Luteal Dynamics and Fertility Response to Post-Insemination hCG and Progesterone in Sub-Fertile Buffaloes

MRIGANK HONPARKHE; Jatandeep Singh Sangha; Amarjeet  Bisla; Navdeep Singh Ratta; Prahlad Singh

doi:10.56093/ijans.v96i5.171325

Authors

MRIGANK HONPARKHE
Jatandeep Singh Sangha
Amarjeet Bisla
Navdeep Singh Ratta
Prahlad Singh

https://doi.org/10.56093/ijans.v96i5.171325

Keywords:

Buffalo, Sub-fertility, Progesterone, hCG, Doppler

Abstract

Luteal insufficiency, marked by inadequate progesterone (P₄) secretion, is a key cause of early embryonic mortality in buffaloes. This study evaluated whether post-insemination administration of human chorionic gonadotropin (hCG) or P₄ could enhance luteal function and fertility in sub-fertile buffaloes. Thirty buffaloes free from subclinical endometritis and ovulatory defect were inseminated and assigned to three groups: control (saline), hCG (1500 IU on Days 4 and 11 post-AI), and P₄ (500 mg hydroxyprogesterone caproate on Days 4 and 11 post-AI). Ovarian structures and luteal blood flow were assessed using B-mode and Doppler ultrasonography, and pregnancy was diagnosed on day 55-60 post-AI. Conception rates were highest in the P₄ group (70%), followed by hCG (50%) and control (10%). Pre-ovulatory follicle size showed a positive correlation with CL area (p<0.05) and blood supply (p>0.05), but not with plasma P₄ concentration. CL area correlated significantly with blood supply (p<0.05), while plasma P₄ values on Days 4, 11, and 21 post-AI showed strong intra-day correlations. These findings confirm that P₄ and hCG supplementation, particularly P₄, improve conception in sub-fertile buffaloes by enhancing luteal growth, vascularization, and functional capacity, thereby mitigating luteal insufficiency.

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Author Biographies

MRIGANK HONPARKHE

Assistant Gynaecologist, Department of Veterinary Gynaecology and Obstetrics
Jatandeep Singh Sangha

Department of Veterinary Gynaecology and Obstetrics

College of Veterinary Science (Ludhiana)

Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Amarjeet Bisla

Department of Veterinary Gynaecology and Obstetrics

College of Veterinary Science (Ludhiana)

Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Navdeep Singh Ratta

Directorate of Livestock Farms

College of Veterinary Science (Ludhiana)

Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Prahlad Singh

Department of Teaching Veterinary Clinical Complex

College of Veterinary Science (Ludhiana)

Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana

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