstract

Haemorrhagic septicaemia (HS) is a bacterial disease causing severe economic losses in freshwater aquaculture. The present study aimed to isolate, identify, and characterize its pathogen responsible for disease outbreaks and mortality in Indian major carps from ponds in the Kolleru Lake region, India through monthly sampling over eight months. Infected fish exhibited hemorrhages on the body, around the eyes, mouth, ventral surface, fins, and caudal region. Blood, kidney, liver, spleen, and gill tissues were processed for microbiological, biochemical, and molecular analysis. DNA from the isolates was amplified via PCR using primers specific to Aeromonas hydrophila, yielding a ~760 bp product, confirming the pathogen’s presence. This finding establishes A. hydrophila as the causative agent of haemorrhagic septicaemia in Labeo rohita and Catla catla. The results underscore the frequent and significant mortalities in culture ponds caused by bacterial diseases such as haemorrhagic septicaemia and abdominal dropsy, highlighting the need for targeted disease management strategies in aquaculture.

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