A cross-sectional study on swine brucellosis sero-survey in Nagaland state of North Eastern India

RAJESWARI  SHOME; TRIVENI  KALLESHAMURTHY; SOMY  SKARIAH; G. SHANMUGAM; NAGALINGAM  MOHANDOSS; BIBEK RANJAN  SHOME; MOHAMMED MUDASSAR  CHANDA; DIVAKAR  HEMADRI; SHARANAGOUDA S  PATIL

doi:10.56093/ijans.v95i10.164420

Authors

RAJESWARI SHOME ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
TRIVENI KALLESHAMURTHY ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
SOMY SKARIAH ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
G. SHANMUGAM ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
NAGALINGAM MOHANDOSS ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
BIBEK RANJAN SHOME ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
MOHAMMED MUDASSAR CHANDA ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
DIVAKAR HEMADRI ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064
SHARANAGOUDA S PATIL ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka-560 064

https://doi.org/10.56093/ijans.v95i10.164420

Keywords:

iELISA, India, Nagaland, Swine brucellosis, Sero-epidemiology

Abstract

Swine brucellosis caused by the bacterium Brucella suis, is an important zoonotic disease characterized by abortion, birth of dead or weak piglets, orchitis in males, lameness and sometimes paralysis. Brucellosis is endemic to many Indian states but disease status is unknown in the pig-rearing state of Nagaland in North-Eastern India. The present study aimed to survey presence of anti-Brucella antibodies in pigs to understand the serological status of disease. A cross-sectional study design was adopted resulting in collection of 1550 serum samples from nine out of eleven districts, 43 out of 74 blocks (58.10% of blocks) and 59 out of 1428 epiunits called villages (4.13% of epiunits) and serum samples were tested by in-house developed indirect ELISA. In the study, more males (n-932) were tested compared to female pigs (n-618) which is approximately in the ratio of 3:2. Overall, 6.84% (5.69-8.2) apparent prevalence for brucellosis (AP) was noted with the highest sero-prevalence of 53.77% in Wokha district followed by Zunhebot, Tuensang and Kiphire districts as 6.82, 5.22 and 4.14%, respectively and least sero-positivity (2%) in Dimapur (the largest district) and Kohima district (the capital of Nagaland). At epiunit level, 100% sero-prevalence was recorded in two epiunits, >15% in three epiunits and >10% prevalence in four epiunits. Non-significantly higher sero-prevalence was recorded in males (7.40%) alongwith significantly higher prevalence in 24-month-old pigs (17.85%). The study identified swine brucellosis endemic regions in the state of Nagaland, which will be helpful for planners in implementing intervention measures in the absence of a vaccination policy for pigs in the country.

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References

Boral R, Singh M and Singh D K. 2009. Status and strategies for control of brucellosis-A review. Indian Journal of Animal Sciences 79(12): 1191–99.

Department of Animal Husbandry, Dairying and Fisheries (DAHD). 2019. All India report of 20th Livestock Census. Ministry of Agriculture, Government of India. https://dahd. nic.in/sites/default/filess/Key%20Results%2BAnnexure%20 18.10.2019.pdf.

Deqiu S, Donglou X and Jiming Y. 2002. Epidemiology and control of brucellosis in China. Veterinary Microbiology 90(1-4): 165–82. https://doi.org/10.1016/s0378-1135(02)00252-3.

Department of Animal Husbandry and Veterinary Services (AH&VS). 2016. Nagaland pig breeding policy. Government of Nagaland. https://cgspace.cgiar.org/bitstream/handle/10568/76530/nagaland_pig_breeding_policy_2016.pdf?sequence=1&isAllowed=y. Accessed 16 March 2022.

Jonsson E. 2013. Sero-prevalence and risk factors for bovine brucellosis, salmonellosis and bovine viral diarrhea in urban and peri-urban areas of Kampala, Uganda. Swedish University of Agricultural Sciences, Uppsala, Sweden. http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-2002. Accessed 31 January 2023.

Jindal P, Singh B B, Kaur P and Gill J P S. 2017. Sero-prevalence and molecular detection of Brucella species in slaughter pigs (Sus scrofa) of Punjab, India. Journal of Animal Research 7(3): 495–99. http://doi.org/10.5958/2277-940X.2017.00073.0.

Kaur A, Mahajan V, Singh N D, Filia G, Banga H S, Leishangthem G D and Singh A G. 2020. Patho-epidemiological and molecular diagnosis of swine brucellosis in Punjab. Indian Journal of Animal Research 54(1): 90–95. http://doi. org/10.18805/ijar.B-3728.

Mai H M, Irons P C, Kabir J and Thompson P N. 2012. A large sero-prevalence survey of brucellosis in cattle herds under diverse production systems in Northern Nigeria. BMC Veterinary Research 8: 144. https://doi.org/10.1186/1746-6148-8-144.

McDermott J, Grace D and Zinsstag J. 2013. Economics of brucellosis impact and control in low-income countries. Revue Scientifique et Technique 32(1): 249–61. https://doi. org/10.20506/rst.32.1.2197.

Nath A J, Barman N N, Sarma D K, Das S K and Bora D P. 2009. Sero-prevalence of brucellosis and parvovirus infections in pigs. Indian Veterinary Journal 86 (4): 339–41.

Office International des Epizooties (OIE). 2018. Terrestrial Manual. Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis and B. suis). https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf. Accessed 20 October 2022.

Olsen S C, Garin-Bastuji B, Blasco J M, Nicola A M and Samartino L. 2012. Diseases of Swine. (Eds) Zimmerman J J, Karriker L A, Ramirez A, Schwartz K J and Stevenson G W. 10th edition, Wiley-Blackwell, Hokoben, pp. 697–708.

Olsen S C and Palmer M V. 2014. Advancement of knowledge of Brucella over the past 50 years. Veterinary Pathology 51(6): 1076–89. https://doi.org/10.1177/0300985814540545.

Olsen S C and Tatum F M. 2017. Swine brucellosis: current perspectives. Veterinary Medicine: Research and Reports 8: 1–12. https://doi.org/10.2147/VMRR.S91360.

Olufemi O T, Dantala D B, Shinggu P A, Dike U A, Otolorin G R, Nwuku J A, Baba-Onoja E B T, Jatau T D and Amama F I. 2018. Sero-prevalence of brucellosis and associated risk factors among indigenous breeds of goats in Wukari, Taraba State, Nigeria. Journal of Pathology 5257926. https://doi. org/10.1155/2018/5257926.

Patra M K, Begum S and Deka B C. 2016. Problems and prospects of traditional pig farming for tribal livelihood in Nagaland. Indian Journal of Extension Education 14(4): 6–11.

Preena P, Ronald B S M, Balakrishnan S, Murugan M, Anbu K K and Ganesan P I. 2024. Serological, bacteriological, and molecular detection of brucellosis in pigs of Tamil Nadu, India, Emerging Animal Species 10: 100041. https://doi.org/10.1016/j.eas.2024.100041.

Rajkhowa S, Rahman H, Rajkhowa C and Bujarbaruah K M. 2005. Sero-prevalence of brucellosis in mithuns (Bos frontalis) in India. Preventive Veterinary Medicine 69(1-2): 145–51. https://doi.org/10.1016/j.prevetmed.2005.02.005.

Rogan W J and Rogan B. 1978. Estimating prevalence from the results of a screening test. American Journal of Epidemiology 107(1): 71–76. https://doi.org/10.1093/oxfordjournals.aje. a112510.

Shome R, Kalleshamurthy T, Natesan K, Jayaprakash K R, Byrareddy K, Mohandoss N, Sahay S, Shome B R, Hiremath J, Rahman H and Barbuddhe S B. 2019. Serological and molecular analysis for brucellosis in selected swine herds from Southern India. Journal of Infection and Public Health 12(2): 247–51. https://doi.org/10.1016/j.jiph.2018.10.013.

Shome R, Kalleshamurthy T, Nagaraj C, Rathore Y, Ramanjinappa K D, Skariah S, Mohandoss N, Shome B R , Chanda M M and Hemadri D . 2022. Countrywide cross-sectional study of swine brucellosis sero-prevalence in Indian subcontinent during 2018–2019. Tropical Animal Health Production 54(2):114. https://doi.org/10.1007/s11250-022-03107-9.

Shome R, Triveni K, Padmashree B S, Sahay S, Rao N, Shome B R, Misri J and Rahman H. 2016. Record of porcine brucellosis in India by indigenously developed indirect ELISA. Asian Pacific Journal of Tropical Diseases 6(11): 892–94. https:// doi.org/10.1016/S2222-1808(16)61153-2.

Thirlwall R E, Commander N J, Brew S D, Cutler S J, McGiven J A and Stack J A. 2008. Improving the specificity of immunodiagnosis for porcine brucellosis. Veterinary Research Communications 32: 209–13. https://doi.org/10.1007/s11259-007-9023-9.

Thrushfield M. 2005. Veterinary Epidemiology. Third edition, Blackwell Publishing Professional, Ames, Iowa, pp. 33.