Evaluation of serum antibody responses of Madras Red sheep to  excretory/secretory antigen of Haemonchus contortus

S Arunkumar; S Abdul Basith; Gomathinagayam Gomathinagayam

doi:10.56093/ijans.v82i9.23645

Authors

S Arunkumar Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
S Abdul Basith Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
Gomathinagayam Gomathinagayam Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India

https://doi.org/10.56093/ijans.v82i9.23645

Keywords:

Antibody, Excretory /Secretory antigen, ELISA, Haemonchus contortus, Sheep

Abstract

Excretory/secretory (E/S) antigen from Haemonchus contortus was isolated by culturing live adult worms in RPMI 1640 medium at 37° C for 24 h and the culture supernatant was used as antigen. Immuno blot analysis revealed that the E/S antigen showed 5 reactive bands at 24, 29, 46, 66 and 93 kDa. On immunization trial, sheep were immunized with 500 mg of E/S antigen along with montanide as adjuvant on day 0, 30 and 60 intramuscularly. Further, the assessment of serum antibody levels in immunized sheep was made by enzyme linked immuno sorbent assay (ELISA). Based on absorbance values, it was observed that the serum antibody levels were significantly higher up to 20 weeks post immunization in immunized sheep compared to control sheep.

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