Impact of season, age and breed on semen microbial load andits association with semen quality in prominent Indian goat breeds

ABHISHEK KUMAR  VERMA; BHOOPENDRA  SINGH; SUSHANT  SRIVASTAVA; KABIR  ALAM; SANJEEV KUMAR  VERMA; RAJESH KUMAR  VERMA; SAURABH; RABINDRA  KUMAR; RAJESH  KUMAR; ANKIT  JAISWAL

doi:10.56093/ijans.v96i1.172978

Authors

ABHISHEK KUMAR VERMA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
BHOOPENDRA SINGH Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
SUSHANT SRIVASTAVA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
KABIR ALAM Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
SANJEEV KUMAR VERMA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
RAJESH KUMAR VERMA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
SAURABH Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
RABINDRA KUMAR Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
RAJESH KUMAR Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India
ANKIT JAISWAL Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya Uttar Pradesh 224 229 India

https://doi.org/10.56093/ijans.v96i1.172978

Keywords:

Bacteriospermia, Barbari, Buck semen, Jamnapari, Microbial load, Sirohi

Abstract

The aim of this study was to estimate the occurrence of bacteriospermia, determine the bacterial load in fresh semen from 2–6-year-old bucks, and assess the association between bacterial load and sperm quality traits in various Indian goat breeds. For the experiment, one hundred forty-four (144) fresh semen ejaculates were obtained from six bucks of various breeds (two bucks from each breed: Jamnapari, Sirohi, and Barbari) using a sterile artificial vagina on a weekly basis, adhering to a standardized collection technique during three distinct seasons (summer, rainy, and winter). After initial evaluation, samples were subjected to bacteriological examination. The total viable bacterial count of fresh semen was measured using the standard plate count method. The results indicated that the bacterial load was 70%, 56.66%, and 63.33% in Sirohi, Jamnapari, and Barbari bucks, respectively. Sirohi bucks exhibited higher bacterial counts (458.36 ± 9.35 CFU/mL) compared to both Barbari (419.28 ± 10.23 CFU/mL) and Jamnapari (413.82 ± 9.77 CFU/mL). An age-wise analysis revealed that older bucks (≥5 years: 456.73±12.56 CFU/mL) had significantly greater microbial loads than younger groups (2.5–3.5 years: 406.90±4.33 CFU/mL; 3.5–5 years: 423.40±9.53 CFU/mL). Microbial loads in rainy season were significant (p<0.001) higher than those observed in winter and summer. Moreover, Pearson correlation analysis revealed that a higher microbial load in buck semen was negatively associated with sperm motility, viability, and plasma membrane integrity.

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