Effect of dietary supplementation of phytogenic plant leaves on expression ofinsulin-like growth factor-1 (IGF-1) in the Japanese quail (Coturnix japonica)

YOGESH  PALYAL; ANSHU  RAHAL; AMAN  KAMBOJ; SHIVANGI  MAURYA; SHALINI  PANT

doi:10.56093/ijans.v96i4.176402

Authors

YOGESH PALYAL College of Veterinary and Animal Sciences, G.B Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, 263145 India
ANSHU RAHAL College of Veterinary and Animal Sciences, G.B Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, 263145 India
AMAN KAMBOJ College of Veterinary and Animal Sciences, G.B Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, 263145 India
SHIVANGI MAURYA College of Veterinary and Animal Sciences, G.B Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, 263145 India
SHALINI PANT College of Veterinary and Animal Sciences, G.B Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, 263145 India

https://doi.org/10.56093/ijans.v96i4.176402

Keywords:

Gene expression, Insulin like growth factor-1, Japanese quail, Phytogenic supplements

Abstract

Nutrigenomics is an important and emerging field that examines the interactions between feed and genes that affect health and production. The present study aligned with this direction; the expression of insulin-like growth factor-1 (IGF-1) in the muscle of Japanese quail (Coturnix japonica) was evaluated following dietary supplementation with phytogenic feed additives. The experimental trial involved 120-day-old Japanese quail chicks reared for 42 days. Birds were randomly allocated into four treatment groups of 30 birds each: T1 (control), fed a basal diet formulated according to ICAR (2013) recommendations; T2, fed basal diet supplemented with 1% Shisham (Dalbergia sissoo) leaf powder; T3, fed basal diet supplemented with 1% poplar (Populus deltoides) leaf powder; and T4, fed basal diet supplemented with 2% leaf powder comprising 1% Shisham and 1% poplar. At 42 days of age, birds were slaughtered, and muscle tissue samples were collected for IGF-1 gene expression analysis. Total RNA was isolated, and gene expression was quantified using one-step quantitative real-time Polymerase chain reaction (qRT-PCR). IGF-1 expression was found higher in all phytogenically supplemented groups compared to the control, with the highest fold increase observed in the T4 group. These findings suggested that phytogenic leaf supplementation positively influenced muscle protein development, with a synergistic effect observed when multiple phytogenic sources are combined in the diet showing the higher fold increase in the expression level of IGF-1.

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