Adjunctive effect of ascorbic acid on tetracycline treatment inbroiler chickens challenged with tetracycline-resistant Escherichia coli

RAMASAMY  THANGAMALAI; PAVITHRA  M; RAMESH S; ELAMARAN A; JAIKANTH C M; M AKSHAY DURGA  PRASAD; SAMUEL MASILAMONI  RONALD

doi:10.56093/ijans.v96i2.175877

Authors

RAMASAMY THANGAMALAI Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
PAVITHRA M Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
RAMESH S Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
ELAMARAN A Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
JAIKANTH C M Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
M AKSHAY DURGA PRASAD Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India
SAMUEL MASILAMONI RONALD Tamil Nadu Veterinary and Animal Sciences University, Chennai Tamil Nadu 636 112 India

https://doi.org/10.56093/ijans.v96i2.175877

Keywords:

Ascorbic acid pretreatment, Broiler chicken, Tetracycline Resistance, tet A gene, tet B gene

Abstract

The emergence of multidrug-resistant organisms due to the indiscriminate use of antibacterials has become a critical health concern for humans and animals. This study aimed to explore the potential of ascorbic acid to enhance the in vitro and in vivo effectiveness of tetracycline against Avian Pathogenic Escherichia coli (APEC) infections resistant to tetracycline. Isolates of E. coli from broiler chickens affected by colibacillosis were screened for the presence of tetracycline-resistant genes. The in vitro antibacterial properties of ascorbic acid were evaluated. In the in vivo experiment, the birds were pre-treated with ascorbic acid beginning on day three, and on day seventeen of trial, they were challenged with tetracycline-resistant APEC. Clinical symptoms of infection were observed two days
post-challenge and then treated with tetracycline for 5 days continuously. After five days of tetracycline treatment, blood samples and organ tissues were collected for haematological, biochemical and histopathological analyses. The study revealed that 60% of prevalence rate for APEC isolates showing the highest resistance with tetracycline. The combination of ascorbic acid did not markedly enhance the antibacterial efficacy of tetracycline in vitro, its administration in vivo has notably diminished disease severity. Although no marked in vitro synergistic effect was observed, ascorbic acid supplementation was associated with reduced disease severity in vivo, suggesting a potential supportive role during antibacterial therapy that warrants further investigation.

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References

Abdelraheem W M, Refaie M M, Yousef R K M, Abd El Fatah A S, Mousa Y M and Rashwan R. 2022. Assessment of antibacterial and anti-biofilm effects of vitamin C against Pseudomonas aeruginosa clinical isolates. Frontiers in Microbiology 13: 847449. DOI: https://doi.org/10.3389/fmicb.2022.847449

Amin U, Kamil S A, Wani B M, Qureshi S, Shah S A, Dar T A and Mir M S. 2020. Haematological and biochemical alterations of broiler chicken. affected naturally with colibacillosis. International Journal of Current Microbiology and Applied Sciences 9: 1906–1913. DOI: https://doi.org/10.20546/ijcmas.2020.906.236

Awaad M H H, El-Moustafa K M, Zoulfakar S A, Elhalawany M S, Mohammed F F, El-Reay R M and Morsy E A. 2021. The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens. Journal of Applied Poultry Research 30: 100155. DOI: https://doi.org/10.1016/j.japr.2021.100155

Bhardwaj K, Suchitra S M, Baliga S, Unnikrishnan B, Shantharam B and Shetty V K. 2021. Research Note: Characterization of antibiotic-resistant phenotypes and linked genes of Escherichia coli and Klebsiella pneumoniae from healthy broiler chickens. Karnataka, India. Poultry Science 100: 101094. DOI: https://doi.org/10.1016/j.psj.2021.101094

Bonassa K P, Miragliotta M Y, Simas R C, Eberlin M N, Anadón A, Moreno R A and Reyes F G. 2021. Pharmacokinetics, Pharmacodynamic efficacy prediction indexes and Monte Carlo Simulations of Enrofloxacin hydrochloride against bacterial strains that induce common clinical diseases in broiler chickens. Frontiers in Veterinary Science 7: 606872. DOI: https://doi.org/10.3389/fvets.2020.606872

El-Shafei R A and Saleh R M. 2016. Pharmacological effects of vitamin C & E on diclofenac sodium intoxicated rats. Biomedicine and Pharmacotherapy 84: 314–322. DOI: https://doi.org/10.1016/j.biopha.2016.09.005

Fang H and Hedin G. 2003. Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. Journal of Clinical Microbiology 41(7): 2894–2899. DOI: https://doi.org/10.1128/JCM.41.7.2894-2899.2003

Goudarztalejerdi A, Mohammadzadeh A, Niazi K and Mohammad Mirzaei M. 2022. High prevalence of multidrug resistance and biofilm-formation ability among avian Escherichia coli isolated from broilers in Iran. Microbial Drug Resistance 28: 244–254. DOI: https://doi.org/10.1089/mdr.2021.0091

Grakh K, Mittal D, Prakash A and Jindal N. 2022. Characterization and antimicrobial susceptibility of biofilm-producing Avian Pathogenic Escherichia coli from broiler chickens and their environment in India. Veterinary Research Communications 46: 537–548. DOI: https://doi.org/10.1007/s11259-021-09881-5

Hashem M A, Neamat-Allah A N, Hammza H E and Abou-Elnaga H M. 2019. Impact of dietary supplementation with Echinacea purpurea on growth performance, immunological, biochemical, and pathological findings in broiler chickens infected by pathogenic E. coli. Tropical Animal Health and Production 52: 1599–1607. DOI: https://doi.org/10.1007/s11250-019-02162-z

Hassuna N A, Rabie E M, Mahd W K M, Refaie M M, Yousef R K M and Abdelraheem W M. 2023. Antibacterial effect of vitamin C against uropathogenic E. coli in vitro and in vivo. BMC Microbiology 23(1): 112. DOI: https://doi.org/10.1186/s12866-023-02856-3

Jaensch S and Clark P. 2004. Haematological characteristics of response to inflammation or traumatic injury in two species of black cockatoos: Calyptorhynchus magnificus and C. funereus. Comparative Clinical Pathology 13: 9–13. DOI: https://doi.org/10.1007/s00580-004-0510-x

Khaldoun Oularbi H, Richeval C, Lebaili N, Zerrouki-Daoudi N, Baha M, Djennas N and Allorge D. 2017. Ameliorative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats. Human and Experimental Toxicology 36(7): 709–717. DOI: https://doi.org/10.1177/0960327116661022

Mousavi S, Escher U, Thunhorst E, Kittler S, Kehrenberg C, Bereswill S and Heimesaat M M. 2020. Ascorbic acid alleviates acute enteroclolitis in Camphylobacter jejuni infected mice. Scientific Reports 10: 2921. DOI: https://doi.org/10.1038/s41598-020-59890-8

Rahal A and Kumar A. 2021. Strategies to combat antimicrobial resistance in Indian scenario. The Indian Journal of Animal Sciences 91: 79–90. DOI: https://doi.org/10.56093/ijans.v91i2.113812

Ramasamy T, Keerthana S, Srinivasan M R, Chandrasekar D, Porteen K, Borthakur A, Elamaran A and Sriram P. 2021. Molecular characterization of antibiotic resistance gene pattern of Staphylococcus aureus and Escherichia coli in mastitis affected dairy cows. Indian Journal of Animal Research 55: 463–468. DOI: https://doi.org/10.18805/IJAR.B-3972

Rosa G, Alba D F, Silva A D, Gris A, Mendes R E, Mostardeiro V B, Lopes T F, Schetinger M R C, Stefani L M, Lopes M T and Boiago M M. 2020. Impact of Escherichia coli infection in broiler breeder chicks: The effect of oxidative stress on weight gain. Microbial Pathogenesis 139: 103861. DOI: https://doi.org/10.1016/j.micpath.2019.103861

Saha O, Hoque M N, Islam O K, Rahaman M, Sultana M and Hossain M A. 2020. Multidrug-resistant avian pathogenic Escherichia coli strains and association of their virulence genes in Bangladesh. Microorganisms 8: 1135. DOI: https://doi.org/10.3390/microorganisms8081135

Sarker M S, Mannan M S, Ali M Y, Bayzid M, Ahad A and Bupasha Z B. 2019. Antibiotic resistance of Escherichia coli isolated from broilers sold at live bird markets in Chattogram, Bangladesh. Journal of Advanced Veterinary and Animal Research 6: 272–277. DOI: https://doi.org/10.5455/javar.2019.f344

Shivaprasad D P, Taneja N K, Lakra A and Sachdev D. 2021. In vitro and in situ abrogation of biofilm formation in E. coli by Ascorbic acid through ROS generation, disruption of quorum sensing and exopolysaccharide production. Food Chemistry 341: 128171. DOI: https://doi.org/10.1016/j.foodchem.2020.128171

Sriraam K S, Ramasamy T, Porteen K, Elamaran A, Pavithra M, Jagadeesh T and Ramesh S. 2022. Effect of atorvastatin and vitamin D against multi-drug resistant Staphylococcus spp and Escherichia coli isolated from bovine mastitis cases. Indian Journal of Animal Research DOI: 10.18805/IJAR.B-4969. DOI: https://doi.org/10.18805/IJAR.B-4969

Surjagade S, Joshi B P, Mathakiya R A, Kapadiya B, Bansod A P, Jadhao A D, Prajapati R, Ghodasara D J, Dave C J and Jani P. 2020. Mortality pattern and pathological study of E. coli infection in broiler chickens. Journal of Entomology and Zoology Studies 8: 867–872.

Topi D and Spahiu J. 2020. Presence of veterinary antibiotics in livestock manure in two Southeastern Europe countries, Albania and Kosovo. Environmental Science and Pollution Research 27: 44552–44560. DOI: https://doi.org/10.1007/s11356-020-10341-x