Impact of initiating a PGF2α-GnRH fixed-time AI protocol at the late luteal phase on reproductive performance of repeat-breeder crossbred dairy cattle

S P S Ghuman; M Honparkhe; Jagir Singh

doi:10.56093/ijans.v82i8.23001

Authors

S P S Ghuman Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
M Honparkhe Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
Jagir Singh Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India

https://doi.org/10.56093/ijans.v82i8.23001

Keywords:

Cattle, Conception rate, GnRH, PGF2Î±, Repeat-breeder

Abstract

The objectives of this study were to document ovarian and endocrine responses as well as pregnancy establishment following the treatment of repeat-breeder crossbred dairy cattle with a prostaglandin F₂a-gonadotropin releasing hormone (PGF₂a-GnRH) fixed-time artificial insemination (AI) protocol at the late luteal phase. Cattle (15) were administered PGF₂a (500 µg cloprostenol) on day 16 of the estrous cycle, followed by GnRH analogue (20 µg buserelin) 5 days later. About 24 h after GnRH, AI was carried out in all the cattle. Transrectal ovarian ultrasonography was carried out at the time of PGF₂a administration, at AI and on days 5, 16 and 21 post-AI. Plasma progesterone was assayed with solid- phase radioimmunoassay. All the cattle responded to treatment with a luteolytic dose of PGF₂a. On the day of AI, large follicles of 15.94±0.60 (12.7–22.0) mm diameter were observed in all the cattle. The presence of active luteal profile on day 5 post-AI confirmed the occurrence of ovulation in all the cattle. Post-treatment first service conception rate was appreciable (66.7%) in cattle with history of repeat breeding. Repeat-breeder cattle failing to conceive after hormonal treatment had suprabasal plasma progesterone on the day of AI in comparison to their non-conceiving counterparts. In conclusion, initiation of PGF₂a-GnRH protocol based fixed-time AI protocol at the late luteal phase is highly successful for inducing ovulation in all the repeat-breeder cattle, and yielded an appreciably high conception rate.

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