Seasonal histomorphological studies on acidophils of hypophysis cerebri of Gaddi goats in relation to climate change

ARJUN

doi:10.56093/ijans.v95i7.168240

Authors

ARJUN LALA LAJPAT RAI UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES HISAR HARYANA https://orcid.org/0009-0005-1151-6322

https://doi.org/10.56093/ijans.v95i7.168240

Keywords:

Gaddi goat, pituitary gland, climate change, acidophils, seasonal variation, reproduction, histomorphology

Abstract

The present study was conducted to investigate the seasonal histomorphological variations in acidophil cells of the pituitary gland in Gaddi goats, with reference to changing climatic conditions. A total of 35 heads from adult, pluriparous, non-pregnant Gaddi does were procured from local slaughterhouses in the Palampur region of Himachal Pradesh during different seasons. The pituitary gland, located in the interpeduncular fossa, appeared as a light grey, oval or pyriform structure. Histological analysis of the pars distalis adenohypophysis revealed significant seasonal differences (P<0.05) in the relative proportions of acidophils, abundant during the monsoon season. These were further classified into somatotrophs (orangeophils) and lactotrophs (carminophils) using Crossman’s modification of Mallory’s trichome stain, both showing minimal to nill PAS reactivity. The average diameter of somatotrophs (STH-ocytes) was largest in the monsoon (11.4±0.20 µm) and smallest in winter (10.01±0.33 µm). Similarly, lactootrophs (LTH-ocytes) showed maximum diameter during the monsoon (10.6±0.14 µm) and minimum in spring (8.41±0.22 µm). A comparison with the previous seasonal histomorphological studies revealed a shift in cellular dimensions of acidophils over time, indicating that the relative increase in temperature and humidity over the past 20 years may be impacting the migration patterns and pituitary functions in Gaddi goats, with potential effects on their reproductive performance and productivity

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References

Chakravarthy, Y.S. and Mariappa, D. (1975). Histological observations on the pituitary of Indian buffalo (Bos bubalis). Indian Veterinary Journal, 52: 121–124.

Crossman, G.A. (1937). A modification of Mallory's connective tissue stain with discussion of principles involved. Anatomical Record, 69: 33–38.

Ganong, W.F. (2005). Review of medical physiology. 22nd edn. McGraw-Hill, New York.

Gebbie, F.E., Forsyth, I.A. and Arendt, J. (1999). Effects of maintaining solstice light and temperature on reproductive activity, coat growth, plasma prolactin and melatonin in goats. Journal of Reproduction and Fertility, 116(1): 25–33.

Herlant, M. and Racadot, S. (1957). Lobe antérieur de l’hypophyse de la chatte au cours de la gestation de la lactation. Archives de Biologie, 11: 217–248.

IPCC. (2021). Sixth Assessment Report: Climate change 2021 – The physical science basis. Intergovernmental Panel on Climate Change, Geneva.

Jubb, K.V. and McEntee, K. (1955). Observation on the bovine pituitary gland. II. Architectural and cytology with special reference to basophil cell function. Cornell Veterinarian, 45: 593–641.

Khan, M., Saigal, R.P. and Sethi, R.S. (1998). Somatotrophs of buffalo – A histomorphological and immunohistochemical study. Indian Journal of Animal Sciences, 68: 1221–1223.

Lincoln, G.A., Almeida, O.F.X. and Fraser, H.M. (1996). Photoperiod–melatonin interactions in seasonal prolactin secretion. Journal of Neuroendocrinology, 8(5): 457–464.

Luna, L.G. (1968). Manual of histological staining methods. McGraw-Hill Book Co., New York.

Mallory, F.B. (1961). Pathological technique. Hafner Publishing Co., New York, p. 152.

Misztal, T., Romanowicz, K. and Barcikowski, B. (1997). Natural and melatonin-stimulated changes in the circadian rhythm of prolactin secretion in the ewe during seasonal anestrus. Neuroendocrinology, 66: 360–367.

Nishimura, S., Ikeda, K., Nakayama, M., Okano, K. and Iwamoto, H. (1998). Regional distribution and ultrastructural characterisation of growth hormone secreting cells in the adenohypophysis of Tokara goat. Journal of the Faculty of Agriculture, Kyushu University, 43: 345–354.

Noden, D.M. and de Lahunta, A. (1985). The embryology of domestic animals. Williams and Wilkins, Baltimore, p. 673.

Paramasivan, S. (2000). Histological and histochemical studies on hypothalamus, pituitary and ovary of ewe (Gaddi ewe). M.V.Sc. thesis, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishwavidyalaya, Palampur, India.

Pathak, V. (2001). Correlative anatomical studies on the hypothalamo-hypophysio-gonadal axis of Gaddi doe. M.V.Sc. thesis, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishwavidyalaya, Palampur, India.

Pathak, V. (2006). Regional distribution of various cell types in the pars distalis adenohypophysis of Gaddi goats. Indian Journal of Animal Sciences, 76(9): 211–214 .

Pearse, A.G.E. (1952). The cytochemistry and cytology of normal anterior hypophysis investigated by trichrome PAS method. Journal of Pathology, 64: 811–826.

Prasad, G. and Singh, L.P. (1980). Histological and certain histochemical studies on the adenohypophysis of the non-pregnant Indian buffalo (Bubalus bubalis). Indian Journal of Animal Sciences, 50: 710–717.

Ricci, V. and Russola, N. (1973). Immunocytological observations on the localization of ACTH in the hypophysis of the dog. Acta Anatomica, 84: 10–18.

Roy, M.K. (1970). Histology and certain histochemical studies and the endocrine glands of buffalo. M.V.Sc. thesis, Agra University, India.

Sejian, V., Maurya, V.P. and Naqvi, S.M.K. (2010). Adaptability and growth of Malpura ewes subjected to thermal and nutritional stress. Tropical Animal Health and Production, 42(8): 1763–1770.

Sejian, V., Maurya, V.P. and Naqvi, S.M.K. (2018). Adaptation strategies in small ruminants against climate change: An overview. Indian Journal of Animal Sciences, 88(3): 285–297.

Sejian, V., Maurya, V.P., Kumar, K. and Naqvi, S.M.K. (2013). Effect of multiple stresses (thermal, nutritional and walking stress) on the reproductive performance of Malpura ewes. Veterinary Medicine International, 2012: Article ID 471760.

Singh, G., Dhingra, L.D. and Singh, Y. (1991). Histomorphology of pars distalis adenohypophysis of sheep during different stages of pregnancy. Indian Journal of Animal Sciences, 61: 792–797.

Tassel, R. and Kennedy, J.P. (1972). Early follicular development and atretic changes in the ovary of the lamb – Fine structure and histochemistry. Australian Journal of Biological Sciences, 33: 675–687.