Influence of dietary L-threonine on egg production, egg quality, immune functions, gut health and anti-oxidant status in laying hens

Bikas Chandra Debnath; Dr. Purnendu Biswas; Dr Barun Roy; Dr. Manik Pakhira; Dr. Joydip Mukherjee; Dr. Naresh Kurechiya

doi:10.56093/ijans.v96i1.169810

Authors

Bikas Chandra Debnath COLLEGE OF VETERINARY SCIENCES AND A.H, R.K.NAGAR, TRIPURA
Dr. Purnendu Biswas
Dr Barun Roy
Dr. Manik Pakhira
Dr. Joydip Mukherjee
Dr. Naresh Kurechiya

https://doi.org/10.56093/ijans.v96i1.169810

Keywords:

Albumin index, Egg mass, Haugh unit, Threonine, Villus height

Abstract

This study was aimed to explore the egg production, egg quality, immune responses and gut health of layer birds. Threonine, a third limiting amino acid in poultry nutrition, serves as a key component of mucin and immunoglobulin. 1680 nos. BV-300 layers, aged 40 weeks (1352±5.85g), were divided into five groups (336 birds each) based on various levels of dietary L-threonine (L-Thr) in their diets: 100% Thr according to NRC recommendations, 100% Thr based on BV-300 requirements, 110% Thr of BV-300 requirements, 120% Thr of BV-300 requirements and 130% Thr of BV-300 requirements, over a period of twenty weeks. Feed consumption and cumulative feed conversion ratio (CFCR) showed improvement. Average Hen Day Egg Production (HDEP) exhibited a linear increase, while egg mass (EM) demonstrated both linear and quadratic increment. Significant increase was observed in albumin height (AH), albumin index (AI), and Haugh Unit (HU). Total immunoglobulin levels, HA-HI ND titers, and proliferation response index of lymphocyte (LPR) improved linearly, while neutrophil’s phagocytic activity index showed both linear and quadratic increment. The villus height (VH), villus surface area (VSA) and number of goblet cells per villus increased linearly as well as quadratically, while villus width (VW) and crypt depth (CD) increased linearly. The GSH-Px activity and serum SOD levels rose linearly. The best relative economic efficiency (REE) was recorded in the 120% Thr group. It can be concluded that a recommended dosage of 120% L-Thr (relative to BV-300 requirements) is necessary for optimal performance in layers.

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