Biochemical characterization of foot-and-mouth disease virus

Rita Nath; Ram Lakhan Prasad; Sankar Sarma

doi:10.56093/ijans.v82i9.23654

Authors

Rita Nath Birsa Agricultural University, Ranchi, Jharkhand 834 006 India
Ram Lakhan Prasad Birsa Agricultural University, Ranchi, Jharkhand 834 006 India
Sankar Sarma Birsa Agricultural University, Ranchi, Jharkhand 834 006 India

https://doi.org/10.56093/ijans.v82i9.23654

Keywords:

FMD, Mobility, Molecular weight, Protein, Vaccine

Abstract

The present study was carried out to determine the molecular weight and mobility of the proteins present in the FMD virus. FMD trivalent vaccine and FMD infected serum samples were used for the study. The standard SDS-6H of known proteins was used for calculating the molecular weight of different proteins.The molecular weight of the FMD viral proteins 1 and 2 were 101 and 62 K Da and mobilities were 0.268 and 0.521 respectively. Seven distinct bands segregrated in control and FMD affected serum samples. The bands in the control sample from the top of the gel were identified as g globulin, ß globulin, unidentified protein, heptoglobin, albumin, a₂ globulin and unidentified protein. Their molecular weights were 150, 140, 120, 100, 60, 48 and 33 K Da and mobilities were 0.094, 0.126, 0.186, 0.268, 0.536, 0.631 and 0.805 respectively. In the FMD affected sample, g –globulin was undetectable but an extra band was noticed in the infected serum sample which was identified as the FMD viral protein.

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