Application of loop mediated isothermal amplification (LAMP) assay as an alternative diagnostic test for rapid tuberculosis diagnosis in limited resource setting

SONAM DOLKER; RISHENDRA VERMA; CHANDAN PRAKASH; ASHABREE SHAMAL

doi:10.56093/ijans.v82i11.25129

Authors

SONAM DOLKER Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
RISHENDRA VERMA Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
CHANDAN PRAKASH Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ASHABREE SHAMAL Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v82i11.25129

Keywords:

Mycobacterium tuberculosis, LAMP, TB diagnosis

Abstract

Diagnostic potentiality of loop mediated isothermal amplification (LAMP) assay with human sputum samples was compared with smear microscopy, culture and PCR in providing rapid and accurate TB diagnosis. Practical application of this assay was emphasized as a supplementary diagnostic test with acid fast smear microscopy for controlling tuberculosis in limited resource setting particularly India. Sputum samples (261) from TB suspected patients were collected, processed and subjected to smear microscopy, culture, PCR and LAMP assay. These 4 diagnostic methodologies were compared in terms of sensitivity, specificity, and rapidity and user friendliness. Sensitivity of LAMP, PCR and microscopy were 97.47, 97.47, and 61.61%, respectively, and specificity of LAMP, PCR and microscopy were 60.31, 74.60 and 100% respectively. Higher sensitivity of diagnostic test will be more useful in high TB endemicity area to cover most of TB patients under National Tuberculosis Control Programmae. This LAMP assay may be a viable alternative of costly PCR diagnosis. This test may be adopted as supplementary diagnostic test with acid fast smear microscopy for routine diagnosis of human TB.

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Author Biographies

SONAM DOLKER, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
RISHENDRA VERMA, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
CHANDAN PRAKASH, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ASHABREE SHAMAL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

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