Assessment of Hematobiochemical and Stress Responses in Buffaloes (Bubalus bubalis) Undergoing Single and Double Stage Diaphragmatic Herniorrhaphy

Dr Neeraj Arora; sreekanth P; sandeep potlitya; deepak kumar tiwari; rajesh kumar; ankit kumar; raveena kumari

doi:10.56093/ijans.v96i7.178449

Authors

Dr Neeraj Arora Lala lajpat rai university of veterinary and animal sciences hisar
sreekanth P
sandeep potlitya
deepak kumar tiwari
rajesh kumar
ankit kumar
raveena kumari

https://doi.org/10.56093/ijans.v96i7.178449

Keywords:

Diaphragmatic herniorrhaphy, Haematobiochemical,Oxidative stress

Abstract

The present study was aimed to evaluate and compare the hematobiochemical alterations and stress responses in twelve female Murrah buffaloes diagnosed with diaphragmatic hernia and treated using two different surgical approaches. The animals were divided into Group I (n=6), subjected to single-stage diaphragmatic herniorrhaphy without laparo-rumenotomy, and Group II (n=6), subjected to double-stage repair involving laparo-rumenotomy followed by diaphragmatic herniorrhaphy. Haematological, biochemical, oxidative stress and inflammatory parameters were evaluated during the preoperative and postoperative periods. Haematological examination revealed changes in haemoglobin, packed cell volume, total leukocyte count, neutrophil count and lymphocyte count in both groups, with more pronounced leukocytosis, neutrophilia and lymphopenia in Group II. Among biochemical parameters, alterations were observed in alanine aminotransferase, blood urea nitrogen, blood glucose, sodium and chloride, while most other indices remained comparable. Stress and inflammatory markers showed progressive changes, with increased serum cortisol, malondialdehyde and C-reactive protein, along with decreased total antioxidant capacity in both groups. Group II showed higher cortisol, malondialdehyde and C-reactive protein levels and lower total antioxidant capacity, suggesting greater inflammatory, oxidative and physiological stress. The study concluded that buffaloes undergoing single-stage diaphragmatic herniorrhaphy showed less pronounced hematobiochemical and stress alterations than those undergoing double-stage repair.

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