Bovine Angptl6 gene effects on growth traits in three Chinese native cattle breeds

AIMIN LI; XIANYONG LAN; YUN MA; DONG LIU; HANFANG CAI; CHUZHAO LE; HONG CHEN

doi:10.56093/ijans.v82i11.25154

Authors

AIMIN LI College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
XIANYONG LAN College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
YUN MA College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
DONG LIU College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
HANFANG CAI College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
CHUZHAO LE College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
HONG CHEN College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China

https://doi.org/10.56093/ijans.v82i11.25154

Keywords:

Angptl6 gene, Cattle, Growth traits, Polymorphism

Abstract

As a circulating orphan peptide secreted by liver, Angptl6 gene might have great effects on growth and development of animals. The objective of this study was to explore sequence variants (SVs) of Angptl6 and to analyze their associations with growth traits in 737 individuals belonging to 3 Chinese native cattle. Herein, 3 variants were revealed by DNA pools sequencing and PCR-RFLP (or ACRS-PCR-RFLP) methods. These SVs were located at nt 2359, nt 2403 and nt 3258 (viz. SV1, SV2, SV3) respectively. All the analyzed populations were at Hardy-Weinberg equilibrium by c2-test. The linkage disequilibrium (LD) and haplotype analyses of 3 SVs revealed 8 different haplotypes. Thereinto, 2 dominant haplotypes (TCG and CCG) occurred with a frequency of 63.17% and 18.67% respectively. Association analysis showed the SV1 was significantly associated with BLI-6, and the SV2 and SV3 were significantly associated with CGI-24 and BHI-6 of individuals of Nanyang breed respectively. Further, combined SVs analysis revealed the ADG-18 of individuals with diplotype TC-CC-GG was higher than other combined genotypes in SV1-SV2-SV3 locus. Moreover, CG-18 with TC-GG and ADG-18 with TT-GT were preponderant in SV1-SV3 locus. Thus, 3 SVs showed great comprehensive effects on growth traits of Nanyang cattle, which indicated that they could be contributed to genetic and breeding programmes in cattle. Although there was no sufficient evidence these SVs greatly effect on the growth traits of cattle, the significant effects of these variants with these parameters could not be ignored.

Downloads

Download data is not yet available.

Author Biographies

AIMIN LI, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
XIANYONG LAN, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
YUN MA, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
DONG LIU, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
HANFANG CAI, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
CHUZHAO LE, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China
HONG CHEN, College of Animal Science and Technology, Northwest A&F University, Shaanxi; Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712 100 China

References

Ebert T, Bachmann A, Lossner U, Kratzsch J, Bluher M, Stumvoll M and Fasshauer M. 2009. Serum levels of angiopoietin-related growth factor in diabetes mellitus and chronic hemodialysis. Metabolism 58: 547–51.

Gale N W and Yancopoulos G D. 1999. Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes Development 13: 1055–66.

Gilbert R P, Bailey D R and Shannon N H. 1993. Linear body measurements of cattle before and after twenty years of selection for postweaning gain when fed two different diets. Journal of Animal Science 71: 1712–20.

Hato T, Tabata M and Oike Y. 2008. The role of angiopoietin-like proteins in angiogenesis and metabolism. Trends in Cardiovascular Medicine 18: 6–14.

Kadomatsu T, Tabata M and Oike Y. 2011. Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases. Febs Journal 278: 559–64.

Legry V, Goumidi L, Huyvaert M, Cottel D, Ferrières J, Arveiler D, Bingham A,Wagner A, Ruidavets J B and Ducimetiere P. 2009. Association between angiopoietin-like 6 (ANGPTL6) gene polymorphisms and metabolic syndrome-related phenotypes in the French MONICA Study. Diabetes & Metabolism 35: 287–92.

Nei M and Li W H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleaes. Proceedings of the National Academy of Sciences 76: 5269–73.

Norton N, Williams N M, Donovan M C and Owen M J. 2004. DNA pooling as a tool for large-scale association studies in complex traits. Annals of Medicine 36: 146–52.

Oike Y, Akao M, Yasunaga K, Yamauchi T, Morisada T, Ito Y, Urano T, Kimura Y, Kubota Y, Maekawa H, Miyamoto T, Miyata K, Matsumoto S, Sakai J, Nakagata N, Takeya M, Koseki H, Ogawa Y, Kadowaki T and Suda T. 2005. Angiopoietin-related growth factor antagonizes obesity and insulin resistance. Nature Medicine 11: 400–8.

Oike Y, Ito Y, Maekawa H, Morisada T, Kubota Y, Akao M, Urano T, Yasunaga K and Suda T. 2004. Angiopoietin-related growth factor (AGF) promotes angiogenesis. Blood 103: 3760–5.

Oike Y, Yasunaga K, Ito Y, Matsumoto S, Maekawa H, Morisada T, Arai F, Nakagata N, Takeya M, Masuho Y and Suda T. 2003. Angiopoietin-related growth factor (AGF) promotes epidermal proliferation, remodeling, and regeneration. Proceedings of the National Academy of Sciences 100: 9494–9.

Oike Y, Yasunaga K and Suda T. 2004. Angiopoietin-related/ angiopoietin-like proteins regulate angiogenesis. International Journal of Hematology 80: 21–8.

Orozco G, Hinks A, Eyre S, Ke X, Gibbons L J, Bowes J, Flynn E, Martin P, Wilson A G, Bax D E, Morgan A W, Emery P, Steer S, Hocking L, Reid D M, Wordsworth P, Harrison P, Thomson W, Barton A and Worthington J. 2009. Combined effects of three independent SNPs greatly increase the risk estimate for RA at 6q23. Human Molecular Genetics 18: 2693–9.

Sambrook J and Russell D W. 2002. Translated by huang pei tang. Molecular Cloning a Laboratory Manual. 3rd edn. Science Press. Beijing.

Zandbergen AA M, Lamberts S W J, Janssen J A M J L and Bootsma A H. 2006. Short-term administration of an angiotensin-receptor antagonist in patients with impaired fasting glucose improves insulin sensitivity and increases free IGF-I. European Journal of Endocrinology 155: 293–6.