Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro

LI XIANGCHEN; YAO YAXIN; WANG YIPENG; LI HEPING; GUAN WEIJUN; MA YUEHUI

doi:10.56093/ijans.v82i11.25133

Authors

LI XIANGCHEN Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
YAO YAXIN Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
WANG YIPENG Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
LI HEPING Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
GUAN WEIJUN Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
MA YUEHUI Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China

https://doi.org/10.56093/ijans.v82i11.25133

Keywords:

Bovine, Embryos, Oocyte, Vitrification

Abstract

The effectiveness of existing procedures based on viable embryos per oocyte frozen remains low compared to use of fresh oocytes in cattle, and the probable causes of the freezing-induced reduction in oocyte developmental competence include chromosome, cytoskeleton disruption, or even the choice of the frozen stage of the oocytes. In the present study, after vitrification, the survival rate of GV and MI oocytes were significantly lower than those in MII. The vitrifid GV oocyte showed some chromosomes dispersed and less condensed, The vitrifid MI oocytes with “Fading’’ and discontinuous staining pattern were as having an abnormal microfilament distribution. The stage of GV and MI, both cleavage rate and blastocyst formation rate were reduced significantly. Therefore, the cryopreservation of GV or MI oocytes seems could not instead of the MII oocytes.

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Author Biographies

LI XIANGCHEN, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
YAO YAXIN, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
WANG YIPENG, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
LI HEPING, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
GUAN WEIJUN, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China
MA YUEHUI, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193 PR China

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