Quantitative estimation of some macro- and micro-minerals in follicular fluid of indigenous pigs
91 / 53
Keywords:
Follicle, Follicular fluid, Mineral, PorcineAbstract
In the present study, characteristics of porcine follicular fluid (FF) harvested from different sized ovarian follicles and developmental competences of enclosed oocytes in relation to their sizes were investigated. It has been observed that some mineral components of FF like Ca, P, Cu and Fe significantly increased as the follicles increased in size, while only Li, which is first reported here in porcine FF decreased significantly with increase in follicular size. Therefore, it may enhance more success in reproduction in this species, which may improve the certain macro and micro mineral level in follicular fluid of oocytes.
Downloads
References
Abd Ellah M R, Hussein H A and Derar D R. 2010. Ovarian Follicular Fluid Constituents in Relation to Stage of Estrus Cycle and Size of the Follicle in Buffalo. Veterinary World 3(6): 263– 67.
Agarwal R and Henkin R. 1985. A simple method of simultaneous estimation of zinc and copper in erythrocytes. Biological Trace Element Research 7(4): 199–208.
Amador E and Urban J. 1977. Simplified serum phosphorus analysis by contentious flow UV spectrophotometry. Clinical Chemistry 18: 60.
Bagavandoss P, Midgley A R and Wicha M. 1983. Developmental changes in the ovarian follicular basal lamina detected by immunofluorescence and electron microscopy. Journal of Histochemistry and Cytochemistry 31: 633–40.
Bagger P V, Byskov A G, Christiansen M D, Bang L and Mortensen L. 1993. Lithium stimulates the first meiotic division in mouse oocytess. Acta Obstetricia et Gynecologica Scandinavica 72: 514–19.
Belle R, Mulner-lorillon O, Marot J and Ozon R. 1986. A possible role of Mg2 Ions in the induction of meiotic maturation of xenopus oocyte. Cell Differentiation 19: 253–61.
Boland M P. 2003. Trace minerals in production and reproduction in dairy cows. Advanced Dairy Technology 15: 319–20.
Gerard N, Loiseau S, Duchamp G and Seguin F. 2002. Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance. Reproduction 124: 241–48.
Guraya S S, Parminder K and Sharma R K. 1991. Histochemical and biochemical studies on esterase activity in the rat ovary. European Journal of Morphology 29(3): 161–72.
Kalmath G P. 2000. Biochemical analysis of ovarian antral follicular fluid in buffaloes MVSc. thesis, University of Agricultural Sciences, Bangalore. Indian Journal of Reproduction and Fertility 82: 813–25.
Knudsen J F, Litkowski L J, Wilson T L, Guthrie H D and Batta S K. 1979. Follicular fluid electrolytes and osmolality in cyclic pigs. Reproduction and Fertility 57(2): 419–22.
Leibfreld L and First N L. 1979. Effects of divelent cations on in vitro maturation of bovine oocytes. Journal of Experimental Zoology 210: 575–80.
Marketa S and Rozinek Petr J. 2002. Labelling of bound calcium in pig egg ultra structure. 2nd Annual Meeting of CSMS, Vranovska Ves Tema Microscopy 59: 14–15.
Mishra O P, Pandey J N and Gawande P G. 2003 Study on biochemical constituents of caprine ovarian follicular fluid after superovulation. Asian-Australian Journal of Animal Sciences 16: 1711–15.
Schwartzenbach G, Biedermann W and Bangerter F. 1956. Helv. Chim Acta 29: 811.
Sangha G K, Sharma R K and Guraya S S. 1993. Distribution of trace elements in blood and ovary during estrous cycle and pregnancy in house rat. Indian Journal of Animal Sciences 63(2): 142–45.
Sharma R K and Sharma M. 1997. Physiology perspectives of Copper: A review. Indian Journal of Experimental Biology 35: 696–713.
Siedel J, Wahlefeld A H and Ziegenhorn J. 1984. A new iron ferrozinc- reagent without deproteinisation. Clinical Chemistry 30: 975.
Snedecor G W and Cochran W G. 1994. Statistical Methods. 8th edn. Kolkata, India, Oxford and IBH Publishing Co. Tietz N W. 1976. Electrolytes. Fundamentals of Clinical Chemistry. 3 edn, pp. 873–944. Philadelphia, W B Saunders Co.
Vonknoring L, Oriland L, Perris C and Punebery S. 1976. Lithium RBC/PLASMA ratio in sub groups of patients with effective disorders. Neuropsychobiology 2: 74–80.
Wise T. 1987. Biochemical analysis of bovine follicular fluid: albumin, total proteins, lysosomal enzymes, ions, steroid and ascorbic acid contents in relation to follicular size rank, atresia classification and day of oestrous cycle. Journal of Animal Science 64(4): 1153–69.
Whitmore D N and Evans D I K. 1964. An automated method for serum magnesium estimation. Journal of Clinical Pathology 17: 644–48.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.