TIR domain of bovine TLR4 gene in Frieswal crossbred cattle: An early marker for mastitis resistance


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Authors

  • RAJIB DEB
  • UMESH SINGH
  • SUSHIL KUMAR
  • ARUN KUMAR
  • ARJAVA SHARMA
  • SANDEEP MANN
  • RANI SINGH

Keywords:

Frieswal cattle, Gene, Genotype frequency, TLR4, TIR

Abstract

Toll like receptor 4 (TLR4) is implicated as a receptor mediating cellular activation in response to bacterial lipopolysaccharides (LPS) of gram negative organisms which is the principal pathogen associated molecular patterns (PAMPs) of TLR 4. Thirty-one single nucleotide polymorphisms (SNP) were detected in bovine TLR4 gene. The SNP (g.9788C>T) found in the Toll IL-1 receptor (TIR) domain of the bovine TLR4 is reported to be associated with mastitis in earlier studies. In the present study we have amplified the TIR domain containing the above SNP site from Frieswal (Holstein Friesian × Sahiwal) crossbreed cattle blood genomic DNA. The amplified products of 126 unrelated Frieswal TIR domain of TLR4 gene were digested with Hinf I restriction enzyme and genotyping was analyzed. Our studies
revealed that CC alleles (0.97) are more prevalent than TT alleles (0.03) in Frieswal genome.

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Author Biographies

  • RAJIB DEB
    Scientist, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • UMESH SINGH
    Principal Scientist, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • SUSHIL KUMAR
    Senior Scientist, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • ARUN KUMAR
    Senior Scientist, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • ARJAVA SHARMA
    Director, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • SANDEEP MANN
    Senior Research Fellow, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

  • RANI SINGH
    Research Associate, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh 252 120 India

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Issue

Vol. 83 No. 6 (2013)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DEB, R., SINGH, U., KUMAR, S., KUMAR, A., SHARMA, A., MANN, S., & SINGH, R. (2013). TIR domain of bovine TLR4 gene in Frieswal crossbred cattle: An early marker for mastitis resistance. The Indian Journal of Animal Sciences, 83(6). https://epubs.icar.org.in/index.php/IJAnS/article/view/30648