Association between PROP1 gene polymorphism (g.748G>A) and growth traits in Assam hill goats

RAKESH  KUMAR; DIPAK  BHUYAN; MAHAK  SINGH; MEENA  DAS; RAHUL  KATIYAR; P C  CHANDRAN; R K  KAMAL; SHANKAR  DAYAL; RAJNI  KUMARI; AMITAVA  DEY; P K  RAY; JYOTI  KUMAR; S K  AHIRWAL; KAMAL  SARMA; ANUP  DAS

doi:10.56093/ijans.v96i7.168972

Authors

RAKESH KUMAR ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
DIPAK BHUYAN Assam Agricultural University, Goat Research Station, Byrnihat-793 101, Assam
MAHAK SINGH Assam Agricultural University, Goat Research Station, Byrnihat-793 101, Assam
MEENA DAS ICAR- Research Complex for North Eastern Hilly (NEH) Region, Nagaland Centre 797 106, Nagaland
RAHUL KATIYAR ICAR- Research Complex for North Eastern Hilly (NEH) Region, Umiam-793 103, Meghalaya
P C CHANDRAN ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
R K KAMAL ICAR-RCER, Farming System Research Centre for Hill and Plateau Region (FSRCHPR) Plandu, Ranchi-834 010, Jharkhand
SHANKAR DAYAL ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
RAJNI KUMARI ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
AMITAVA DEY ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
P K RAY ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
JYOTI KUMAR ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
S K AHIRWAL ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
KAMAL SARMA ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India
ANUP DAS ICAR-Research Complex for Eastern Region (RCER), Patna 800 014 Bihar India

https://doi.org/10.56093/ijans.v96i7.168972

Keywords:

Assam Hill goat, Growth traits, PCR-RFLP, Polymorphism, PROP1 gene

Abstract

PROP1 (Prophet of Pit-1) gene is a key transcription factor involved in pituitary development and regulation of growth-related hormones in livestock. This study investigated polymorphisms in the PROP1 gene and their association with growth traits in Assam Hill goats. Genomic DNA was extracted from blood samples collected from 147 unrelated Assam Hill goats. Primers targeting intron 1 and exon 1 of the PROP1 gene were designed using Primer3Plus, and genotyping was performed by PCR–RFLP. Population genetic parameters were estimated using PopGene version 1.32. A missense SNP, g.748G>A, was identified and genotyped using the restriction enzyme DrdI, which revealed three genotypes (GG, GA, and AA). The frequencies of alleles A and G were 0.53 and 0.47, respectively. The locus exhibited moderate polymorphism (PIC = 0.37) and moderate genetic diversity, with an effective number of alleles of 1.99 and an expected heterozygosity of 0.50. Association analysis revealed that animals carrying AA genotype tended to exhibit superior growth performance, with significantly greater body length and wither height at 6 months of age than those carrying the GG and GA genotypes (P < 0.05). These findings suggest that the PROP1 g.748G>A polymorphism might serve as a potential molecular marker for marker-assisted selection aimed at improving growth traits in Assam Hill goats. However, further validation in larger, independent, and genetically diverse goat populations is required before its practical application in breeding programmes.

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