Multiplex PCR for rapid detection of Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae in subclinical mastitis milk

BIBEK RANJAN SHOME; MANI BHUVANA; SUSWETA DAS MITRA; NATESAN KRITHIGA; RAJESWARI SHOME; DHANIKACHALAM VELU; APALA BANERJEE; PINAKI PRASAD SENGUPTA; HABIBAR RAHMAN

doi:10.56093/ijans.v82i10.24279

Authors

BIBEK RANJAN SHOME Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
MANI BHUVANA Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
SUSWETA DAS MITRA Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
NATESAN KRITHIGA Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
RAJESWARI SHOME Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
DHANIKACHALAM VELU Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
APALA BANERJEE Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
PINAKI PRASAD SENGUPTA Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
HABIBAR RAHMAN Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India

https://doi.org/10.56093/ijans.v82i10.24279

Keywords:

Bovine mastitis, Diagnosis, Mastitis, Multiplex PCR, Streptococci

Abstract

To improve mastitis diagnosis and achieve rapid, specific, reliable and cost effective test, a multiplex PCR for simultaneous detection and differentiation of major streptococcal species, viz. Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae was developed. Evaluation with 24 ATCC strains and 606 strains comprising streptococci (84) and staphylococci (522) showed the assay to be highly accurate. The threshold of detection of the mPCR assay was 10fg of genomic DNA and < 102 CFU ml-1. Assessment of 115 milk samples collected from subclinically infected herd, showed mPCR assay to be more efficacious than culture method. Identification of Streptococcus species using this assay will be crucial to determine prevalence of Streptococcus in a herd. This will facilitate early diagnosis, treatment and control of the rate of infection at farm level. The assay can be implemented for routine monitoring of herd health and can be of great value for promoting prevention of streptococcal mastitis.

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Author Biographies

BIBEK RANJAN SHOME, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
MANI BHUVANA, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
SUSWETA DAS MITRA, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
NATESAN KRITHIGA, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
RAJESWARI SHOME, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
DHANIKACHALAM VELU, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
APALA BANERJEE, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
PINAKI PRASAD SENGUPTA, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India
HABIBAR RAHMAN, Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru, Karnataka 560 024 India

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