Detection of virulence genes of Salmonella by polymerase chain reaction


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Authors

  • GEETIMA BARMAN
  • G K SAIKIA
  • D K BHATTACHARYYA
  • P ROYCHOUDHURY

Keywords:

PCR, Salmonella, Virulence genes

Abstract

Present investigation was conducted to screen the presence  of virulence genes stn, invA, sopB and sefC from 41
Salmonella isolates isolated from diarrheic as well as apparently healthy animals and birds. Thirty four (82.92%) belonged to Salmonella enterica serovar Typhimurium and    7 (17.07%) belonged to serovar S. Enteritidis. All the isolates detected positivity to stn gene, however invA, sopB and sefC were 63.41, 87.80 and 14.63% respectively. The sefC gene was present only in serovar Salmonella Enteritidis. More numbers of virulence genes could be detected among the isolates recovered from clinically affected animals/birds (34 isolates) in compare to the isolates from apparently healthy  (7) animal /birds.

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Author Biographies

  • GEETIMA BARMAN
    Research Associate, ICAR RC for NEH Region, Umroi Road, Umiam, Meghalaya.
  • G K SAIKIA
    Professor, Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India

  • D K BHATTACHARYYA
    Associate Professor, Department of Veterinary Microbiology, College of Veterinary Science.



  • P ROYCHOUDHURY
    Assistant Professor, Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, Mizoram

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Issue

Vol. 83 No. 5 (2013)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

BARMAN, G., SAIKIA, G. K., BHATTACHARYYA, D. K., & ROYCHOUDHURY, P. (2013). Detection of virulence genes of Salmonella by polymerase chain reaction. The Indian Journal of Animal Sciences, 83(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/29732