Effect of supplementation of L-carnitine during in vitro maturation on embryo production in Sahiwal cows

Dr. Khushpreet Singh; Dr Narinder Singh; Dr Gurjot Kaur Mavi

doi:10.56093/ijans.v96i2.170277

Authors

Dr. Khushpreet Singh Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Dr Narinder Singh Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Dr Gurjot Kaur Mavi Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana

https://doi.org/10.56093/ijans.v96i2.170277

Keywords:

blastocyst, in vitro maturation, lipid, L-carnitine, ovum pick-up, Sahiwal

Abstract

Embryo transfer technology enables the rapid dissemination of superior genetics and in vitro produced embryos have gained more consideration than the in vivo derived embryos due to higher yield of embryos with respect to time. The primary limitation impacting in vitro derived embryos is the success of in vitro maturation as in vitro derived oocytes contain more lipid content contributing to increased oxidative stress and compromised developmental competence. Lipid modulator, L-carnitine at 1 mM and 3 mM was supplemented in the maturation media with the view to decrease lipid content and subsequent oxidative stress in the oocytes undergoing maturation. A total of 326 follicles were aspirated out of which 228 cumulus oocyte complexes (COCs) were collected at a recovery rate of 69.93%. The in vitro maturation of the COC’s supplementation with 3 mM L-Carnitine exhibited (n, %) significantly (p<0.05) lower complete expansion of cumulus cells (48, 75.00) in comparison to maturation media supplemented with 1 mM L-carnitine (58, 90.62). The cleavage rate of the COC’s matured with supplementation of 3 mM L-Carnitine displayed (Mean± Std. Error, %) considerably (p<0.05) less cleavage (7.67±0.33, 75.40) in comparison to group supplemented with 1 mM L-Carnitine (8.67 ±0.21, 83.87). The blastocyst rate of the COC’s matured with supplementation of 3 mM L-Carnitine showed (Mean± Std. Error, %) significantly (p<0.05) lower embryo production (4.17± 0.31, 40.98) in comparison to group supplemented with 1 mM L-Carnitine (5.83± 0.80, 56.45). So, the supplementation of 1 mM L-carnitine is suitable for Sahiwal oocytes during in vitro maturation for higher embryo production.

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Author Biographies

Dr. Khushpreet Singh, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana

Assistant professor

Department of Teaching Veterinary Clinical Complex
Dr Narinder Singh, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana

Principal Scientist

Directorate of Livestock Farms
Dr Gurjot Kaur Mavi, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana

Scientist

Directorate of Livestock Farms

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