A 28KDa recombinant outer membrane protein of Brucella melitensis downregulates  type-1 immune response  in mice

P Kaushik; D K Singh; A K Tiwari; S Dayal; G Shukla; A Quasim; H Singha; B Kumar; P Chaudhury

doi:10.56093/ijans.v82i9.23655

Authors

P Kaushik Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
D K Singh Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A K Tiwari Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
S Dayal Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
G Shukla Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A Quasim Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
H Singha Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
B Kumar Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
P Chaudhury Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v82i9.23655

Keywords:

Brucella, Omp28, Type-1 immune response

Abstract

A 28 kDa recombinant outer membrane protein (r-omp28) of Brucella melitensis was evaluated for the induction of immune response in mice. The study demonstrated the downregulation of type-1 immune response in mice vaccinated with r-omp28 of B. melitensis. The level of IgG2a was observed significantly lower than IgG1 in mice immunized with r-omp28. The cytokine profile and proliferation of cells was characteristic of Th2 type as we detected significantly high level of IL-10 but no or significantly low level of IL-2 and IFN-g. Further, the production of gamma-interferon (IFN-g) was downregulated by r-omp28. Therefore, it implies that B. melitensis r-omp28 might down-regulates type-1 immune response in vaccinated mice.

Downloads

Download data is not yet available.

References

Al-Mariri A, Tibor A, Mertens P, DeBolle X, Michel P, Godefroid J, Walravens K and Letesson J J. 2001. Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant. Infection Immunity 69: 4816–22.

Bhattacharjee A K, Izadjoo M J, Zollinger W D, Nikolich M P and Hoover D L. 2006. Comparison of protective efficacy of subcutaneous versus impanasal immunization of mice with a Brucella melitensis lipopolysaccharide subunit vaccine. Infection and Immunology 74: 5820–25.

Cassataro J, Velikovsky C A, Barrera S. et al. 2005. A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against Brucella melitensis and Brucella ovis infection by eliciting a specific cytotoxic response. Infection and Immunology 73:6537–46.

Cassataro J K, Pasquevich L, Bruno J C, Wallach C A and Fossati P C. 2004. Antibody reactivity to Omp31 from Brucella melitensis in human and animal infections by smooth and rough Brucellae. Clinical Diagnosis and Laboratry Immunology 11: 111–14.

Chaudhuri P, Kumar S V, Prasad R, Srivastava S K and Yadav M P. 2005. Cloning and sequencing of 28 kDa outer membrane proein gene of Brucella melitensis Rev 1. Indian Journal of Experimental Biology 43: 838–40.

Coban C, Ishii K J, Stowers A W, Keister D B, Klinman D M and Kumar N. 2004. Effect of CpG oligodeoxynucleotides on the immunogenicity of Pfs25, a Plasmodium falciparum transmissionblocking vaccine antigen. Infection and Immunology 72: 584–88.

Corbel M J. 1997. Vaccines against bacterial zoonoses. Journal of Medical Microbiology 46: 267–69.

Diptee M D, Adesiyun A A, Asgarali Z, Campbell M and Fosgate G T. 2005. Evaluation of cell-mediated immune responses and bacterial clearance in 6–10 month old water buffalo (Bubalus bubalis) experimentally vaccinated with four diseases of commerical B. abortus strain RB51 vaccine. Veterinary Immunology. Immunopathology 106: 209–20.

Fernandes D M and Baldwin C L. 1995. Interlukin-10 downregulates protective immunity to B. abortus. Infection and Immunology 63: 1130–33.

Huang L, Krieg A M, Eller N and Scott D E. 1999. Induction and regulation of Th1-inducing cytokines by bacterial DNA, lopopolysaccharide, and heat-inactivated bacteria. Infection and Immunology 67: 6257–63.

Jacques I, Verger J M, Laroucau K, Grayon M, Vizcaino N, Peire A, Cortade F, Carreras F and Guilloteau L A. 2007. Immunological responses and protective efficacy againt B. melitensis induced by bp 26 and Omp31 B. melitensis Rev.1 deletion mutants in sheep. Vaccine 25: 794–805.

Jegerlehner A, Maurer P, Juliana B, Hinton H J, Kopf M and Bahmann M F. 2007. TLR9 signialing in B cells determines class switch recombination to IgG2a. Journal of Immunology 178: 2415–20.

Kaushik P, Singh D K, Kumar S V, Tiwari A K, Shukla G, Dayal S and Chaudhuri P. 2010. Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanyed with CpG oligonucleotides. Veterinary Research and Communication 34: 119–32.

Mallick A I, Singha H, Chaudhuri P, Nadeem A, Khan S A, Dar K A, Owais M. 2007. liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. Vaccine 25: 3692–3704.

Mosmann T R. 1983. A rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assay. Journal of Immunology Method 65: 55–63.

Splitter G, Oliveira S, Carey M, Miller C, Ko J and Covert J. 1996. T lymphocyte mediated protection against facultative intracellular bacteria. Veterinary Immunology and Immunopathology 54: 309–19.

Vemulapalli R, He Y, Cravero S, Sriranganathan N, Boyle S M and Schurig G G. 2000. Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51. Infection and Immunology 68: 3286–89.