Detection of Leptospira spp. in animals and environmental contaminantby dark field microscopy, real-time PCR, microscopic agglutination testand enzyme linked immunosorbent assay
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Authors
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ATUL H NEHETE
Maharashtra Animal and Fishery Sciences University, Mumbai, Maharashtra 400 012 India
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VILAS M VAIDYA
Maharashtra Animal and Fishery Sciences University, Mumbai, Maharashtra 400 012 India
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RAVINDRA J. ZENDE
Maharashtra Animal and Fishery Sciences University, Mumbai, Maharashtra 400 012 India
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RAJASHREE S GANDGE
Maharashtra Animal and Fishery Sciences University, Mumbai, Maharashtra 400 012 India
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RAJPAL S KHILLARE
Maharashtra Animal and Fishery Sciences University, Mumbai, Maharashtra 400 012 India
Keywords:
DFM, ELISA, Leptospira, MAT, RT-PCRAbstract
The present study demonstrated endemic circulation of pathogenic Leptospira spp. among animals and environmental water sources in the Mumbai region. RTPCR assay targeting the lipL32 gene proved significantly more sensitive than DFM for detection of active infection (P<0.001), while MAT and ELISA revealed higher seropositivity reflecting previous exposure and circulation of leptospires in the region. Dogs showed the highest seropositivity, with Pyrogenes and Shermani emerging as predominant serovars, whereas Pomona and Tarassovi predominated in bovines. The detection of pathogenic leptospires in environmental water samples highlighted
the important role of environmental reservoirs in disease transmission. The combined use of molecular and serological assays provided a comprehensive understanding of leptospirosis epidemiology and supports continuous surveillance, inclusion of locally prevalent serovars in vaccine formulations and implementation of integrated One Health strategies for effective disease control.
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References
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