Comparative Efficacy of Recombinant Human Follicle Stimulating Hormone and Porcine Follicle Stimulating Hormone on Follicular Response, Oocyte recovery and in vitro embryo production in Donor Cows (Bos indicus)

Mahesh V Bhalke; Shyam G Deshmukh; Chaitanya H Pawshe; Maheshkumar V Ingawale; Dilip S Raghuwanshi; Manoj S Patil; Pravin S Bankar; Kalpak B Kayande

doi:10.56093/ijans.v96i2.176491

Authors

Dr. Mahesh V Bhalke College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University
Dr S.G.Deshmukh College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University
Dr. Chaitanya H Pawshe College of Veterinary and Animal Sciences, Akola Maharashtra Animal and Fishery Sciences University
Dr M V Ingawale College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University
Dr D S Raghuwanshi Nagpur Veterinary College Nagpur affiliated to Maharashtra Animal and Fishery Sciences University
Dr M S Patil Nagpur Veterinary College Nagpur affiliated to Maharashtra Animal and Fishery Sciences University
Dr P S Bankar College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University
Dr K B Kayande College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University

https://doi.org/10.56093/ijans.v96i2.176491

Keywords:

Blastocyst rate, Follicular response, In vitro embryo production, Oocyte recovery, Recombinant FSH, Superstimulation

Abstract

The present study evaluated the comparative efficacy of long-acting recombinant human follicle stimulating hormone (rhFSH) and pituitary-derived FSH (pFSH) on follicular response, oocyte recovery and in vitro embryo production in donor cows. Eight high-yielding multiparous cows were subjected to two ovarian superstimulation protocols in a crossover design. In the first trial, donors received 200 mg pFSH in tapering doses, whereas in the second trial a single dose of long-acting rhFSH (300 IU) was administered. Transvaginal ovum pick-up (OPU) was performed on Day 8, and follicular count, oocyte recovery rate, oocyte quality, cleavage rate and blastocyst rate were recorded. The mean total follicle count did not differ significantly between pFSH (27±1.83) and rhFSH (25±2.15) stimulated groups. The oocyte recovery rate was significantly higher in the rhFSH group (77%; 154/200) compared to the pFSH group (68.05%; 147/216). The mean total oocyte yield per OPU was comparable between rhFSH (19.25±2.03) and pFSH (18.37±1.53) treatments. Cleavage rate (66.23% vs 72.1%) and blastocyst rate (55.84% vs 62.58%) did not differ significantly between rhFSH and pFSH groups, respectively. It was concluded that long-acting rhFSH is comparable to conventional pFSH in terms of follicular response, oocyte quality and embryo production, while providing significantly improved oocyte recovery efficiency.

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Author Biography

Dr S.G.Deshmukh, College of Veterinary and Animal Sciences, Akola affiliated to Maharashtra Animal and Fishery Sciences University

Dr. S. G. Deshmukh, Professor, Department of Animal Reproduction College of Veterinary and Animal Sciences, Krishi Nagar Murtijapur road, Akola (M.S.) 444 104

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