Cytopathogenicity of buffalopox and camelpox virus in buffalo fibroblast cells

T ANAND; B C BERA; RIYESH T; R K VAID; S BARUA; NITIN VIRMANI; PRAVEEN MALIK; D KUMAR; P S YADAV; R K SINGH

doi:10.56093/ijans.v83i12.35792

Authors

T ANAND Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
B C BERA Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
RIYESH T Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
R K VAID Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
S BARUA Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
NITIN VIRMANI NRCE, Hisar, National Research Centre on Equines, Hisar, Haryana 125 001 India
PRAVEEN MALIK Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India
D KUMAR Central Institute for Research on Buffaloes, Hisar, National Research Centre on Equines, Hisar, Haryana 125 001 India
P S YADAV Central Institute for Research on Buffaloes, Hisar, National Research Centre on Equines, Hisar, Haryana 125 001 India
R K SINGH Veterinary Type Culture Centre, National Research Centre on Equines, Hisar, Haryana 125 001 India

https://doi.org/10.56093/ijans.v83i12.35792

Keywords:

Buffalopox, Camelpox, Fibroblast

Abstract

The primary cultures of fibroblast cells were established from the ear marginal tissue of a young calf of buffalo (Bubalus bubalis). Subsequent passages of primary fibroblast cells were carried out upon 80–90% confluency. The cells were found to be chromosomally stable and free of Mycoplasma contamination. Housekeeping genes namely- GAPDH and β-Actin were amplified from fibroblast cells. To check the utility of developed fibroblast cells in adaptation of virus, cell monolayers were infected with buffalopox virus (BPXV) and camelpox virus (CMLV) isolates, which showed typical virus-specific CPE. The infection was confirmed by PCR amplification of BPXV and CMLV-specific region of C18L gene. The cell line thus developed could be of immense potential in propagation of viruses adopting skin route of infection and their immuno-modulatory associations.

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