Flow cytometric sorting of Sahiwal bulls semen coupled with artificial insemination at organized herd
138 / 42
Keywords:
Artificial insemination, Cryopreservation, Flow cytometry, Sahiwal, Sperm sortingDownloads
References
Bloom E. 1950. A one-minute live-dead sperm stain by means of Eosine-Nigrosin. Fertility and Sterility 1:176–77. DOI: https://doi.org/10.1016/S0015-0282(16)30125-X
Carvalho J O, Sartori R, Machado G M, Mourão G B and Dode M A. 2010. Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production. Theriogenology 74(9):1521–30. DOI: https://doi.org/10.1016/j.theriogenology.2010.06.030
Duarte A M, Diniz E G, Nascimento R M B M, Tavares M and Franco R V R. 2007. Pregnancy rates in heifers inseminated with semen of Nelore bulls sexed by flow cytometry. Ciencia Animal Brasileira 8 (3): 567–73.
Garner D L and Seidel Jr G E. 2003. Past, present and future perspectives on sexing sperm. Canadian Journal of Animal Science 83: 375–84. DOI: https://doi.org/10.4141/A03-022
Garner D L. 2006. Flow cytometric sexing of mammalian sperm. Theriogenology 65: 943–57. DOI: https://doi.org/10.1016/j.theriogenology.2005.09.009
Garner D L and Seidel Jr. G E. 2008. History of commercializing sexed semen for cattle. Theriogenology 69: 886–95. DOI: https://doi.org/10.1016/j.theriogenology.2008.01.006
Jeyendran R S, Vander Ven H H, Perez-Pelaez M, Crabo B G and Zaneveld L J D. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70: 219–25. DOI: https://doi.org/10.1530/jrf.0.0700219
Johnson L A and Welch G R. 1999. Sex preselection: High-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology 52: 1323– 41. DOI: https://doi.org/10.1016/S0093-691X(99)00220-4
Johnson L A, Welch G R and Rens W. 1999. The Beltsville sperm sexing technology: High speed sperm sorting gives improved sperm output for in vitro fertilization and A.I. Journal of Animal Science 77(2): 213–20. DOI: https://doi.org/10.2527/1999.77suppl_2213x
Maxwell W M C, Evans G, Hollinshead F K, Bathgate R, de Graaf S P, Eriksson B M, Gillan L, Morton K M and O’ Brien J K. 2004. Integration of sperm sexing technology into the ART toolbox. Animal Reproduction Science 82–83: 79–95. DOI: https://doi.org/10.1016/j.anireprosci.2004.04.013
Nebel R, DeJarnette J M and De vries A. 2009. Effects of sexed semen on replacement numbers and long term economics. Proceedings of 13th Annual DCHA Dairy Calf and Heifer Conference. Pp. 23–36. Tucson, AZ.
Norman H D, Hutchison J L and Miller R H. 2010. Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holsteins in the United States. Journal of Dairy Science 93: 3880–90. DOI: https://doi.org/10.3168/jds.2009-2781
Schenk J L, Suh T K, Cran D G and Seidel Jr. G E. 1999. Cryopreservation of flow-sorted bovine spermatozoa. Theriogenology 52: 1375–91. DOI: https://doi.org/10.1016/S0093-691X(99)00224-1
Seidel Jr G E and Garner D L. 2002. Current status of sexing mammalian spermatozoa. Reproduction 124: 733–43. DOI: https://doi.org/10.1530/rep.0.1240733
Seidel Jr G E and Schenk J L. 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: Effects of sperm numbers per inseminates and site of sperm deposition. Animal Reproduction Science 105(1):129–38. DOI: https://doi.org/10.1016/j.anireprosci.2007.11.015
Watson P F. 1975. Use of Giemsa stain to detect change in the acrosome of frozen ram spermatozoa. Veterinary Record 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12
Welch G R and Johnson L A. 1999. Sex preselection: Laboratory validation of the sperm sex ratio of flow sorted X-and Y-sperm by sort reanalysis for DNA. Theriogenology 52: 1343–52. DOI: https://doi.org/10.1016/S0093-691X(99)00221-6
Wilson R D, Fricke P M, Leibfried-Rutledge M L, Rutledge J J, Penfield C M and Weigel K A. 2006. In vitro production of bovine embryos using sex-sorted sperm. Theriogenology 65(6):1007–15. DOI: https://doi.org/10.1016/j.theriogenology.2005.07.007
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.