Manuka honey in skim milk- or egg yolk-based extenders impacts stallion chilled spermatozoa characteristics, sperm-free enzymatic activity and lipid peroxidation

ISLAM M ABD EL-FATTAH; AHMED R M  ELKHAWAGAH; MOHAMED M M  KANDIEL

doi:10.56093/ijans.v96i7.172076

Authors

ISLAM M ABD EL-FATTAH Armed Forces Veterinary Hospital, Egyptian Armed Forces, Cairo, Egypt
AHMED R M ELKHAWAGAH Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, 13736, Qalioubeya, Egypt
MOHAMED M M KANDIEL Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, 13736, Qalioubeya, Egypt

https://doi.org/10.56093/ijans.v96i7.172076

Keywords:

Antioxidant, Dimitropoulos extender, Kenney extender, Manuka honey, Stallion semen

Abstract

To investigate impact of manuka honey (MH) on chilled spermatozoa characteristics, sperm-free enzymatic activity and lipid peroxidation, stallion semen samples (n=48) were diluted with Kenney (Skim milk-based, Ken) and Dimitropoulos (Egg yolk-based, Dimitri) extenders supplemented with MH at 0, 1, 2, and 4 %, and maintained at 4 °C for 72 hours. Progressive motility, livability, and morphology of sperm along with, acid phosphatase (ACP), aspartate aminotransferase (AST), acrosin, catalase (CAT), superoxide dismutase, glutathione peroxidase, and malondialdehyde (MDA) of semen were assessed at 12-hour intervals. Results showed that MH (2%) in Ken improved livability at 24-72 hrs and in Dimitri extenders enhanced motility at 72 hrs. ACP decreased with MH 2% in Ken at 0 (P= 0.007) and 12 hrs (P= 0.03), and in Dimitri at 12 (P= 0.001), 36, 48 (P= 0.001), 60 and 72 hours (P= 0.002). MH (1-4%) in both extenders markedly (P< 0.001) reduced AST and increased CAT up to 72 hrs, with 2% MH showing a profound effect. The acrosin enzyme markedly (P< 0.05) decreased in 2% MH-Ken group during the storage period. MH 2%-Ken at 0 and 12 hrs (P= 0.007 and 0.03) and Dimitri up to 72 hrs showed decreased (P< 0.001) MDA levels. MH with Ken/Dimitri extenders had a powerful effect in improving stallion chilled semen properties. MH-Ken was superior in improving semen characteristics, antioxidant defense, and potential fertilizing capacity while MH-Dimitri was good at maintaining wall integrity and shielding lipid peroxidation.

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