Detection of oncogenic strains of MDV serotype 1 in Tamil Nadu by duplex PCR
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Authors
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KURUNCHI C DIVYA
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M PARTHIBAN
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G SATHISH
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K KUMANAN
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B MOHAN
Keywords:
Marek’s disease viruses, Meq gene, Polymerase Chain Reaction, 132 bp repeat sequenceAbstract
The present study was to increase the diagnostic sensitivity of PCR, duplex PCR for detection of Meq gene, and 132 bp repeat sequence of Marek’s disease virus (MDV) serotype 1 was used. Out of 60 samples collected from different suspected areas in Tamil Nadu, 8 samples showed the presence of amplified products of 1.06 kb (Meq gene) and 434bp (tandem repeats), whereas no amplification was observed in a virulent vaccine strain (serotype 3). Sequence homology of meq gene of these strains were confirmed with published sequences. These results clearly indicated that the detection of oncogenic strains of MDV serotype 1 in Tamil Nadu poultry populations.
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