Influence of dietary L-threonine on egg production, egg quality,immune functions, gut health and anti-oxidant status in laying hens

BIKAS CHANDRA  DEBNATH; PURNENDU  BISWAS; BARUN  ROY; MANIK CHANDRA  PAKHIRA; JOYDIP  MUKHERJEE; NARESH  KURECHIYA

doi:10.56093/ijans.v96i1.169810

Authors

BIKAS CHANDRA DEBNATH College of Veterinary Sciences and A.H, R.K. Nagar, Tripura, India
PURNENDU BISWAS West Bengal University of Animal and Fishery Sciences, Kolkata 700 037 India
BARUN ROY West Bengal University of Animal and Fishery Sciences, Kolkata 700 037 India
MANIK CHANDRA PAKHIRA West Bengal University of Animal and Fishery Sciences, Kolkata 700 037 India
JOYDIP MUKHERJEE West Bengal University of Animal and Fishery Sciences, Kolkata 700 037 India
NARESH KURECHIYA West Bengal University of Animal and Fishery Sciences, Kolkata 700 037 India

https://doi.org/10.56093/ijans.v96i1.169810

Keywords:

Albumin index, Egg mass, Haugh unit, Threonine, Villus height

Abstract

This study was conducted on 1680 BV-300 layers, aged 40 weeks (1352±5.85g) divided into five groups (336 birds each) fed on various levels of dietary L-threonine in their diets: T1 (100% threonine according to NRC recommendations), T2 (100% threonine based on BV-300 requirements), T3 (110% threonine of BV-300 requirements), T4 (120% threonine of BV-300 requirements) and T5 (130% threonine of BV-300 requirements), over a period of twenty weeks. Feed efficiency improved in T4 and T5 groups as compared to T1 group. Egg mass was higher in T3, T4 and T5 groups as compared to T1 and T2 groups. Albumin height, albumin index and Haugh Unit exhibited higher values in T4 group than T1 and T2 groups. The total immunoglobulin level was elevated in T3, T4 and T5 groups. The ND titre (HA-HI) was higher in T5 group than T1 and T2 group. The proliferation response index of lymphocyte was higher in the T5 group than the T1 group. The phagocytic activity index of neutrophil was superior in T5 group than T1 and T2 groups. Villus height was higher and villus surface area and the goblet cells number/villus were greater in T4 and T5 group than in T2 group. The T5 group exhibited a higher concentration of GSH-Px as compared to T1 group. Additionally, T5 group demonstrated a higher level of SOD activity than the
other groups. The best relative economic efficiency was recorded in T4 group. It can be concluded that dietary threonine level may be increased by 20% and 30% of BV-300 requirement for optimal performance and immune health, respectively in layers.

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References

Abbasi M A, Mahdavi A H, Samie A H and Jahanian R. 2014. Effects of different levels of dietary crude protein and threonine on performance, humoral immune responses and intestinal morphology of broiler chicks. Brazilian Journal of Poultry Science 16 (1): 35–44. DOI: https://doi.org/10.1590/S1516-635X2014000100005

Abdel-Wareth A A A and Esmail Z S H. 2014. Some productive egg quality and serum metabolic profile responses due to L-threonine supplementation to laying hen diets. Asian Journal of Poultry Science 8: 75–81. DOI: https://doi.org/10.3923/ajpsaj.2014.75.81

A O A C International. 1995. Association of Analytical Chemists. Official Methods of Analysis. 16th ed. AOAC Int., Arlington, USA, pp. 4.1–4.17.

Azzam M M M, Dong X Y, Xie P, Wang C and Zou X T. 2011. The effect of supplemental L-threonine on laying performance, serum free amino acids, and immune function of laying hens under high-temperature and high-humidity environmental climates. Journal of Applied Poultry Research 20: 361–370. DOI: https://doi.org/10.3382/japr.2010-00308

Azzam M M M, Dong X Y, Xie P and Zou X T. 2012. Influence of L-throenine supplementation on goblet cell numbers, histological structure and antioxidant enzyme activities of laying hens reared in a hot and humid climate. British Poultry Science 53: 640–645. DOI: https://doi.org/10.1080/00071668.2012.726707

Azzam M M M, Dong X Y and Zou X T. 2016. Effect of dietary threonine on laying performance and intestinal immunity of laying hens fed low-crude-protein diets during the peak production period. Journal of Animal Physiology and Animal Nutrition 101(5): e55-e66. DOI: https://doi.org/10.1111/jpn.12559

Azzam M M M, Yuan C, Liu GH and Zou X T. 2014. Effect of excess dietary threonine on laying performance, egg quality, serum free amino acids, and digestive enzymes activities of laying hens during the post-peak period. Journal of Applied Poultry Research 23: 605–611. DOI: https://doi.org/10.3382/japr.2013-00913

Bertechini AG 2012 Nutrição de monogástricos, 2ª ed. Lavras, UFLA Bhargava K K, Hanson R P and Sunde M L. 1971. Effects of

threonine on growth and antibody production in chicks infected with Newcastle disease virus. Poultry Science 50: 710–713. DOI: https://doi.org/10.3382/ps.0500710

Burkholder K M, Thompson K L, Einstein M E Applegate T J and Patterson J A. 2008. Influence of stressors on normal intestinal

microbiota, intestinal morphology, and susceptibility to Salmonella enteritidis colonization in broilers. Poultry Science 87: 1734–1741. DOI: https://doi.org/10.3382/ps.2008-00107

Debnath B C, Biswas P and Roy B. 2019. The effects of supplemental threonine on performance, carcass characteristics, immune response and gut health of broilers in sub-tropics during pre-starter and starter period. Journal of Animal Physiology and Animal Nutrition 103 (1): 29–40. DOI: https://doi.org/10.1111/jpn.12991

Dong X Y, Azzam M M M and Zou X T. 2017. Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens. Poultry Science 96 (10): 3654–3663. DOI: https://doi.org/10.3382/ps/pex185

Duval D, Demangel C, Munierjolain K, Miossec S, Geahel I. 1991. Factors controlling cell proliferation and antibody production in mouse hybridoma cells 1 Influence of the amino acid supply. Biotechnology and Bioengineering 38: 561–570. DOI: https://doi.org/10.1002/bit.260380602

Fouad A M, Zhang H X, Chen W, Xia W G, Ruan D, Wang S and Zheng C T. 2017 Estimation of L-threonine requirements for Longyan laying ducks. Asian-Australasian Journal of Animal Science 30 (2): 206–210. DOI: https://doi.org/10.5713/ajas.16.0235

Gawade L, Dadarkar S S, Husain R and Gatne M. 2013. A detailed study of developmental immune-toxicity of imidacloprid in Wistar rats. Food and Chemical Toxicology 51: 61–70. DOI: https://doi.org/10.1016/j.fct.2012.09.009

Geyra A, Uni Z and Sklan D. 2001. The effect of fasting at different ages on growth and tissues dynamics in the small intestine of DOI: https://doi.org/10.1079/BJN2001368

the young chick. British Journal Nutrition 86(1): 53–61.

Gheisar M M, Foroudi F and Shad A G. 2011. Effect of using L–threonine and reducing dietary levels of crude protein on egg production in layers. Iranian Journal of Applied Animal Science 1(1): 65–68.

Habte-Tsion H M, Ren M, Liu B, Ge X, Xie J and Chen R. 2016. Threonine modulates immune response, antioxidant status and gene expressions of antioxidant enzymes and antioxidantimmune-cytokine-related signaling molecules in juvenile blunt snout bream (Megalobrama amblycephala). Fish Shellfish Immunology 51: 189–199. DOI: https://doi.org/10.1016/j.fsi.2015.11.033

Horn N L, Donkin S S, Applegate T J and Adeola O. 2009. Intestinal mucin dynamics: Response of broiler chicks and White Pekin ducklings to dietary threonine. Poultry Science 88: 1906–1914. DOI: https://doi.org/10.3382/ps.2009-00009

Hossaninejad S A, Zarghi H and Golian A. 2021. Effect of digestible threonine on performance, egg quality, blood metabolites, and immune responses in laying hens fed a wheat-based diet in the second cycle. Italian Journal of Animal Science 20 (1): 2134–2146. DOI: https://doi.org/10.1080/1828051X.2021.2004248

Jahanian R. 2010. Threonine needs of growing broiler chickens for performance and optimum immunological functions in response to dietary crude protein concentration. 2nd International Veterinary Poultry Congress, 20-21, Tehran. Iran. p. 200.

Khan A R, Nawaz H and Zahoor I 2006. Effect of different levels of digestible threonine on growth performance of broiler chicks. Journal of Animal and Poultry Production 16: 1–2.

Khan M, Khose K, Manwar S, Gole M, Ali S and Siddiqui F. 2018. Effect of L-Threonine supplementation in diets on immune response and carcass traits in broiler chickens. International Journal of Livestock Research 8(7): 335–341. DOI: https://doi.org/10.5455/ijlr.20171031090856

Kidd M T, Corzo A, Hoehler D, Kerr B J, Barber S J and Branton S L. 2004. Threonine needs of broiler chickens with different growth rates. Poultry Science 83: 1368–1375. DOI: https://doi.org/10.1093/ps/83.8.1368

Kim S W, Mateo R D, Yin Y L and Wu G. 2007. Functional amino acids and fatty acids for enhancing production performance of sows and piglets. Asian Australasian Journal of Animal Science 20: 295–306. DOI: https://doi.org/10.5713/ajas.2007.295

Larbier Z M, Chaqneau A M and Geraert P A. 1993. Influence of ambient temperature on true digestibility of protein and amino acids of rapeseed and soybean meals in broilers. Poultry Science 72: 289–295. DOI: https://doi.org/10.3382/ps.0720289

Law G K, Bertolo R F, Adjiri-Awere A, Pencharz P B and Ball R O. 2007. Adequate oral threonine is critical for mucin production and gut function in neonatal piglets. American Journal of Gastrointestinal and Liver Physiology 292: 1293–1301. DOI: https://doi.org/10.1152/ajpgi.00221.2006

Li P, Yin Y L, Li D, Kim S W and Wu G. 2007. Amino acids and immune function: a review. British Journal of Nutrition 98: 237–252. DOI: https://doi.org/10.1017/S000711450769936X

MacDuogall E D and Klasing K C. 1998. The effect of dietary threonine supplementation on growth and the immunocompetence in chicks. The Federation of American Societies for Experimental Biology Journal12: A873.

Mahmoud K Z and Yaseen A M. 2005. Effect of feed withdrawal and heat acclimatization on stress responses of male broiler and layer-type chickens (Gallus gallus domesticus). Asian Australasian Journal Animal Science 18: 1445–1450. DOI: https://doi.org/10.5713/ajas.2005.1445

Mashaly M M, Hendricks GL, Kalama MA, Gehad A E, Abbas A O, Patterson P H. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poultry Science 83: 889–894. DOI: https://doi.org/10.1093/ps/83.6.889

Min Y, Liu S, Qu Z, Meng G and Gao Y. 2017. Effects of dietary threonine levels on growth performance, serum biochemical indexes, antioxidant capacities and gut morphology in broiler chickens. Poultry Science 96:1290–1297. DOI: https://doi.org/10.3382/ps/pew393

Montagne L, Toullec R and Lalles J P. 2000. Calf intestinal mucin: Isolation, partial characterization, and measurement in ileal digesta with an enzyme-linked immuno-sorbent assay. Journal of Dairy Science 83: 507–517. DOI: https://doi.org/10.3168/jds.S0022-0302(00)74910-1

National Research Council. 1994. Nutrient Requirements of Poultry. 9th rev. ed. National Academy Press. Washington, DC. Nichols N L and Bertolo R F. 2008. Luminal threonine concentration acutely affects intestinal mucosal protein and mucin synthesis in piglets. Journal of Nutrition 1378: 1298–1301.

Rezeipour V, Fononi H and Irani M. 2012. Effects of dietary L-threonine Saccharomyces cerevisiae on performance, intestinal morphology and immune response of broiler chickens. South African Journal of Animal Science 42: 266–273. DOI: https://doi.org/10.4314/sajas.v42i3.8

Sá L M, Gomes P C and Cecon P R. 2007. Exigência nutricional de lisina digestível para galinhas poedeiras no período de 34 a 50 semanas de idade. Revista Brasileira de Zootecnia 36: 1829–1836. DOI: https://doi.org/10.1590/S1516-35982007000800016

Sandberg F B, Emmans G C and Kyriazakis I. 2007. The effects of pathogen challenges on the performance of native and immune animals: the problem of prediction. Animal 1: 67–86. DOI: https://doi.org/10.1017/S175173110765784X

Sandikci M, Eren U, Onol A G and Kum S. 2004. The effect of heat stress the use of Saccharomyces cerevisiae or (and) bacitracin zinc against heat stress on the intestinal mucosa in quails. Revue de Me´decine Ve´te´rinaire 155: 552–556.

Schaart M, Schierbeek W H, Van Der Schoor S R, Stoll B, Burrin DG, Reeds P and Van Goudoever J B. 2005. Threonine utilization is high in the intestine of piglets. Journal of Nutrition 135: 765–770. DOI: https://doi.org/10.1093/jn/135.4.765

Snedecor G W and Cochran W G. 1994. Statistical Methods. 8th ed. Iowa State University Press, Ames, Iowa.

SPSS Inc. (2002) SPSS for Windows: Release 11.5, Standard Version, 1989–2002. SPSS Inc., Chicago, IL.

Wang X, Qiao S Y, Liu M and Ma Y X. 2006. Effects of graded levels of true ileal digestible threonine on performance, serum parameters and immune function of 10–25 kg pigs. Animal Feed Science and Technology 129: 264–278. DOI: https://doi.org/10.1016/j.anifeedsci.2006.01.003

Wu G. 1998. Intestinal mucosal amino acid catabolism. Journal of Nutrition 128: 1249–1252. DOI: https://doi.org/10.1093/jn/128.8.1249

Zaefaria F, Zaghari M and Shivazad M. 2008. The threonine requirements and its effects on growth performance and gut morphology of broiler chicken fed different levels of protein. International Journal of Poultry Science 7: 1207–1215. DOI: https://doi.org/10.3923/ijps.2008.1207.1215

Zaghari M, Zaefarian F and Shivazad, M. 2011. Standardized ileal digestible threonine requirements and its effects on performance and gut morphology of broiler chicks fed two levels of protein. Journal of Agricultural Science and Technology 13: 541–552.