Manuka honey in skim milk- or egg yolk-based extenders impacts stallion chilled spermatozoa characteristics, sperm-free enzymatic activity and lipid peroxidation

Islam Abd El-Fattah; Ahmed Alkhawagah; Mohamed Kandiel

doi:10.56093/ijans.v96i7.172076

Authors

Islam Abd El-Fattah
Ahmed Alkhawagah
Mohamed Kandiel Benh University

https://doi.org/10.56093/ijans.v96i7.172076

Keywords:

Arabian stallion, Chilled semen, Dimitropoulos extender, Kenney extender, Manuka honey

Abstract

To investigate Manuka honey (MH), monofloral honey, impact on stallion chilled spermatozoa characters, sperm-free enzymatic activity and lipid peroxidation, semen samples (n=48) were diluted with Kenney (Skim milk-based, Ken) and Dimitropoulos (Egg yolk-based, Dimitri) extenders supplemented with MH at 0, 1, 2, and 4 %, and maintained at 4 °C for 72 hours. At 12-hour intervals, progressive motility, livability, and morphology, acid (ACP), aspartate aminotransferase (AST), acrosin, catalase (CAT), superoxide dismutase, glutathione peroxidase, and malondialdehyde (MDA) were assessed. Results showed that MH (2%) in Ken and Dimitri extenders improved livability 24-72 hours, and motility at 72 hours, respectively. ACP decreased with MH 2%-Ken at 0 (P= 0.007) and 12 hours (P= 0.03), and in Dimitri at 12 (P= 0.001), 36, 48 (P= 0.001), 60, and 72 (P= 0.002) hours. MH (1-4%) in both extenders markedly (P< 0.001) reduced AST and increased CAT up to 72 hours, with 2% MH showing a profound effect. The acrosin enzyme markedly (P< 0.05) decreased in the 2% MH-Ken group during storage period. MH 2%-Ken at 0 and 12 hours (P= 0.007 and 0.03) and Dimitri up to 72 hours decreased (P< 0.001) MDA levels. In conclusion, MH with Ken/Dimitri extenders has a powerful effect in improving stallion chilled semen properties. MH-Ken is superior in improving semen characteristics, antioxidant defense, and potential fertilizing capacity. MH-Dimitri is good at maintaining wall integrity and shielding lipid peroxidation.

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Author Biography

Ahmed Alkhawagah

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Egypt

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