Preliminary evaluation of papain: its enzymatic characteristics and effects on growth performance and nutrient digestibility in weaned piglets

BAOMING LING; DINGYUAN FENG; JIANJUN ZUO; ZHONGYUE ZHANG

doi:10.56093/ijans.v82i12.25696

Authors

BAOMING LING South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
DINGYUAN FENG South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
JIANJUN ZUO South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
ZHONGYUE ZHANG South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China

https://doi.org/10.56093/ijans.v82i12.25696

Keywords:

Enzymatic characteristics, Growth performance, Nutrient digestibility, Papain, Piglets, Weaned piglets

Abstract

The present study was conducted to investigate the influence of papain (Carica papaya L.) on performance and nutrient digestibility of weaned piglets and to evaluate whether soy protein ingredients supplemented with papain could replace part of high grade animal protein ingredients in weaned piglet feed. The results showed that Km, optimal pH and temperature of papain were 6.6875 mg/mL, 7.0° and 55°C respectively. It had a good stability when either pH ranged from 5.0 to 9.0 or temperature ranged from 4 to 65°C. The addition of papain at 75 mg/kg level significantly increased in vitro crude protein digestibility of soybean meal and piglet feed. Piglets (300; (Pietrain×Duroc)× (Landrace×Yorkshire)) weaned at 21±1 days with an initial body weight (BW) of 5.92±0.47 kg were assigned by BW, sex, and litter according to a randomized complete block design. The animal trial consisted of 5 dietary treatments with 6 replicates/treatment and 0 piglets/replicate. The dietary treatments were positive control (PC), negative control (NC), NC + 50 mg/kg papain, NC + 75 mg/kg papain, and NC +00 mg/kg papain. The results showed that papain significantly decreased diarrhea incidence of piglets fed the diets containing high level of soy-source protein material. There was a linearly improved apparent crude protein digestibility with the increasing dosage of protease. In conclusion, papain supplementation could be considered as an effective solution to break the inclusion limit of soybean source protein ingredients in weaned piglet diet.

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Author Biographies

BAOMING LING, South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
DINGYUAN FENG, South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
JIANJUN ZUO, South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China
ZHONGYUE ZHANG, South China Agricultural University, Guangzhou City, Guangdong Province, P.R. China

References

AOAC.1990. Official Methods of Analysis. 5th edn. Association of Official Analytical Chemists, Arlington, VA, USA.

Ashie I N A, Sorensen T L and Nielsen P M. 2002. Effect of papain and a microbial enzyme on meat proteins and beef tenderness. Journal of Food Science 67: 2138–42.

Bedford M R and Classen H L.1993. An in vitro assay for predication of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes. Poultry Science 72: 137–43.

Caine W R, Sauer W C, Tamminga S, Verstegen M W and Schulze H.1997. Apparent ileal digestibilities of amino acids in newly weaned pigs fed diets with protease-treated soybean meal. Journal of Animal Science 75: 2962–69.

Chen F Y and Ji P X. 2005. Studies on characteristics of immobilized papain on silk fibroin. Journal of South China Agricultural University 26 (4): 81–83 (in Chinese with English abstract).

Cone J W and van der Poel A F B.1993. Prediction of apparent ileal protein digestibility in pigs with a two-step in-vitro method. Journal of the Science of Food and Agriculture 62: 393–400.

Dierick N, Decuypere J, Molly K and Vanderbeke E. 2004. Microbial protease addition to a soybean meal diet for weaned piglets: effects on performance, digestion, gut flora and gut functions Recent advances of research in antinutritional factors in legume seeds and oilseeds/EAAP publication 4: 229–33.

Drew M D, Racz V J, Gauthier R and Thiessen D L. 2005. Effect of adding protease to coextruded flax: pea or canola: pea products on nutrient digestibility and growth performance of rainbow trout (Oncorhynchus mykiss). Animal Feed Science and Technology19: 117–28.

Li D F. 2003. Swine Nutrition. 2nd edn. China Agricultural Press, Beijing.

Ling B M, Feng D Y and Zuo J J. 2009. Progress in feed proteases. Feed industry 22: 7–10.

NRC.1998. Nutrient Requirements of Swine.10th edn. National Academic Press, Washington, DC.

O’Doherty J V and Forde S.1999. The effect of protease and -galactosidase supplementation on the nutritive value of peas for growing and finishing pigs. Irish Journal of Agricultural and Food Research 38: 217–26.

Pluske J R, Le Dividich J and Verstegen M W A. 2003. Weaning the Pig: Concepts and Consequences. Wageningen Academic Publishers, Netherlands.

Qu H Z, Huang L, Zhang G H, Chai Z, Zhang L, Hao D Y and Wang X P. 2008. The comparison of enzymatic characteristics of ficin and papain. Journal of Jilin University (Natural Science volume) 46 (6): 1217–20 (in Chinese with English abstract).

Rooke J A, Slessor M, Fraser H and Thomson J R.1998. Growth performance and gut function of piglets weaned at four weeks of age and fed protease-treated soya-bean meal. Animal Feed Science and Technology 70: 175–90.

SPSS.1995. SPSS Version 10.0 for Windows. SPSS, Inc. USA. Weetall H and Mason R.1973. Studies on immobilized papain. Biotechnology and Bioengineering 5 (3): 455–58.

Xiong Q C and Xu D C. 2008. Application progress of papain. China beet and sugar: 43–45 (in Chinese).

Yu B, Wu S T, Liu C C, Gauthier R and Chiou P W S. 2007. Effects of enzyme inclusion in a maize-soybean diet on broiler performance. Animal Feed Science and Technology 34: 283– 94.

Zyla K, Ledoux D R, Garcia A and Veum T.1995. An in vitro procedure for studying enzymic dephosphorylation of phytate in maize-soybean feeds for turkey poults. British Journal of Nutrition 74 (1): 3–17.