Immune Response Study of Outer Membrane Vesicles from Salmonella enterica Spp             Associated with Poultry Enteritis

Deepak Kumar Sharma

doi:10.56093/ijans.v96i6.172893

Authors

Deepak Kumar Sharma College of veterinary and animal science, Navania Udaipur, Rajasthan university of veterinary and animal science. Bikaner

https://doi.org/10.56093/ijans.v96i6.172893

Keywords:

Humoral immune response, outer membrane vesicles, Salmonella Weltevreden, subunit vaccine,

Abstract

Salmonella enterica serovar Typhimurium var Weltevreden (S. Weltevreden) is a common foodborne pathogen in South-East Asia, raising significant public health concerns. This study investigated the efficacy of an outer-membrane vesicle (OMV)-based vaccine against S. Weltevreden in chickens. OMVs were extracted from strains isolated from poultry enteritis cases. Four groups of 10-day-old chickens (six per group) were used: three received 100 μg OMVs in 100 μl Dulbecco's Phosphate-Buffered Saline (DPBS) via intranasal (I.N.), intramuscular (I.M.), or subcutaneous (S.C.) routes, while the fourth group received DPBS only as a control. A booster dose was administered on day 14. Blood samples were collected on days 0, 7, 14, 21, and 28 post-vaccination to assess IgG levels using indirect ELISA. The S.C. and I.N. groups showed a significant IgG increase by day 7 post-primary immunization, followed by a slight decline until day 14. After the booster, IgG levels rose significantly through day 28 (p ≤ 0.01). The I.M. group exhibited a non-significant IgG rise initially, with a similar decline by day 14, but showed a significant increase post-booster. These results suggest that OMV-based vaccines, particularly via S.C. and I.N. routes, effectively stimulate humoral immunity in chickens. While promising, further clinical and immunological evaluations are necessary to confirm their protective efficacy. Given the zoonotic potential of S. Weltevreden, OMV vaccines may offer a viable strategy for controlling infections in poultry and reducing transmission risks to humans.

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Author Biography

Deepak Kumar Sharma, College of veterinary and animal science, Navania Udaipur, Rajasthan university of veterinary and animal science. Bikaner

Assistant professor, department of veterinary microbiology

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