Genetic characterisation of two humped camel of India (Camelus bactrianus)

PRIYANKA BANERJEE; JYOTI JOSHI; UPASNA S; NAZIR GANAI; R K VIJH

doi:10.56093/ijans.v82i10.24308

Authors

PRIYANKA BANERJEE National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
JYOTI JOSHI National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
UPASNA S National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
NAZIR GANAI National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
R K VIJH National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India

https://doi.org/10.56093/ijans.v82i10.24308

Keywords:

Camelus bactrianus, Genetic characterization, Microsatellite

Abstract

Microsatellite data was generated on 17 bactrian camels using 27 markers. The data analysis revealed a fair degree of heterozygosity existing in these bactrian camel which are very few in number (149). The mean heterozygosity and effective number of alleles were 0.56 and 3.89 respectively. The F statistics also revealed normal rate of inbreeding in this stock. This was attributed to free flow of bactrian camel germplasm between valley and Tibetan plateau till recent times. The population constancy was also established using inter and intralocus tests. Effective conservation measures are suggested owing to small number of individuals and systematic mating plan is advocated.

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Author Biographies

PRIYANKA BANERJEE, National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
JYOTI JOSHI, National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
UPASNA S, National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
NAZIR GANAI, National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India
R K VIJH, National Bureau of Animal Genetic Resources Karnal, Haryana 132001 India

References

HongWei Gao, Jing Wang, He Jun Xia, Chen Lu Yong, Ji R and Meng He. 2009. Analysis of genetic diversity and evolution in the Camelus Bactrianus using SSR markers. Journal of Shanghai Jiaotong University – Agricultural Science 27: 89–95.

Jianlin H, Ochieng J, Lkhagva B and Hanotte O. 2004. Genetic diversity and relationship of domestic Bactrian camels (Camelus bactrianus) in China and Mongolia. Journal of Camel Practice and Research 11(2): 97–99.

Lang K D M, Vang Y and Plante Y. 1996. Fifteen polymorphic dinucleotide microsatellites in llamas and alpacas. Animal Genetics 27: 293.

Luikart G, Cornuet J M and Allendorf F W. 1998. Temporal changes in allele frequencies provide estimates of population bottleneck size. Conservation Biology 89: 238–47.

Maruyama T and Fuerst P A. 1985. Population Bottlenecks and Non-Equilibrium Models in Population Genetics. 2. Number of Alleles in a Small Population that was formed by a Recent Bottleneck. Genetics 111: 675–89.

Mason. 1984. Evolution of Domesticated Animals. Longmam Group Ltd, Essex CM20, 2 JE, England, Chapter 13: 106 – 115.

Mishra Bina, Tantia M S, Bharani Kumar S T, Mishra B P and Vijh R K. 2009. Genetic relationship among camels of India based on microsatellite data. Indian Journal of Animal Sciences 79 (4): 399–405.

Piry S, Luikart G and Cornuet J M. 1999. BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. Journal of Heredity 90: 502–503.

Olivier Langella. 1999. Populations - A population genetics software, 1.2.30 Copyright (C) CNRS UPR9034. http://www.bioinformatics.org/~tryphon/populations/.

Raymond M and Rousset F. 1995. GENEPOP (version 3.4): Population genetics software for exact test and ecumenicism.

Journal of Heredity 86: 248–249. ftp://ftp.cefe.cnrs-mop.fr/pub/ pc/msdos/genepop.

Reich D E and Goldstein D B. 1998. Genetic evidence for a Paleolithic human population expansion in Africa. Proceedings of the National Academy of Sciences of the United States of America 95: 8119–23.

Reich D E, Feldman M W and Goldstein D B. 1999. Statistical properties of two tests that use multilocus data sets to detect population expansions. Molecular Biology and Evolution 16: 453–66.

Robertson A and Hill W G. 1984. Deviations from Hardy-Weinberg proportions: sampling variances and use in estimation of inbreeding coefficients. Genetics 107: 703–718.

Saitou Naruya and Masatoshi Nei. 1987. The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406–25.

Sambrook J and Russell D W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Tree explorer version 2.12 downloadable from (http://en.bio- soft.net/tree/TreeExplorer.html).

Vijh R K, Tantia M S, Mishra B and Bharani Kumar S T. 2007. Genetic diversity and differentiation of dromedarian camel of India. Animal Biotechnology 18(2): 81–90.

Weir B S and Cockerham C C. 1984. Estimating F-statistics for the analysis of population structure. Evolution 38: 1358– 70.

Yeh F, Boyle C, Rongcai T, Ye Z Y and Xian J M. 1999. POPGENE: ver 1.31. A Microsoft window based freeware for population genetic analysis. University of Alberta, Edmonton. http://www.ualberta.ca/-fyeh/fyeh.