Exploring the potential of Lawsonia inermis green nanoparticles to mitigate quorum sensing in multi drug resistant ESBL producing E. coli

S  CHOUDHARY; S  UPADHYAY; R  AHLAWAT; V  JAISWAL; A K  VERMA; A  MALIK; A  KUMAR

doi:10.56093/ijans.v95i9.171637

Authors

S CHOUDHARY College of Biotechnology, SVPUA&T, Modipuram, Meerut-250110, India
S UPADHYAY College of Biotechnology, SVPUA&T, Modipuram, Meerut-250110, India
R AHLAWAT SRM Institute of Science and Technology, Delhi NCR Campus, Modinagar, Ghaziabad - 201204, India
V JAISWAL College of Veterinary Sciences, SVPUA&T, Modipuram, Meerut-250110, India
A K VERMA College of Veterinary Sciences, SVPUA&T, Modipuram, Meerut-250110, India
A MALIK Dayanand College, Hisar – 125001, India
A KUMAR College of Biotechnology, SVPUA&T, Modipuram, Meerut-250110, India

https://doi.org/10.56093/ijans.v95i9.171637

Keywords:

Antibacterial, AMR, Nanoparticles, Quorum sensing

Abstract

The green synthesis of nanoparticles (NPs) has shown promising results in targeting virulence, pathogenicity and quorum sensing (QS) of bacteria and are presently being considered as a promising anti-infective drug target. Thus, this study included synthesis of green zinc nanoparticles using Lawsonia inermis (heena) leaves and their characterization by UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM). In-vitro efficacy was assessed against E. coli isolates confirmed by uidA gene primers carrying ESBL producing blaTEM, blaSHV, ctxm-1, blaCTXM-2, blaCTXM-9, oxa-48 and autoinducer luxS and pfs genes. Finally, MIC of henna nanoparticles was tested for effect on real time expression of luxS gene. UV–Vis spectra showed typical absorption peaks in around 330, 340 nm, 360 nm corresponding to ethanolic and methanolic, aqueous NPs, respectively. FT-IR analyses confirmed chemical bond formation of zinc oxide and TEM revealed the size of NPs as nearly 16nm with spherical shape. Among 42 samples, 9 were confirmed by uidA and out of these revealed only 8 isolates carried ESBL producing genes blaTEM and blaSHV, 4 blaCTXM-1 and 2 genes while one isolate had blaOXA- 48- like gene. The ABST revealed 5 out of nine isolates as MDR E. coli. The phenotypic double disc sensitivity test revealed one isolate suggestive of ESBL resistance. The 4 isolates carrying blaCTXM-1 and 2 genes also found positive for luxS and pfs autoinducer genes. The incubation of MDR ESBL producing and luxS autoinducer genes carrying isolates with Heena aqueous extract based nanoparticle at the concentration of 62.5 µg/mL down regulated the expression of luxS auto inducer genes up to 18 hrs of incubation. These findings are suggestive of the potential role of green nanoparticles to mitigate quorum sensing in MDR bacteria. However, validation with large number of MDR bacteria is still required.

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