Evaluation of aqueous coriander (Coriandrum sativum) seed extract asa natural anthelmintic against Haemonchus contortus in naturally infected goats

RAMAKANT; TARUN KUMAR  SARKAR; AMIT KUMAR  VERMA; PREM SAGAR  MAURYA; ARBIND  SINGH; VIPUL  THAKUR; PANKAJ KUMAR  MAURYA; SACHIN  GAUTAM; SHRIYA  RAWAT

doi:10.56093/ijans.v96i4.166216

Authors

RAMAKANT College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology Kumarganj 224 229 Ayodhya, Uttar Pradesh, India
TARUN KUMAR SARKAR College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, Uttar Pradesh, India
AMIT KUMAR VERMA College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, Uttar Pradesh, India
PREM SAGAR MAURYA College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, Uttar Pradesh, India
ARBIND SINGH College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, Uttar Pradesh, India
VIPUL THAKUR College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, Uttar Pradesh, India
PANKAJ KUMAR MAURYA College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology Kumarganj-224 229 Ayodhya, Uttar Pradesh, India
SACHIN GAUTAM College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology Kumarganj-224 229 Ayodhya, Uttar Pradesh, India
SHRIYA RAWAT College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India

https://doi.org/10.56093/ijans.v96i4.166216

Keywords:

Antioxidant status, Coriandrum sativum, Faecal egg count reduction test, Goat, Haemonchus contortus

Abstract

Gastrointestinal nematode infections, particularly those caused by Haemonchus contortus, remain a major constraint to goat production worldwide. The present study evaluated the dose-dependent anthelmintic efficacy of an aqueous seed extract of Coriandrum sativum in naturally infected goats and its effect on haematological, biochemical, and antioxidant parameters of the host. Thirty naturally infected goats (18–30 months old) with faecal egg counts (EPG) exceeding 1000 were randomly allocated to five groups (n = 6). Group C served as the untreated negative control, Group PC received closantel (10 mg/kg body weight, orally) as the positive control, and Groups CS-50, CS-250, and CS-500 received aqueous C. sativum seed extract at doses of 50, 250, and 500 mg/kg body weight, respectively. Blood and faecal samples were collected on days 0, 7, 14, 21, and 28 post-treatments. Highest faecal egg count reduction percentage was observed in the CS-500 group (40.38%) on day 28, whereas closantel achieved a complete faecal egg count reduction (100%). CS-500 also showed improvements in haemoglobin concentration, packed cell volume, total erythrocyte count, serum total protein, and albumin levels compared with those in the untreated goats. Antioxidant markers, showed statistically significant increasing trend in the treated animals. The findings demonstrated a dose-dependent anthelmintic effect of aqueous C. sativum seed extract against naturally acquired H. contortus infection, with the 500 mg/kg dose producing the greatest response among the tested doses. Although its efficacy was lower than that of closantel, the extract showed no adverse effects and may serve as a promising complementary phytotherapeutic option for the integrated management of gastrointestinal nematodes in goats.

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References

Adak M and Kumar P. 2022. Herbal anthelmintic agents: A narrative review. Journal of Traditional Chinese Medicine 42(4): 641-651.

Aebi H. 1984. Catalase In vitro. In: Methods in Enzymology (L. Packer, edn.). Academic press, New York., 105: 121–126. DOI: https://doi.org/10.1016/S0076-6879(84)05016-3

Ahmed A, Dar M A, Bhat A A, Jena B, Mishra G K and Tiwari R P. 2015. Study on haemato-biochemical profile in goats suffering from gastrointestinal parasitism in Jaipur district of Rajasthan. Journal of Livestock Science 6: 52–55.

Ahmed W M S, Mostafa A M, Al- Salahy M B, Wahba A A and Shehata O. 2019. The potential effect of Allium Sativum and Coriandrum Sativum as natural sources of antioxidants on Haemonchus contortus infection in sheep. Egyptian Veterinary Medical Society of Parasitology Journal 15: 89–113. DOI: https://doi.org/10.21608/evmspj.2019.81871

Ashfaque M, Khan S A, Fakhruddin Chahar A and Tanwar R K. 2016. Haemato-biochemical changes in goats suffering from helminth parasites infestation. Veterinary Practitioner 17(1): 55–56.

Athanasiadou S, Githiori J and Kyriazakis I. 2007. Medicinal plants for helminth parasite control: facts and fiction. Animal 2(9): 1392–1400. DOI: https://doi.org/10.1017/S1751731107000730

Balic A, Bowles V M and Meeusen E N. 2000. The immunology of gastrointestinal nematodes in ruminants. Advances in Parasitology 45: 181–241. DOI: https://doi.org/10.1016/S0065-308X(00)45005-0

Bandhaiya D, Aich R, Sharda R and Agrawal V. 2019. Biochemical parameters assessment in goats naturally infected with Haemonchus contortus. Journal of Entomology and Zoology Studies 7: 1316–1320.

Benzie E F I and Strain J J. 1999. Ferric reducing/antioxidant power assay: Direct measurement of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant and ascorbic acid concentration. Methods in Enzymology 299: 15–27. DOI: https://doi.org/10.1016/S0076-6879(99)99005-5

Coles G C, Bauer C, Berystede F H M, Greets S, Klei T R, Taylor M A and Waller P J. 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 44 (1-2): 35–44. DOI: https://doi.org/10.1016/0304-4017(92)90141-U

Dhama K, Chakraborty S, Mahima, Wani M Y, Verma A K, Deb R, Tiwari R and Kapoor S. 2013. Novel and emerging therapies safeguarding health of humans and their companion animals: A review. Pakistan Journal of Biological Sciences 16(3): 101–111. DOI: https://doi.org/10.3923/pjbs.2013.101.111

Eguale T, Tilahun G, Debella A, Feleke A and Makonnen E. 2007. In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus. Journal of Ethnopharmacology 110: 428–433. DOI: https://doi.org/10.1016/j.jep.2006.10.003

Good B, Hanrahan J P, de Waal D T, Patten T, Kinsella A and Lynch C O. 2012. Anthelmintic-resistant nematodes in Irish commercial sheep flocks- the state of play. Iranian Veterinary Journal 65(1): 21. DOI: https://doi.org/10.1186/2046-0481-65-21

Kaur A, Kaur N, Chauhan K, Jyoti, Singh H and Singh NK. 2025. Epidemiology of caprine gastrointestinal nematodes and associated efficacy of anthelmintic drugs in Punjab districts, India. Scientific Reports 15: 5211. DOI: https://doi.org/10.1038/s41598-025-89784-6

Laribi B, Kouki K, Hamdi M and Bettaieb T. 2015. Coriander (Coriandrum sativum L.) and its bioactive constituents. Fitoterapia 103: 9–26. DOI: https://doi.org/10.1016/j.fitote.2015.03.012

Mahima, Rahal A, Deb R, Latheef SK, Samad HA, Tiwari R, Verma AK, Kumar A and Dhama K. 2012. Immunomodulatory and therauptic potential of herbal, traditional / indigenous and ethanoveterinary medicine. Pakistan Journal of Biological Sciences 15(16): 754–774. DOI: https://doi.org/10.3923/pjbs.2012.754.774

Maqbool I, Wani Z A, Shahardar R A, Allaie I M and Shah M M. 2017. Integrated parasite management with special reference to gastro-intestinal nematodes. Journal of Parasitic Diseases 41(1): 1–8. DOI: https://doi.org/10.1007/s12639-016-0765-6

Marklund S and Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47: 469–474. DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Moudgil A D, Sharma A, Verma M S, Kumar R, Dogra P K and Moudgil P. 2017. Gastrointestinal parasitic infections in Indian Gaddi (goat) breed bucks: Clinical, hemato-biochemical, parasitological and chemotherapeutic studies. Journal of Parasitic Diseases 41(4): 1059–1065. DOI: https://doi.org/10.1007/s12639-017-0934-2

Negash B, Seyoum W and Sheferaw D. 2023. Seasonal prevalence and status of anthelmintic resistance of goats’ gastrointestinal DOI: https://doi.org/10.1155/2023/9945998

nematodes, Mirab Abaya, Southern Ethiopian Journal of Parasitological Research 9945998.

Rahman M, Labony S, Dey A and Alam M Z. 2017. An epidemiological investigation of gastrointestinal parasites of small ruminants in Tangail, Bangladesh. Journal of the Bangladesh Agricultural University 8(3): 163–165. DOI: https://doi.org/10.3329/jbau.v15i2.35071

Ramadan M F and Morsel J T. 2004. Oxidative stability of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and Niger (Guizotia abyssinica Cass.) upon stripping. European Journal of Lipid Science and Technology 106: 35–43. DOI: https://doi.org/10.1002/ejlt.200300895

Ramakant, Sarkar T K, Verma A K, Maurya P S, Jaiswal V, Singh A, Thakur V and Rawat S. 2025. PCR based identification of different strongyle species in goats of western Uttar Pradesh, India. Indian Journal of Animal Sciences 95(1): 13–17. DOI: https://doi.org/10.56093/ijans.v95i1.157162

Ramakant, Sarkar TK, Verma AK, Maurya PS, Thakur V, Singh A, Rawat S, Chandra G, Maurya PK and Gautam S. 2024a. Prevalence of gastrointestinal parasitic infections in goats in Western Uttar Pradesh, India. Acta Scientific Veterinary Sciences 6(6): 17–27. DOI: https://doi.org/10.31080/ASVS.2024.06.0881

Ramakant, Sarkar TK, Verma A K, Maurya P S, Thakur V, Singh A, Jaiswal V, Rawat S, Maurya P K and Gautam S. 2024b. In vitro anthelmintic activity of aqueous extracts of seven medicinal plants against Haemonchus contortus in goats. International Journal of Advanced Biochemistry Research SP-8(6): 51–58 DOI: https://doi.org/10.33545/26174693.2024.v8.i6Sa.1254

Ratanapob N, Thuamsuwan N and Thongyuan S. 2022. Anthelmintic resistance status of goat gastrointestinal nematodes in Sing Buri Province, Thailand. Veterinary World 15(1): 83–90. DOI: https://doi.org/10.14202/vetworld.2022.83-90

Risa D, Mamo G, Waktole H, Haile G and Terefe G. 2024 Goats are more susceptible to Haemonchus contortus infection than sheep under similar experimental settings. Scientific Reports 14(1): 25379. DOI: https://doi.org/10.1038/s41598-024-74112-1

Samojlik I, Lakic N, Mimica-Dukic N, Dakovic-Svajcer K and Bozin B. 2010. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). Journal of Agricultural and Food Chemistry 58: 8848–8853. DOI: https://doi.org/10.1021/jf101645n

Sarkar V K, Kamalla R, Solanki P, Mehra S, De U K, Pateer D P, Saxena H and Sakshi 2024. Analysis of gastrointestinal parasitic impact on goats: Insights into infection rates, hematological and biochemical changes, and oxidative stress responses. International Journal of Advanced Biochemistry Research 8(1): 880–885. DOI: https://doi.org/10.33545/26174693.2024.v8.i1Sl.466

Shin MH, Lee YA and Min DY. 2009. Eosinophil-mediated tissue inflammatory responses in helminth infection. Korean Journal of Parasitology 47 (Suppl): S125–131. DOI: https://doi.org/10.3347/kjp.2009.47.S.S125

Shrimali RG, Patel MD and Patel RM. 2016. Comparative efficacy of anthelmintics and their effects on hemato-biochemical changes in fasciolosis of goats of South Gujarat. Veterinary World 9(5): 524–529. DOI: https://doi.org/10.14202/vetworld.2016.524-529

Sobhani Z, Mohtashami L, Amiri M S, Ramezani M, Emami S A and Gandara J S. 2022. Ethnobotanical and phytochemical aspects of the edible herb Coriandrum sativum L. Journal of Food Science 87: 1386–1422. DOI: https://doi.org/10.1111/1750-3841.16085

Soulsby E J L. 1982. Helminths, arthropods and protozoa of domesticated animals. 7th edn., ELBS, Bailliere Tindall, London pp. 763–778.

Sunder J, Bhattacharya D, Sujatha T, De A K, Chakraborty G, Mayuri S C, Perumal P, Bhowmick S, Alyethodi R R and Chakurkar E B. 2025. Use of FAMACHA to detect anaemia and control of gastrointestinal parasite in goats of A and N Islands, India. Indian Journal of Animal Research 59(3): 503–508.