Effect of superovulatory treatment on serum estradiol -17ß and progesterone concentration and its correlation with embryo recovery in progesterone concentration and its correlation with embryo recovery in mithun cows

CHAMPAK BARMAN; ANUBHA BARUAH; B K SARMAH; K K BARUAH; B C SARMAH; B C DEKA; A DUTTA; P CHAKRAVARTY; M H KHAN; SURESH KUMAR; P K BHARTI

doi:10.56093/ijans.v82i11.25137

Authors

CHAMPAK BARMAN Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
ANUBHA BARUAH Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B K SARMAH Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
K K BARUAH Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B C SARMAH Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B C DEKA Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
A DUTTA Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
P CHAKRAVARTY Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
M H KHAN Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
SURESH KUMAR Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
P K BHARTI Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India

https://doi.org/10.56093/ijans.v82i11.25137

Keywords:

Exogenous hormone, Mithun cows, Oestrous cycle, Superovulation

Abstract

The present experiment was conducted to observe the effects of superovulatory treatments on serum estradiol-17ß and progesterone concentration and its correlation with embryo recovery in mithun cows (Bos frontalis). Healthy cyclic mithun cows (20) between second and fifth lactation were randomly divided into 4 groups (n=5) viz. A, B, C and D. Animals were maintained under intensive system of management at NRC on Mithun, Nagaland. Each animal of groups A, B, C and D was treated, respectively, with 400 mg FSH, 300 mg FSH, 400 mg FSH + 1500 IU hCG and 300 mg FSH + 1500 IU hCG in divided and reducing doses during 4 days starting on day 11 of estrous cycle. Prostaglandin F₂a was injected intramuscularly on day 13 of estrous cycle after sixth injection of FSH. hCG was injected intramuscularly 12 h post oestrus. Results revealed that serum oestradiol-17ß was higher in cows treated with 400 mg FSH compared to 300 mg that became significant on day 4 of treatment in group C (10.20±0.34 pg/ml) than group B (9.25±0.35 pg/ml). Significantly higher serum oestradiol was recorded at induced oestrus in all the treatment groups.

Serum progesterone level decreased to a significant level on the day of induced oestrus in all the 4 groups irrespective of treatment. Serum oestradiol-17ß at induced oestrus had positive correlations with the number of total embryos recovered. It may be concluded that the serum estradiol and progesterone concentration are good indicator of embryo recovery in superovulated mithun cows.

Downloads

Download data is not yet available.

Author Biographies

CHAMPAK BARMAN, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
ANUBHA BARUAH, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B K SARMAH, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
K K BARUAH, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B C SARMAH, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
B C DEKA, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
A DUTTA, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
P CHAKRAVARTY, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
M H KHAN, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
SURESH KUMAR, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
P K BHARTI, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India

References

Arosh A J, Sakhila K, Devanathan T G, Kathiresan D. 2001. Progesterone, oestradiol, luteinizing hormone and follicle stimulating hormone profiles and their relationship with superovulatory response and quality of embryos in crossbred cows treated with Folltropin and prostaglandin F2. Indian Journal of Animal Sciences 71: 910–14.

Bonia K K and Goswami J. 2011.Serum 17ß- oestradiol, progesterone and cortisol level during biological stress period of normal and sub-oestrous cycles of crossbred cattle of Assam. Indian Journal of Animal Sciences 81: 676–78.

Chakravarty P, Bhuyan S, Sarmah B C and Baruah K K.2005. Serum oestradiol-17b and progesterone concentrations in superovulated local non-descript and crossbred cows of Assam. Indian Journal of Animal Physiology 1: 1–5.

Dhali A, Mishra D P, Mech A, Karunakaran M and Rajkhowa C. 2005. Endocrine control of estrous cycle in mithun (Bos frontalis). Theriogenology 64: 2010–21.

Dhali A, Mishra D P, Mech A, Karunakaran M and Rajkhowa C. 2006. Role of LH and prostaglandin F2a on the development and regression of corpus luteum in mithun (Bos frontalis) estrous cycle. General and Comparative Endocrinology 149: 173–81.

Kharche S D, Sharma G T, Agarwal S K, Majumdar A C, Sanwal P C and Sharma N C. 2001.Pre-treatment effect of human chorionic gonadotropin and estradiol-17β on ovarian response, embryo production and endocrine profile in cattle superovulated with FSH-P. Indian Journal of Animal Sciences 71: 644–49.

Kharche S D, Majumdar A C, Agarwal S K and Sharma G T.2008. Ovarian and endocrine response following pretreatment with human chorionic gonadotropin and estradiol valerate in PMSG treated crossbred cows. Indian Journal of Animal Sciences 78: 449–52.

Misra A K, Jaiswal R S, Kasiraj R, Mutha Rao M, Rangareddy N S, Pant H C.2000. Periovulatory steroid hormone profiles during unstimulated and superovulatory oestrous cycle in buffalo (Bubalus bubalis). Buffalo Journal 3: 371–80.

Mondal S and Prakash B S. 2003. Peripheral plasma progesterone concentration in relation to estrus expression in Sahiwal cows. Indian Journal of Physiology and Endocrinology 47: 111–14.

Nath K C, Dutta D J and Biswas R K. 2003.Progesterone and oestradiol profile in postpartum anoestrus cows following crestar and PMSG administration. Indian Journal of Animal Sciences 73: 1102–4.

Nilchuen P, Rattanatabtimtong S and Chomchai,S.2011. Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle. Songklanakarin Journal of Science and Technology 33: 679–83.

Novotny F, Hajurka J and Macak V. 2005. Relationship between blood serum progesterone levels in cattle donors and the yield and quality of embryos. Bulletin of the Veterinary Institute in Pulawy 49: 49–52.

Prasad S, Maurya S N and Varshney V P. 2003. Serum progesterone and estradiol –17ß concentration in relation to superovulatory response in embryo donor (Sahiwal) and conception rate in embryo recipient (crossbred) cattle. Indian Journal of Animal Sciences 73: 353–56.

Rao M M, Umamahesh Y and Misra A K.2010. Evaluation of ovarian response and embryo production pattern in Ongole cows. Indian Journal of Animal Sciences 80 (10): 973–75.

Singh S K, Agarwal S K and Shankar U. 1998. Effect of superovulatory response on embryo recovery and their quality in crossbred cattle. Indian Journal of Animal Reproduction 19: 1–3.

Sirohi N S. 2005. Plasma oestradiol-17β and progesterone profile in superovulated Hariana and Sahiwal cows. Indian Journal of Dairy Sciences 58: 105–88.

Snedecor G W and Cochran W G. 1989. Statistical Methods. 8th edn. Iowa State University Press, Ames, Iowa, USA.