Melatonin in cryodiluent improves post-thaw quality andfertility of Murrah buffalo (Bubalus bubalis) bull spermatozoa

P  SINGH; A K  SINGH; P  DEVI; A  KUMAR; N  SINGH; N S  RATTA; P  SINGH

doi:10.56093/ijans.v96i1.170042

Authors

P SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
A K SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
P DEVI Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
A KUMAR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
N SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
N S RATTA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India
P SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab 141 004 India

https://doi.org/10.56093/ijans.v96i1.170042

Keywords:

Buffalo bull semen, Cryopreservation, FSCR, Lipid peroxidation, Melatonin, Sperm attributes

Abstract

The current research aimed to analyze the role of melatonin supplementation (0, 0.25, 0.5, 0.75 and 1.0 mM) on the freezability of buffalo bull spermatozoa. Thirty-six ejaculates were collected from six healthy bulls using artificial vagina. Only ejaculates with ≥70% progressive motility, ≤20% morphological defects and >500 million/ mL sperm concentration were selected for the study. Each ejaculate was diluted in Tris-egg yolk cryodiluent with selected concentrations of melatonin along with control and cryopreserved following established protocol. The post-thaw semen was assessed for sperm attributes and lipid peroxidation (LPO). IBM SPSS 22.0 software was used for statistical evaluations. The sperm total and progressive motility in post-thaw semen were evaluated using computer assisted semen analyzer (CASA) and were higher (P<0.05) in freezing media having 0.25 mM melatonin in comparison to other concentrations. The sperm viability and acrosome integrity were higher (P<0.05) at 0.25 mM melatonin concentration than at 0.75 mM, 1.0 mM and control. The sperm plasma membrane integrity among the experimental extenders supplemented with melatonin and control did not differ (P>0.05). All melatonin concentrations exhibited lower (P<0.05) MDA levels compared to control. The first service conception rate (FSCR) was higher (P>0.05) following inseminations using semen containing 0.25 mM melatonin than in control. Overall, the addition of 0.25 mM melatonin to the cryodiluent improved post-thaw semen quality of Murrah buffalo bulls.

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References

Allegra M, Reiter R, Tan D, Gentile C, Tesoriere L and Livrea M. 2002. The chemistry of melatonin’s interaction with reactive species. Journal of Pineal Research 34(1): 1–10.

Andrabi S, Ansari, Ullah N, Anwar M, Mehmood A and Akhter S. 2008. Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reproduction Science 104(2–4): 427–433.

Ashrafi I, Kohram H and Ardabili F F. 2013. Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa. Animal Reproduction Science 139(1–4): 25–30.

Asma-Ul-Husna A, Ansari M S, Rakha B A, Ejaz R, Ullah N and Akhter S. 2017. Melatonin supplementation in extender enhances the post thaw quality of Buffalo bull spermatozoa. Pakistan Journal of Zoology 49(1): 163–167.

Buege J A and Steven A D. 1978. Microsomal lipid peroxidation. Method of Enzymology 52(30): 302–310.

Chaithra Shree A R, Ingole S D, Dighe V D, Nagvekar A S, Bharucha S V, Dagli N R, Kekan P M and Kharde S D. 2020. Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation. Veterinary Medicine and Science 6(2): 177–186.

Dai G, Meng Y, Zhang L, Du Y, Wen F, Feng T and Hu J. 2019. Effect of addition of melatonin on liquid storage of ram semen at 4°C. Andrologia 51(5): e13236.

Dalton J C, Nadir S, Noftsinger M and Saacke R G. 2010. Insemination related factors affecting fertilization in estrus synchronized cattle. In: Proceedings of Applied Reproductive Strategies in Beef Cattle, San Antonio, pp. 28–29.

Deng S, Sun T, Yu K, Wang Z, Zhang B, Zhang Y, Wang X, Lian Z and Liu Y. 2017. Melatonin reduces oxidative damage and upregulates heat shock protein 90 expression in cryopreserved human semen. Free Radical Biology and Medicine 113: 347–354.

El-Raey M, Badar M A, Assi M M and Rawash Z M. 2015. Effect of melatonin on buffalo bull sperm freezability, ultrastructure changes and fertilizing potentials. Assiut Veterinary Medical Journal 61(144): 201–208.

Fayez E, Samir H, El Sayed, Mohammed A, Rawash Z M and Salama A. 2023. Melatonin supplementation to the freezing extender improves the quality of canine epididymal spermatozoa. Andrologia 1–8.

Fujinoki M. 2008. Melatonin-enhanced hyperactivation of hamster sperm. Reproduction 136(5): 533–541.

García J J, López‐Pingarrón L, Almeida-Souza P, Tres A, Escudero P, García‐Gil F A, Tan D, Reiter R J, Ramírez J M and Bernal‐Pérez M. 2014. Protective effects of melatonin in reducing oxidative stress and in preserving the fluidity of biological membranes: A review. Journal of Pineal Research 56(3): 225–237.

Guthrie H D and Welch G R. 2005. Impact of storage prior to cryopreservation on plasma membrane function and fertility of boar sperm. Theriogenology 63(2): 396–410.

Hardeland R, Tan D X and Reiter R J. 2009. Kynuramines, metabolites of melatonin and other indoles: the resurrection of an almost forgotten class of biogenic amines. Journal of Pineal Research 47(2): 109–126.

Hu J, Zhao X, Tian W, Zan L and Li Q. 2011. Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation. Animal 5(1): 107–112.

Inyawilert W, Rungruangsak J, Liao Y, Tang P and Paungsukpaibool V. 2020. Melatonin supplementation improved cryopreserved

Thai swamp buffalo semen. Reproduction in Domestic Animals 56(1): 83–88.

Jang H, Kim Y, Kim B, Park I, Cheong H, Kim J, Park C, Kong H, Lee H and Yang B. 2010. Ameliorative effects of melatonin against hydrogen peroxide-induced oxidative stress on boar sperm characteristics and subsequent in vitro embryo development. Reproduction in Domestic Animals 45(6): 943–950.

Kathiravan P, Kalatharan J, Karthikeya G, Rengarajan K and Kadirvel. 2011. Objective sperm motion analysis to assess dairy bull fertility using computer-aided system-A review. Reproduction in Domestic Animals 46(1): 165–172.

Medeiros C, Forell F, Oliveira A and Rodrigues J. 2002. Current status of sperm cryopreservation: why isn’t it better? Theriogenology 57(1): 327–344.

Medrano A, Contreras C F B, Herrera F M and Alcantar-Rodriguez A M. 2017. Melatonin as an antioxidant preserving sperm from domestic animals. Asian Pacific Journal of Reproduction 6(6): 241–246.

Memon A A, Wahid H, Rosnina Y, Goh Y, Ebrahimi M and Nadia F. 2012. Effect of antioxidants on post thaw microscopic, oxidative stress parameter and fertility of Boer goat spermatozoa in Tris egg yolk glycerol extender. Animal Reproduction Science 136(1–2): 55–60.

Nair S J, Brar A, Ahuja C, Sangha S and Chaudhary K. 2006. A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Animal Reproduction Science 96(1–2): 21–29.

Nichi M, Bols P, Züge R, Barnabe V, Goovaerts I, Barnabe R and Cortada C. 2006. Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology 66(4): 822–828.

Okatani Y, Wakatsuki A and Kaneda C. 2000. Melatonin increases activities of glutathione peroxidase and superoxide dismutase in fetal rat brain. Journal of Pineal Research 28: 89–96.

Özgür M E, Ulu A, Noma S A, Özcan İ, Balcıoğlu S, Ateş B and Köytepe S. 2020. Melatonin protects sperm cells of Capoeta trutta from toxicity of titanium dioxide nanoparticles. Environmental Science and Pollution Research 27(15): 17843–17853.

Perumal P, Vupru K and Khate K. 2013. Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen. International Journal of Zoology 1–10.

Prasad J K, Kumar S, Mohan G, Shanker U and Agarwal S K. 1999. Hypoosmotic swelling test (HOST) and its response in fresh and freezethawed semen. The Indian Journal of Animal Sciences 69(10): 766–769.

Ramadan T, Taha T, Samak M and Hassan A. 2009. Effectiveness of exposure to long day followed by melatonin treatment on semen characteristics of Damascus male goats during breeding and non-breeding seasons. Theriogenology 71(3): 458–468.

Reiter R J, Paredes S D, Manchester L C and Tan D. 2009. Reducing oxidative/nitrosative stress: a newly-discovered genre for melatonin. Critical Reviews in Biochemistry and Molecular Biology 44(4): 175–200.

Reiter R J, Tan D, Osuna C, and Gitto E. 2000. Actions of melatonin in the reduction of oxidative stress. Journal of Biomedical Science 7(6): 444–458.

Roca J, Rodríguez M J, Gil M A, Carvajal G, Garcia E M, Cuello C, Vazquez J M and Martinez E A. 2005. Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. Andrology 26(1): 15– 24.

Rodriguez C, Mayo J C, Sainz R M, Antolín I, Herrera F, Martín V and Reiter R J. 2004. Regulation of antioxidant enzymes: a significant role for melatonin. Journal of Pineal Research 36(1): 1–9.

Shahandeh E, Ghorbani M, Mokhlesabadifarahani T and Bardestani F. 2022. Melatonin and selenium supplementation in extenders improves the post-thaw quality parameters of rat sperm. Daehan Saengsik Uihak Hoeji/Clinical and Experimental Reproductive Medicine 49(2): 87–92.

Slanar O, Pelisek V and Vanecek J. 2000. Melatonin inhibits pituitary adenylyl cyclase-activating polypeptide-induced increase of cyclic AMP accumulation and [Ca2+] i in cultured cells of neonatal rat pituitary. Neurochemistry International 36(3): 213–219.

Suarez S S and Ho H C. 2003. Hyperactivated motility in sperm. Reproduction in Domestic Animals 38: 119–124.

Widyastuti R, Prastowo S, Jaswandi J, Lubis A, Setiawan R, Ridlo M R and Boediono A. 2024. Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders. Veterinary World 863–870.

Yadav H P, Kumar A, Shah N, Chauhan D S, Saxena A, Yadav S and Swain D K. 2017. Effect of cholesterol loaded cyclodextrin supplementation on tyrosine phosphorylation and apoptosis like changes in frozen-thawed Hariana bull spermatozoa. Theriogenology 96: 164–171.