Recombinant chicken anaemia virus viral protein 1 gene expression in insect cell system

KARTHIKA T; MANOHARAN S; JAYALAKSHMI E T; KUMANAN K

doi:10.56093/ijans.v82i10.24277

Authors

KARTHIKA T Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
MANOHARAN S Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
JAYALAKSHMI E T Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
KUMANAN K Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

https://doi.org/10.56093/ijans.v82i10.24277

Keywords:

Baculovirus, Chicken anaemia virus, Cloning, Insect cells, Protein expression, Viral protein 1

Abstract

CAV specific PCR positive tissue samples of Namakkal region available in the Department of Animal Biotechnology, Madras Veterinary College, were used in this study. A multiple host expression vector pTriEx 1.1 Neo was selected to clone the CAV VP1 gene (1353 bp) and transformed into BL21 (DE3) strain of E. coli and confirmed. The CAV VP1 recombinant pTriEx vector was co- transfected to Bacvector triple cut virus DNA with Sf9 insect cells. The plaques formed by CAV VP1 recombinant baculovirus were purified and propagated for protein expression. The recombinant CAV VP1 protein expression was confirmed by indirect FAT and SDS-PAGE analysis followed by western blot analysis using specific serum.

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Author Biographies

KARTHIKA T, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
MANOHARAN S, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
JAYALAKSHMI E T, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
KUMANAN K, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

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