Isolation and characterization of neural stem cells from caprine

Abstract

Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transplantation is impractical
because it is hard to find a brain donor, and because of the low survival rate of grafted neural cells. Therefore, the need
for constructing a functional brain tissue is very critical. In this study neural stem cells were isolated and expanded from
caprine fetal brain in adherent culture. After 4–5 days of culture there were formation of small colonies and after about
10 days cells become confluent. Cells from passage 1 to 4 were used in study. These neural stem cells were characterized
with neural stem cell specific markers nestin (NES), PAX6 and SOX2 by PCR and immunocytochemistry. The cells were
expressed neural stem cell markers nestin, pax 6 and Sox 2.