Optimizing semen collection, dilution, and thawing protocols to enhance frozen thawed sperm quality in Bach Thao bucks

PHAN  NHAN; NGUYEN THI  CHUC

doi:10.56093/ijans.v95i11.172010

Authors

PHAN NHAN Tay Do University, 68 Tran Chien Street, Cai Rang Ward, Can Tho 900000, Vietnam
NGUYEN THI CHUC Tay Do University, 68 Tran Chien Street, Cai Rang Ward, Can Tho 900000, Vietnam

https://doi.org/10.56093/ijans.v95i11.172010

Keywords:

Artificial insemination, Bach Thao goat, Cryopreservation, dilution ratio, Semen collection interval, Sperm motility

Abstract

Artificial insemination and semen cryopreservation are key techniques for improving reproductive efficiency and conserving genetic resources in goats, but the success of these procedures depends on semen collection and processing protocols. This study aimed to optimize semen collection intervals, dilution ratios, and thawing conditions to enhance post-thaw sperm quality in Bach Thao bucks, an indigenous Vietnamese breed. Ejaculates were collected at different intervals, diluted with varying ratios of citrate-based extender, frozen, and thawed under several temperature-time combinations. Post-thaw quality of semen was evaluated through sperm motility,
morphology, and membrane integrity assessments. Semen collected at moderate intervals resulted in better sperm motility and fewer abnormalities than daily collection. A balanced dilution ratio improved cryosurvival, while excessive dilution reduced sperm quality. Thawing at moderate temperatures for short durations resulted in higher motility and membrane stability compared with lower or prolonged conditions. Integrating an appropriate collection interval with optimal dilution and controlled thawing significantly improved frozen–thawed semen quality in Bach Thao bucks. These results provide practical recommendations for breeding centers and contribute to the conservation of indigenous goat genetic resources.

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