In vitro anthelmintic efficacy of aqueous extracts from Coriandrum sativum seeds and Swertia chirata plant against Haemonchus contortus in Barbari goats

RAMAKANT; Arbind Singh; Prem Sagar Maurya; TARUN KUMAR  SARKAR; VIKAS  JAISWAL; DEVESH KUMAR  YADAV; AMIT KUMAR  VERMA

doi:10.56093/ijans.v96i7.175813

Authors

RAMAKANT Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology Kumarganj-224 229 Ayodhya, Uttar Pradesh, India
Arbind Singh Department of Livestock Farm Complex, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India
Prem Sagar Maurya Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India
TARUN KUMAR SARKAR Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India
VIKAS JAISWAL Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India
DEVESH KUMAR YADAV Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India
AMIT KUMAR VERMA Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut-250 110, India

https://doi.org/10.56093/ijans.v96i7.175813

Keywords:

Anthelmintic activity, Barbari goat, Coriandrum sativum, Haemonchus contortus, In vitro assay, Swertia chirata

Abstract

The present study explored the laboratory-based anti-parasitic potential of water extracts of coriander (Coriandrum sativum) seeds and entire Swertia chirata plant against Haemonchus contortus parasite, collected directly from Barbari goats. The faecal samples were cultured to harvest infectious third-stage (L3) larvae. Subsequently, PCR testing on the ITS-2 ribosomal DNA region was utilized to verify the parasite as H. contortus. To evaluate the deworming potential of the plant extracts, both egg hatch assays (EHA) and larval paralysis assays (LPA) were performed at four different dosage levels: 12.5, 25, 50, and 100 mg/ml. Phosphate-buffered saline (PBS) was used as a negative baseline, while Closantel (0.5 mg/ml) served as the positive control. Findings revealed that the coriander seed extract showed dose dependent effectiveness, successfully preventing 27.49% to 86.94% of the eggs from hatching and paralyzing 35.07% to 89.93% of the larvae. However, aqueous extract of S. chirata proved to be considerably more effective. It restricted 43.64 and 93.12%, egg hatching and stopped larval movement by 47.40% to 94.10%. As expected, the closantel control achieved total elimination in both tests. Ultimately, these experiments demonstrated that both botanical extracts successfully cleared H. contortus in a lab setting, with S. chirata standing out as the highly superior candidate.

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