Effect of treatment of immunomodulators on recovery and conception rate in endometritic buffaloes

Abstract

A study was undertaken to evaluate the effect of administration of immunomodulators as an alternative to antibiotics
on the recovery of endometritis in buffaloes. Buffaloes (181) were diagnosed for endometritis were randomly grouped
to receive one of the following treatments; the animals of group 1 (n = 41) were treated with neem oil (Azadirachta
indica, 40 ml iu infusion twice at 24 h interval) and the buffaloes (40) in group 2 were administered with 100ug
Escherichia coli lipopolysaccharide intra uterine at similar times as in group 1. Blood plasma (40 ml) was infused via iu
route in animals (40) of group 3, whereas group 4 animals (40) received 20 ml of garlic extract at 2 occasions similar to
other groups. The control group 5 animals (20) were given only normal saline twice at 24 h interval. Efficacy of these
immunomodulators was assessed by negative reaction to whiteside test and a drastic reduction of bacterial load in
cervico-vaginal mucus (CVM) at subsequent estrus. The results indicated that administration of neem oil and E.coli
LPS retrieved cows from endometritis as majority of them showed negative colour reaction to whiteside test and a
significant decline of bacterial load in CVM. A satisfactory recovery rate (75%) in animals of group 3 and 4 was also
observed as compared to control (20%). Further, the maximum number of buffaloes were found pregnant (70.73%)
following neem oil-treatment. This study indicated therapeutic values of immunomodulators like neem oil, E.coli LPS,
blood plasma and garlic extract in endometritic buffaloes.