Identification of HSPA1A SNPs and heat stress biomarkers using infrared thermography and gene expression analysis in Bos indicus

P B  GHORPADE; A B  PANDE; ROHIT  VALHE; V N  KHANDAIT; P V  JADHAV; G M  GADEGAONKAR; M J  SANAP; S A  DHENGE; S S  RAMTEKE; A D  PATIL; V B  DONGRE; G S  SONAWANE; L S  KOKATE; M M  VAIDYA; POONAM  SHARMA; S R  BIRADAR; V V  KARHALE; S J  KOMATWAR; G R  CHANNA

doi:10.56093/ijans.v96i7.177287

Authors

P B GHORPADE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
A B PANDE Group Vice President, BAIF Development Research Foundation, Pune, Maharashtra, India
ROHIT VALHE Rajiv Gandhi Science and Technology Commission (RGSTC), Mumbai, India
V N KHANDAIT Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
P V JADHAV Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
G M GADEGAONKAR Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
M J SANAP Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
S A DHENGE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
S S RAMTEKE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
A D PATIL Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
V B DONGRE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
G S SONAWANE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
L S KOKATE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
M M VAIDYA Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
POONAM SHARMA Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
S R BIRADAR Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
V V KARHALE Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
S J KOMATWAR Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India
G R CHANNA Maharashtra Animal and Fishery Sciences University, Nagpur 440 001 Maharashtra India

https://doi.org/10.56093/ijans.v96i7.177287

Keywords:

Deoni cattle, HSPA1A gene, Infrared thermal imaging, Rectal temperature

Abstract

A study was conducted to evaluate seasonal thermal adaptability in 1,044 Deoni cattle representing Wanera (n = 356), Balankya (n = 560), and Shewara (n = 128) strains under semi-arid conditions of western India using infrared thermography, physiological measurements, and HSPA1A gene expression analysis. Body surface temperature was recorded from multiple anatomical regions during summer and winter, and the relationship with physiological variables and molecular markers was evaluated using a linear mixed-effects model with animal as a random effect. Surface temperature differed significantly at anatomical regions (P < 0.001) and varied with season and physiological status. Among the evaluated regions, the hump maintained a relatively stable mean surface temperature (approximately 34°C) across seasons and showed significant positive correlations with thorax, flank, and hip temperatures (r = 0.71–0.89, P < 0.001). The temperature–humidity index (THI) was moderately associated with hump temperature at morning but only weakly associated with the evening observations. No significant differences were observed in rectal temperature or respiration rate between the AA and BB genotypes (P > 0.05). Relative HSPA1A expression, quantified using the 2^−ΔΔCt method after normalization to GAPDH, was found to be inversely associated with body surface temperature during morning observations (r = −0.71, P < 0.001) but showed no significant association during evening measurements (P > 0.05). These findings suggested that infrared thermography, when combined with physiological measurements and HSPA1A expression analysis, can provide complementary information for assessing thermal status in Deoni cattle.

Downloads

Download data is not yet available.

References

1. Basiricò L, Morera P, Primi V, Lacetera N and Nardone A. 2011. Cellular thermotolerance is related to HSPA1A expression in bovine lymphocytes. Journal of Animal Science 89: 378–385.

2. Collier R J, Baumgard L H, Zimbelman R B and Xiao Y. 2019. Heat stress: Physiology of acclimation and adaptation. Animal Frontiers 9(1): 12–19. DOI: https://doi.org/10.1093/af/vfy031

3. Collier R J, Dahl G E and VanBaale M J. 2008. Major advances associated with environmental effects on dairy cattle. Journal of Dairy Science 91: 445–454.

4. Dangi S S, Gupta M, Maurya D, Yadav V P, Panda R P, Singh G, Mohan N H, Bhure S K, Das B C, Bag S and Sarkar M. 2012. Expression dynamics of HSP genes during thermal stress in livestock. International Journal of Biometeorology 56: 633–640.

5. Deb R, Sajjanar B, Singh U, Kumar S, Brahmane M P, Singh R, Sengr G and Sharma A. 2013. Genetic polymorphism and expression profiling of HSPA1A gene in relation to heat stress tolerance in Zebu and crossbred cattle. Gene 532 (2): 230–236. DOI: https://doi.org/10.1016/j.gene.2013.09.037

6. Gaughan J B, Mader T L, Holt S M and Lisle A. 2010. A new heat load index for feedlot cattle. International Journal of Biometeorology 54: 121–130.

7. Gupta S C, Sharma A and Mishra B P. 2013. Genetic polymorphism of HSPA1A gene and its association with thermo-tolerance traits in cattle. Indian Journal of Animal Sciences 83: 102–05.

8. Kamboh A A, Hyder S I, Khan M. A. 2018. Heat stress in poultry production: Mitigation strategies and heat shock protein responses. Poultry Science 97: 1–14.

9. Kiang J G and Tsokos G C. 1998. Heat shock protein 70 kDa: Molecular biology, biochemistry and physiology. Pharmacology and Therapeutics 80: 183–201. DOI: https://doi.org/10.1016/S0163-7258(98)00028-X

10. Kregel K C. 2002. Heat shock proteins: Modifying factors in physiological stress responses and acquired thermotolerance. Journal of Applied Physiology 92: 2177–2186 DOI: https://doi.org/10.1152/japplphysiol.01267.2001

11. Lindquist S and Craig E A. 1988. The heat-shock proteins. Annual Review of Genetics 22: 631–677. DOI: https://doi.org/10.1146/annurev.ge.22.120188.003215

12. Mader T L, Davis M S and Brown-Brandl T. 2006. Environmental factors influeucing heat stress in feedlot cattle. Journal of Animal Science 84: 712–719. DOI: https://doi.org/10.2527/2006.843712x

13. Mayer M P and Bukau B. 2005. Hsp70 chaperones: Cellular functions and molecular mechanism. Cellular and Molecular Life Sciences 62: 670–684. DOI: https://doi.org/10.1007/s00018-004-4464-6

14. McCafferty D J. 2007. The value of infrared thermography for research on mammals and birds. Journal of Thermal Biology 32: 247–268.

15. McManus C, Prescott E, Paludo G R, Bianchini E, Louvandini H and Mariante A S. 2009. Heat stress effects on cattle: A review. Tropical Animal Health and Production 41: 133–147.

16. Montanholi Y R, Odongo N E, Swanson K C, Schenkel F S, McBride B W, Miller S P and Montanholi C. 2008. Application of infrared thermography to identify differences in metabolic status of cattle. Journal of Animal Science 86: 2670–2678.

17. Ramesha K P, Singh V K and Verma A. 2016. Polymorphism in heat shock protein 70 gene and its association with thermotolerance in Indian dairy cattle. Tropical Animal Health and Production 48(3): 451–458.

18. Ring E F J and Ammer K. 2012. Infrared thermal imaging in medicine. Physiological Measurement 33(3): 33–46. DOI: https://doi.org/10.1088/0967-3334/33/3/R33

19. Schaefer A L, Cook N J, Bench C, Chabot J B, Colyn J, Liu T, Okine E K, Stewart M and Webster J R. 2012. The non- invasive and automated detection of bovine respiratory disease using infrared thermography. Research in Veterinary Science 93: 928–935. DOI: https://doi.org/10.1016/j.rvsc.2011.09.021

20. Sodhi M, Mukesh M, Kishore A, Mishra B P, Kataria R S and Joshi B K. 2013. Novel polymorphisms in UTR and coding region of inducible heat shock protein 70.1 gene in tropically adapted Indian zebu cattle (Bos indicus) and riverine buffalo (Babulas bubalis). Gene 572: 606–615. DOI: https://doi.org/10.1016/j.gene.2013.05.078

21. Stewart M, Stafford K J, Dowling S K, Schaefer A L and Webster J R. 2008. Eye temperature and heart rate variability in calves using infrared thermography. Physiology and Behavior 93: 789–797. DOI: https://doi.org/10.1016/j.physbeh.2007.11.044

22. West J W. 2003. Effects of heat stress on production in dairy cattle. Journal of Dairy Science 86: 2131–2144. DOI: https://doi.org/10.3168/jds.S0022-0302(03)73803-X