Antibacterial activity of successive extracts of some medicinal plants against field isolates of Pasteurella multocida
302 / 83
Authors
-
N P USHA
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
-
K J BIBU
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
-
SUBIN JOSE
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
-
A M C NAIR
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
-
G KRISHNAN NAIR
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
-
N D NAIR
Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala 673 576 India
Keywords:
Disc diffusion, Microtitre plate dilution, Pasteurella multocida, Phytochemical analysisAbstract
The outbreak of duck pasteurellosis caused by avian strains of Pasteurella multocida, which occur during monsoon period, is a serious problem with high mortality and morbidity affecting younger age groups. Medicinal plants reported to have antibacterial action were tested for their efficacy against Pasteurella multocida by in vitro methods. Successive extraction of whole plant materials of Leucas aspera, Vernonia cineria and Ocimum sanctum, leaves of Syzigium cumini, and bark of Polyalthia longifolia were performed with petroleum benzine, choloroform, acetone, methanol and water, and were tested for their antibacterial effect using microtitre plate technique (to estimate the minimum inhibitory concentration, MIC) and disc diffusion method (to estimate the zone of inhibition). The results from the above in vitro assay revealed the efficacy of different plant successive extracts against duck pasteurellosis.
Downloads
References
Barrow C I and Feltham R K A. 1993. Cowan and Steel’s Manual for Identification of Medical Bacteria. 3rd edn. Cambridge University Press.
Bauer A W, Kirby W M, Sherris J C and Turck M. 1966. Antibiotic susceptibility testing by standardized single disc method. American Journal of Clinical Pathology 45: 493–96.
Bibu K J, Joy A D and Mercey K A. 2010. Therapeutic effect of ethanolic extract of Hygrophila spinosa T. Anders on gentamicin-induced nephrotoxicity in rats. Indian Journal of Experimental Biology 48: 911–17.
Bylka W, Matlawska I and Pilewski N.A. 2004. Natural flavonoids as antimicrobial agents. Journal of American Nutraceutical Association 7: 24–31.
Chanda S and Nair R. 2010. Antimicrobial activity of Polyalthia longifolia (Sonn.) Thw.var. Pendula leaf extracts against 91 clinically importantpathogenic microbial strains. Chinese Medicine 1: 31–38.
Daferera D J, Ziogas B N and Polissiou M G. 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Protection 22: 39–44.
Gowri S S and Vasantha K. 2010. Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) leaves extracts. International Journal of PharmTech Research 2: 1569– 73.
Harborne J B. 1991. Phytochemical Methods-Guide to Modern Techniques of Plant Analysis. 2nd edn. Chapman and Hall, India. Jasmine R, Selvakumar B N and Daisy P. 2011. Investigating the mechanism of action of terpenoids and the effect of interfering substances of an Indian medicinal plant extract demonstrating antibacterial activity. International Journal of Pharmaceutical Sciences and Research 2(2): 19–24.
Mishra P and Mishra S. 2011. Study of antibacterial acitivity of Ocimum sanctum extract against gram positive and gram negative bacteria. American Journal of Food Technology 6: 336– 41.
Okoro I O, Osagie A, Asibor E O. 2010. Antioxidant and antimicrobial property of polyphenols from ethnomedicinal plants of Nigeria. African Journal of Biotechnology 9(20) 2989– 93.
Oliveira G F, Furtado N A J C, Filho A A S, Martins C H G, Bastos J K, Cunha W R and Silva M L A. 2007. Antimicrobial activity of Syzygium cumini (Myrtaceae) leaves extract. Brazilian Journal of Microbiology 38: 381–84.
Omulokoli E, Khan B and Chhabra S C. 1997. Antiplasmodial activity of four Kenyan medicinal plants. Journal of Ethnopharmacology 56: 133–37.
Sheena N, Ajith T A, Mathew T and Janardhanam K K. 2003. Antibacterial activity of three macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus occurring in South India. Pharmaceutical Biology 41(8): 564–67.
Sultana B, Anwar F and Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14: 2167–80.
Usha N P, Bibu K J, Subin Jose, Jayakumar KM, Nair N D, Nair A M C and Krishnan Nair G. 2010. Therapeutic efficacy of Polyalthia longifolia leaves extract against Pasteurella multocida in ducklings. Indian Journal of Veterinary Medicine 30: 20–24.
Walter C, Shinwari Z K, Afzal I and Malik R N. 2011. Antibacterial activity in herbal products used in Pakistan. Pakistan Journal of Botany 43: 155–62.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Animal Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
