Biometrical and histomorphological studies on the postnatal ontogenesis of black Bengal goat epididymis
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DOI:
https://doi.org/10.56093/ijans.v96i8.167429Keywords:
Biometry, histomorphometry, epididymis, postnatal development, black Bengal goatAbstract
Background and Objectives: Understanding the postnatal development of the epididymis is crucial for comprehending male fertility. The study designed to describe the baseline biometrical and histomorphometric epididymal changes during postnatal ontogenesis in Black Bengal goats. Materials and Methods: Black Bengal goats (n=21), divided into group I–VII: day 0; 1, 2 weeks; 1, 2, 4, and 6 months of age, respectively, were used in this study. Routine hematoxylin and eosin staining was used for histomorphometric study. Results: The Black Bengal goat epididymis followed a regionalized pattern of development, with the tail beginning development before other anatomical regions. The epididymal development was slow until 1 month of age, and then a rapid epididymal development was observed. The epididymal length and weight were 5 and 40 times greater, respectively, at puberty than those at birth. The epithelial height and tubular diameter in all three regions were significantly increasing from 1 month of age except in tail at 6 months when the epithelial height was drastically reduced. Epididymal regions were significantly (p<0.05) different from one another from 4 months and 6 months in terms of tubular diameter and epithelial height, respectively. Stereocilia were observed from 4 months, and sperm were first appeared in the tubular lumen of all three regions of epididymis by 6 months which indicates that by that age the black Bengal goats had already reached or very close to reach the puberty. Conclusion: This is the first report of detailed description of black Bengal goat epididymis morphometry during postnatal ontogenesis.
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1. Ahmad N and Noakes D. E. 1996. Sexual maturity in British breeds of goat kids. British Veterinary Journal 152(1): 93-103, doi: 10.1016/S0007-1935(96)80089-3.
2. Alam M A, Ershaduzzman M, Khatun R, Talukder M A I and Sarker N R. 2020. Growth and reproductive performances of Hilly Brown Bengal goat under semi-intensive management condition at Naikhongchari hilly areas of Bangladesh. Asian-Australasian Journal of Bioscience and Biotechnology 5(2): 72-77.
3. Arrighi S, Bosi G, Groppetti D and Cremonesi F. 2010. Morpho- and histometric evaluations on the testis and epididymis in buffalo bulls during the different reproductive seasons. Open Anatomy Journal 2: 29-33.
4. Belkhiri Y, Bouzebda–Afri F, Bouzebda Z, Benbia S and Lamraoui R. 2025. Histological analysis of epididymis and testis in the dynamics of postnatal ontogenesis from one month to puberty of Ouled Djellal lambs. Revista Científica de la Facultad de Ciencias Veterinarias 35(1): 9. doi: 10.52973/rcfcv-e35521
5. Bjorkgren I L, Saastamoinen A, Krutskikh I, Huhtaniemi M and Sipila P. 2012. Dicer1 ablation in the mouse epididymis causes dedifferentiation of the epithelium and imbalance in sex steroid signaling. Public Library of Science One 7: e38457. doi: 38410.31371/journal.pone.0038457.
6. Cadena-Villegas S, Hernández-Marín J A, GallegosSánchez J, Germán- Alarcón C G and Pérez-Hernández P. 2021. Reproductive management of the male goat: A review. Agro Productividad. https://doi.org/10.32854/agrop. v14i8.2102
7. Chakraborty S and Saha S. 2000. Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertility Society Journal 27: 4. doi: 10.1186/s43043-022-00094-7
8. Cornwall G A. 2009. New insights into epididymal biology and function. Human Reproduction Update 15: 213-227.
9. Gofur M R and Ogawa K. 2019. Compartments with predominant ephrin-B1 and EphB2/B4 expression are present alternately along the excurrent duct system in the adult mouse testis and epididymis. Andrology 7: 888-901. doi: 10.1111/andr.12523
10. Gofur M R and Ogawa K. 2024. EphB2, EphB4 and ephrin-B1 expression and localization in postnatal developing epididymis in mice. Developmental Dynamics 1‐16. doi: 10.1002/dvdy.752
11. Gofur M R, Khan M Z I, Karim M R and Islam M N. 2008. Histomorphology and histochemistry of testis of indigenous bull (Bos indicus). Bangladesh Journal of Veterinary Medicine 6(1): 67-74.
12. Gofur M R, Rauf S M A, Islam M M, Alam M E and Alam J. 2024. Testis and epididymis of indigenous buffalo bull: A biometrical and histomorphometrical study. Asian Science Bulletin 2(1): 10–16.
13. Gofur M R, Sadi M S, Aktar S, Khatun A, Awal M A, Alam M E, Rauf S A M and Matsuo K. 2023. Biometrical and histomorphometrical changes of testis in the dynamics of postnatal ontogenesis from birth to puberty of Black Bengal goat. Journal of Advanced Veterinary and Animal Research 10(2): 237–243. doi: 10.5455/javar.2023.j674
14. Gofur M R. 2015. Anatomy and histomorphometry of accessory reproductive glands of Black Bengal buck. European Journal of Anatomy 19(2): 171-178.
15. Herrera-Alarcón J, Villagómez-Amezcua E, González-Padilla E and Jiménez-Severiano H. 2007. Stereological study of postnatal testicular development in Blackbelly sheep. Theriogenology 68: 582-591.
16. Hossain M E. 2021. Performance of Black Bengal goat: a 50-year review. Tropical Animal Health and Production 53: 71. doi:10.1007/s11250-020-02477-2
17. James E R, Carrell D T, Aston K I, Jenkins T G, Yeste M and Salas-Huetos A. 2020. The role of the epididymis and the contribution of epididymosomes to mammalian reproduction. International Journal of Molecular Sciences 21(15): 5377. doi: 10.3390/ijms21155377.
18. Kangawa A, Otake M, Enya S, Yoshida T and Shibata M. 2019. Histological changes of the testicular interstitium during postnatal development in microminipigs. Toxicolological Pathology 47(4): 469-482.
19. Kour J, Devi J and Sarma K. 2023. Effect of zinc supplementation on histomorphology of testis and epididymis in male wistar rats. Indian Journal of Animal Research 57(2): 184-189. doi: 10.18805/IJAR.B-4312.
20. Kumaran G K and Hanukoglu I. 2020. Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns. Federation of European Biochemical Societies 287(6): 1176-1194. doi: 10.1111/febs.15088.
21. Mendive F, Laurent P, Van Schoore G, Skarnes W, Pochet R and Vassart G. 2006. Defective postnatal development of the male reproductive tract in LGR4 knockout mice. Developmental Biology 290(2): 421-434. doi: 410.1016/j.ydbio.2005.1011.1043.
22. Meng L J, Wang W Y, Xu Y J, Gong T and Yang Y. 2020. Postnatal differentiation and regional histological variations in the ductus epididymidis of the Congjiang Xiang pig. Tissue and Cell 67: 101411. doi: 10.1016/j.tice.2020.101411.
23. Naik S G, Baddi S Y and Rajashailesha N M. 2023. Gross morphology and histological details of testis and epididymis of the Hassan ram. The Pharma Innovation Journal 12(3): 1514-1518.
24. Pathak A, Katiyar R S, Sharma D N and Farooqui M M. 2014. Postnatal developmental anatomy of testes and epididymis of Gaddi goats. International Journal of Morphology 32(4): 1391-1398.
25. Pilutin A, Misiakiewicz-Has K, Rzeszotek S and Wiszniewska B. 2021. Morphological and morphometric changes and epithelial apoptosis are induced in the rat epididymis by long-term letrozole treatment. European Journal of Histochemistry 65(3): 3259. doi: 10.4081/ejh.2021.3259.
26. Ramukhithi F V, Nephawe K A, Chokoe T C, Matabane M B, Mphaphathi M L, Lehloenya K C and Nedambale T L. 2017. Attainment of puberty in South African unimproved indigenous bucks. Small Ruminant Research 153: 57-61. doi: 10.1016/j.smallrumres.2017.05.009
27. Robaire B and Hamzeh M. 2011. Androgen action in the epididymis. Journal of Andrology 32: 592-599.
28. Rodriguez-Martinez H, Roca J, Alvarez-Rodriguez M and Martinez-Serrano C A. 2022. How does the boar epididymis regulate the emission of fertile spermatozoa? Animal Reproduction Science 246: 106829. doi:10.1016/j.anireprosci.2021.106829
29. Rus M, Groza I, Pall E, Peştean C, Daradics S, Adriana G, Crecan C and Morar I. 2022. Ampullar and ductus deferens aspiration increases the total number of viable spermatozoa recovered from stallion extragonadal sperm reserves. Indian Journal of Animal Research 56(12): 1478-1482. doi: 10.18805/IJAR.BF-1539.
30. Sadi M S and Gofur M R. 2022. Temporal changes in macro- and microstructure and seminiferous epithelium of the indigenous sheep testis from birth to puberty. Asian Journal of Animal and Veterinary Advances 17: 137-145.
31. Sarma L, Nahardeka N, Goswami R N, Aziz A, Zaman G, Das A and Akhtar F. 2019. Non-genetic factors affecting pre-weaning growth and morphometric traits in Assam Hill goat. Veterinary World 12(8): 1327-1331. doi: 10.14202/vetworld.2019.1327-1331
32. Sharma R K, Goyal A K and Yadav V. 2014. Histological studies on epididymis region of goat (Capra hircus) reproductive system. International Journal of Pure and Applied Zoology 2(3): 224-227.
33. Singh N, Sathapathy S, Patra R, Biswal S S, Behera S S, Mishra S R, Mishra P K K, Joshi S K, Sahu R and Swain P S. 2025. Scanning electron microscopic studies on the development of epididymis and vas deferens in non-descript goat (Capra hircus) during postnatal period. Indian Journal of Animal Research 1-8. doi: 10.18805/IJAR.B-5532.
34. Sullivan R and Mieusset R. 2016. The human epididymis: its function in sperm maturation. Human Reproduction Update 22(5): 574–587.
35. Vargas-Velázquez A D and Jiménez-Severiano H. 2016. Morphometric study of the epididymes during the postnatal development in Barbados Blackbelly ram lambs. Revista Mexicana de Ciencias Pecuarias 7(1): 53-68.
36. Wong J, Gasperoni J, Fuller J, Grommen S V H, De Groef B, Hogarth C and Dworkin S. 2022. Crucial convolution: Genetic and molecular mechanisms of coiling during epididymis formation and development in embryogenesis. Journal of Developmental Biology 10(2): 25. doi: 10.3390/jdb10020025
37. Zayed A E, Aly K, Ibrahim I A and Abd El-Maksoud F M. 2012. Morphological studies on the epididymal duct of the one-humped camel (Camelus dromedaries). Open Journal of Veterinary Medicine 2(4): 245-254.
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