Buffalo umbilical cord blood collection and hematological comparison with peripheral blood of new born calf and its dam

Jarnail Singh; Vijay Singh; Anita Mann; J K Singh; Jerome A; S K Sarkar; J S Dhuan; P S Yadav

doi:10.56093/ijans.v82i8.23032

Authors

Jarnail Singh Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
Vijay Singh Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
Anita Mann Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
J K Singh Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
Jerome A Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
S K Sarkar Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
J S Dhuan Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
P S Yadav Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India

https://doi.org/10.56093/ijans.v82i8.23032

Keywords:

Buffalo, Cord blood, Hematology, Peripheral blood

Abstract

The present study was carried out to explore the feasibility of umbilical cord blood collection and to compare its hematology with peripheral blood of new born calf and the adult buffalo. Jugular blood samples (7) were collected from new born calf and its dam and umbilical cord blood from passed placenta immediately after placental expulsion. These blood samples were analyzed for 22 hematological parameters. Statistical analysis of 22 hematological parameters from all the 3 sources indicated that 7 parameters, viz. WBC, LYM, MON, NEU, HGB, PLT, PCT % of calf and 12 parameters, viz. WBC, LYM, MON, NEU, EOS, BAS, LY %, NE %, HCT %, MCV, MCH, PCT % of adult blood, respectively, were significantly higher than the cord blood, and 3 parameters, viz. RBC, RDWc %, and MO % of adult blood were significantly lower than that of cord blood. A significant difference between cord blood and calf blood with respect to 7 parameters, viz. WBC, LYM, MON, NEU, HGB, PLT, PCT % proves that cord blood and calf blood are not exactly same regarding their cellular content. This difference in hematological parameters of cord blood may be helpful in immunomodulation so that it can be used in transplantation and represent the neonatal defense system against the earliest encountered pathogens.

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References

Ballen K, Broxmeyer Hal E, McCullough J, Piaciabello, W, Rebulla P, Verfaillie, Catherine M and Wagner John E. 2001. Current status of cord blood banking and transplantation in the United States and Europe. Biology of Blood and Marrow Transplantation 7: 635–45.

Bartholomew S, Owens S D, Ferraro G L, Carrade D D, Lara D J, Librach F A, Borjesson D L and Gluppo L D. 2009.Collection of equine cord blood and placental tissues in 40 thoroughbred mares. Equine Veterinary Journal 41 (8): 724–28.

Chandra K N, Prasad V G N V, Narasimha Reddy Bhaskar CH E, Pandiyan V, Guru D V and Murlinath E. 2008. Haematology of graded murrah buffaloes in the coastal region of Andhra Pradesh (India). Buffalo Bulletin 27 (3): 236–39.

Khajuria R R and Razdan M N. 1966. Haematological studies in dairy cattle. 2. Leucocytic picture under different physiological states. The Indian Veterinary Journal 43 : 886.

Sharma M C, Pathak N N, Verma R P, Hung N N, Cu N V, Lien N H, An D T, Mai H V and Vuc N V. 1985. Normal haematology of Murrah buffaloes of various ages in the agroclimatic conditions in Viet Nam. Indian Veterinary Journal 62 : 383.

Simon K J and Jacob A. 1961. Studies on constituents of blood of buffaloes. Part I Cellular elements and haemoglobin. Indian Veterinary Journal 38: 183–88.

Thangraj T M, Sheshagiri V N, Krishanan A R and Venkata Swami V. 1979. Haematological changes in neonate of Bubalus Bubalis. Indian Journal of Dairy Science 32: 240.