Breed- and lactation stage-specific expression of beta-lactoglobulin (LGB) and alpha-lactalbumin (LAA) genes in milk-derived primary mammary epithelial cells of Surti and Mehsani buffaloes (Bubalus bubalis)

Kuldeep Kumar Tyagi; BALKRISHNA PRATAPCHAND BRAHMKSHTRI; UMED VITTHALBHAI RAMANI; GAURAV MULVANT PANDYA

doi:10.56093/ijans.v96i7.177311

Authors

Kuldeep Kumar Tyagi Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut- 250110
BALKRISHNA PRATAPCHAND BRAHMKSHTRI Professor & Ex Dean, Veterinary College Himmatnagar, Kamdhenu University, Gujarat
UMED VITTHALBHAI RAMANI Associate Professor, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat
GAURAV MULVANT PANDYA Associate Professor, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat

https://doi.org/10.56093/ijans.v96i7.177311

Keywords:

Alpha-lactalbumin, beta-lactoglobulin, buffalo breeds, mammary epithelial cells, milk protein transcript, real-time PCR

Abstract

The present study examined the relative expression of beta-lactoglobulin (LGB) and alpha-lactalbumin (LAA) genes in milk-derived primary bovine mammary epithelial cells (pBMEC) of two Indian buffalo breeds — Surti and Mehsani — at day 15 and 60 postpartum, and correlated gene expression with milk yield and composition traits. Milk samples were collected non-invasively and repeatedly from the same 13 Surti and 13 Mehsani buffaloes, categorized into four groups: S15, S60, M15 and M60. pBMEC were purified from fresh milk using an immunomagnetic-bead based separation technique employing cytokeratin 8 antibody and real-time PCR was performed using GAPD as the reference gene. Mean relative expression of the LGB gene was significantly lowered with advancement of lactation in Surti buffaloes while it remained steady in Mehsani buffaloes, indicating breed-specific regulation. A significant between-breed difference in LGB gene expression was observed at day 60 postpartum, with Mehsani buffaloes showing 16.67 folds higher expression than Surti buffaloes, consistent with their higher milk protein content. Mean relative expression of the LAA gene did not differ significantly within or between breeds throughout the study period. The LAA gene transcript consistently constituted the predominant whey protein transcript fraction across all groups. Breed-specific regulation of LGB gene expression in Indian buffaloes, and its association with milk yield in Mehsani buffaloes at early lactation, has implications for molecular marker-assisted selection for improved milk production in these breeds.

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