Effect of melatonin administration on hormonal profile and oxidative stressstatus in pubertal Jaffarabadi buffalo heifers during summer season

UB  RAMOLIYA; HP  VIJYETA; R J  PADODARA; JV  VADALIA; KB  VALA; HA  ROKAD

doi:10.56093/ijans.v96i4.173900

Authors

UB RAMOLIYA Kamdhenu University, Junagadh 362 001, Gujarat, India
HP VIJYETA Kamdhenu University, Junagadh 362 001, Gujarat, India
R J PADODARA Kamdhenu University, Junagadh 362 001, Gujarat, India
JV VADALIA Kamdhenu University, Junagadh 362 001, Gujarat, India
KB VALA Kamdhenu University, Junagadh 362 001, Gujarat, India
HA ROKAD Kamdhenu University, Junagadh 362 001, Gujarat, India

https://doi.org/10.56093/ijans.v96i4.173900

Keywords:

Buffalo heifers, Hormonal profile, Lipid peroxidation, Melatonin, Progesterone, TAC

Abstract

Reproductive inefficiency during the summer season is a major challenge in buffaloes due to heat stress–induced anestrus and hormonal imbalance. The present study aimed to evaluate the effect of exogenous melatonin administration on plasma melatonin and progesterone concentrations, as well as oxidative stress markers in pubertal Jaffarabadi buffalo heifers during summer. A total of 24 buffalo heifers were divided into four equal groups: T0 (control, Corn oil), T1 (Corn oil + Ovsynch), T2 (Corn oil + Melatonin), and T3 (Corn oil + Melatonin + Ovsynch). Blood samples were collected on days 0, 4, 10 and 28 post-treatments for estimation of plasma melatonin, progesterone, lipid peroxidation (LPO) and total antioxidant capacity (TAC). The results revealed a significant rise (p<0.05) in plasma melatonin levels on day 4 in T2 (409 ± 26.10 pg/ml) and T3 (451 ± 24.00 pg/ml) compared to control groups, followed by a gradual decline by day 28. Progesterone level increased non-significantly on day 10 in melatonin-treated groups and was highest post-AI in T3, indicating stronger luteal support. Melatonin supplementation significantly (p<0.05) reduced LPO and elevated TAC on days 4 and 10 in T2 and T3, indicating mitigation of heat-induced oxidative stress. The study concluded that melatonin improved endocrine function and antioxidant status, making it beneficial for managing summer anestrus in buffalo heifers.

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References

Abulaiti A, Nawaz M, Naseer Z, Ahmed Z, Liu W, Abdelrahman M and Wang S. 2023. Administration of melatonin prior to modified synchronization protocol improves the productive and reproductive efficiency of Chinese crossbred buffaloes in low breeding season. Frontiers in Veterinary Science 10: 1118604. DOI: https://doi.org/10.3389/fvets.2023.1118604

Bashandy S A, Ebaid H, Al-Tamimi J, Ahmed-Farid O A-H, Omara E A and Alhazza I M. 2021. Melatonin alleviated potassium dichromate-induced oxidative stress and reprotoxicity in male rats. BioMed Research International 1: 3565360. DOI: https://doi.org/10.1155/2021/3565360

Benot S, Molinero P, Soutto M, Goberna R and Guerrero J M. 1998. Circadian variations in the rat serum total antioxidant status: Correlation with melatonin levels. Journal of Pineal Research 25(1): 1–4. DOI: https://doi.org/10.1111/j.1600-079X.1998.tb00378.x

Chemineau P, Malpaux B, Delgadillo J A, Guérin Y, Ravault J P, Thimonier J and Pelletier J. 1992. Control of sheep and goat reproduction: Use of light and melatonin. Animal Reproduction Science 30(1-3): 157-184. DOI: https://doi.org/10.1016/0378-4320(92)90010-B

Dash S, Chakravarty A K, Sah V, Jamuna V, Behera R, Kashyap N, and Deshmukh B. 2015. Influence of temperature and humidity on pregnancy rate of Murrah buffaloes under subtropical climate. Asian-Australasian Journal of Animal Sciences 28(7): 943. DOI: https://doi.org/10.5713/ajas.14.0825

Deb G K, Nahar T N, Duran P G and Presicce G A 2016. Safe and sustainable traditional production: The water buffalo in Asia. Frontiers in Environmental Science 4: 38. DOI: https://doi.org/10.3389/fenvs.2016.00038

Georgieva N V. 2005. Oxidative stress as a factor of disrupted ecological oxidative balance in biological systems - A review. Bulgarian Journal of Veterinary Medicine 8(1): 1–11.

Ghuman S S, Honparkhe M, and Singh B. 2023. Assessment of plasma melatonin profile in summer anestrous buffaloes exhibiting differential fertility following melatonin implants treatment. Buffalo Bulletin 42(3): 407–415. DOI: https://doi.org/10.56825/bufbu.2023.4233571

Kang J T, Koo O J, Kwon D K, Park H J, Jang G, Kang S K and Lee B C. 2009. Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells. Journal of Pineal Research 46(1): 22-28. DOI: https://doi.org/10.1111/j.1600-079X.2008.00602.x

Kumar A, Mehrotra S, Singh G, Maurya V P, Narayanan K, Mahla A S, Chaudhary R K, Singh M, Soni Y K, Kumawat B L, Dabas S K and Srivastava N. 2016. Supplementation of slow‐release melatonin improves recovery of ovarian cyclicity and conception in summer DOI: https://doi.org/10.1111/rda.12639

anoestrous buffaloes (Bubalus bubalis). Reproduction in Domestic Animals 51(1): 10–17.

Kumar A, Mehrotra S, Singh G, Narayanan K, Das G K, Soni Y K and Verma M R. 2015. Sustained delivery of exogenous melatonin nfluences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (Bubalus bubalis). Theriogenology 83(9): 1402–1407. DOI: https://doi.org/10.1016/j.theriogenology.2014.12.023

Manjunatha B M, Devaraj M, Gupta P S P, Ravindra J P and Nandi S. 2009. Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development. Reproduction in Domestic Animals 44(1): 12–16. DOI: https://doi.org/10.1111/j.1439-0531.2007.00982.x

Michael J D, Ghuman S S, Neglia G, Della Valle G, Baruselli P S, Zicarelli L, Visintin J A, Sarkar M and Campanile G. 2020. Exogenous and endogenous factors in seasonality of reproduction in buffalo: A review. Theriogenology 150: 186–192. DOI: https://doi.org/10.1016/j.theriogenology.2020.01.044

Perumal P, De A K, Bhattacharya D, Alyethodi R R and Kundu A. 2021. Effect of exogenous melatonin on endocrinological profiles, biochemical and antioxidant and oxidative stress profiles in postpartum anestrus Andaman local buffaloes in tropical island ecosystem. Indian Journal of Animal Sciences 91(3): 206–213. DOI: https://doi.org/10.56093/ijans.v91i3.114145

Phogat J B, Pandey A K, Balhara A K, Ghuman S S and Gunwant P 2018. Effects of melatonin supplementation prior to Ovsynch protocol on ovarian activity and conception rates in anestrous Murrah buffalo heifers during out of breeding season. Reproductive Biology 18(2): 161–168. DOI: https://doi.org/10.1016/j.repbio.2018.03.001

Reiter R J, Tan D X, Rosales-Corral S, Galano A, Zhou X J and Xu B. 2018. Mitochondria: central organelles for melatonin’s antioxidant and anti-aging actions. Molecules 23(2): 509. DOI: https://doi.org/10.3390/molecules23020509

Takasaki A, Nakamura Y, Tamura H, Shimamura K and Morioka H. 2003. Melatonin as a new drug for improving oocyte quality. Reproductive Medicine and Biology 2: 139–144. DOI: https://doi.org/10.1111/j.1447-0578.2003.00035.x

Zarezadeh M, Barzegari M, Aghapour B, Adeli S, Khademi F, Musazadeh V and Ostadrahimi A. 2022. Melatonin effectiveness in amelioration of oxidative stress and strengthening of antioxidant defense system: Findings from a systematic review and dose–response meta-analysis of controlled clinical trials. Clinical Nutrition: The European Society for Clinical Nutrition and Metabolism 48: 109–120. DOI: https://doi.org/10.1016/j.clnesp.2022.01.038