Effect of superstimulation on follicular growth in Hariana and Sahiwal cows under different OPU schedules

A  KUMAR; A  SAXENA; M  ANAND; A  MISHRA; P  SRIVASTAVA; A  PRIYADERSHI

doi:10.56093/ijans.v95i9.163477

Authors

A KUMAR U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India
A SAXENA U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India
M ANAND U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India
A MISHRA U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India
P SRIVASTAVA U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India
A PRIYADERSHI U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India

https://doi.org/10.56093/ijans.v95i9.163477

Keywords:

eCG, OPU, Follicular dynamics, Hariana, Indigenous breeds, Sahiwal, Superstimulation

Abstract

Superstimulation using exogenous equine chorionic gonadotropin (eCG) can enhance ovarian follicular development, which is crucial for improving reproductive outcomes in cattle. This investigation evaluated the impact of eCG (1000 IU, intramuscular) on follicular dynamics in Hariana and Sahiwal cows under weekly and bi-weekly ovum pick-up (OPU) protocols. Twelve repeat-breeding cows (n=6 Hariana, n=6 Sahiwal) were assigned to either weekly or bi-weekly OPU protocols (3 Hariana and 3 Sahiwal per schedule). Ovarian follicles were classified as small (<4 mm), medium (4–8 mm), and large (>8 mm) and counted by ultrasonography before and 72 hours after eCG injection. Statistical comparisons were performed using one-way ANOVA, with significance set at P<0.05 and P<0.01. Results revealed a significant increase in medium and large follicles post-superstimulation in both breeds, with Hariana cows showing a more robust increase in large follicles. These findings support the need for tailored superstimulation protocols for indigenous breeds to maximize oocyte yield.

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